Examinations on the changes in oviduct epithelium during the sexual cycle by light and electron microscope in Akkaraman sheep

WOS: 000309621500003The aim of the study was to examine the changes in the oviduct epithelium during the sexual cycle by light and electron microscope in Akkaraman sheep. In this study; 10 oestral (follicular phase), 10 luteal and 5 anoestral phase samples were collected from the infundibulum, ampulla and istmus regions of oviduct from a total of 25 healthy Akkaraman sheeps having reached their puberty. In the light microscopy, it was also observed that, in oestral phase; height of the cells were higher than luteal phase and in the luteal phase nucleus extrusion were seen in the ampulla and infundibulum. It was detected that findings of the anoestrus phase were similar to dioestrus phase. Amount of secrets of secretory cells in the ampulla were elevated during the oestral phase which was determined by histochemical stains. During the oestral phase by using electron microscope, three types of granules were seen in the secretory cells which were especially located in the ampulla. First type of granule was electron lucent and had moderately electron dense substance which was located separetely, right below the granule membran. Second type of granule had carried electron dense focus which had consantric lamels around of. Some of these types of granules were also seen located eccentric to electron dense focus. Third type of granules, which don't have electron dense focus at the centre, are with consantric lamels. This study showed the histological changes on the oviduct epithelium of Akkaraman sheeps during sexual cycle. The amount and the characterization of the secret in the secretory cells, varied upon the cycle period and the region of the oviduct

Ultrastructural characteristics of sheep and horse mesenchymal stem cells (MSCs)

In the present study, the morphological and ultrastructural characteristics of mesenchymal stem cells (MSCs) induced towards osteogenic, adipogenic, and chondrogenic lineages were investigated. The main objective of this paper was to focus on the differentiation capacity of ovine [oMSCs] and equine MSCs [eMSCs]. Bone marrow [BM] MSCs were isolated from ovine and equine patients, expanded in monolayer culture and induced into osteogenic, adipogenic and chondrogenic differentiation. For chondrogenic differentiation, cells were cultured in micromass culture. Also, their ultrastructural phenotypes were studied by transmission electron microscopy [TEM]. This study was aimed to provide an indepth morphological description of BM-derived ovine and equine MSCs induced towards three lineages, it demonstrated that TEM analysis is useful in elucidating detailed structural information

Light and electron microscopic studies on the oviduct epithelium of the Pekin duck (Anas platyrhynchos)

WOS: 000267672400004The present study was undertaken to investigate the histochemical properties and light and electron microscopic morphology of the oviduct of laying Pekin ducks and Pekin ducks in the quiescent phase of the reproductive Cycle. The oviducts of five healthy, two-year-old adult laying Pekin ducks and five five-month-old Pekin ducks in the quiescent phase of the reproductive cycle, obtained from the Farm of Ankara University Faculty of Veterinary Medicine, constituted the Material of the present study. The oviduct of the Pekin duck was segmented into five regions. namely, the infundibulum, magnum, isthmus, uterus and vagina. Along the oviduct, the tunica mucosa forms convolutions protruding into the lumen, and the height of these convolutions were determined to increase in the regions of the magnum and uterus. The lamina epithelialis was ascertained to be composed of ciliated and secretory cells. The lamina propria was filled with glands in the magnum, isthmus and uterus. In laying ducks, the PAS-positive reaction in the isthmus and the performic acid/Alcian blue pH 2.5 reaction in the magnum were determined to tic very strong. Oil the other hand, due 10 the cells forming the lamina epithelialis and the proprial glands not having developed fully, these reactions were observed to be weak in ducks in the quiescent phase of the reproductive cycle. In electron microscopic examinations, a single type of secretion granule, of medium electron density and varying size, was observed in the secretory cells of the lamina epithelialis. Electron dense secretion granules were present in the proprial glands of the magnum and isthmus, whereas proprial glands of the Uterus contained electron light granules

Histomorphology of the oviduct epithelium in the Angora rabbit

WOS: 000279893900001The present study was undertaken to investigate cyclic changes in the structure of oviduct epithelial cells, and the content of oviductal mucus in the Angora rabbit using light and electron microscopy. Ten female Angora rabbits, 5 of which were in the estrual stage and the other 5 were in the luteal stage of the estrous cycle, were used in the study. Tissue samples taken from the fimbria, ampulla and isthmus of the oviduct were examined under light and electron microscope. Ciliated cells were demonstrated to be predominant in the fimbria and ampulla, whereas secretory cells were determined to be most numerous in the isthmus. The estrual stage was characterized with greater cell heights, and increased numbers of ciliated cells and secretion. Both secretion and cilia were determined to decrease evidently in the luteal stage. Neutral and acidic mucosubstances were found to be present in the ampulla and isthmus. In the isthmus, in which secretion was dense, acidic mucosubstance was determined to contain sulphate and carboxyl groups by means of combined Aldehyde fuchsin/Alcian blue (AF/Ab) staining. Electron microscopic examination revealed the presence of electron-dense and electron-light secretion granules in secretory cells. In all cyclic stages, electron-dense foci were observed in the electron-light secretion granules of some secretory cells

Investigation of Mast Cell Distribution in the Ovine Oviduct During Oestral and Luteal Phases of the Oestrous Cycles

WOS: 000344690200015Mast cells are heterogeneous cell populations that play significant roles in many organs and systems and involve various physiological processes. We aimed to evaluate mast cells in the ovine oviduct mucosa by means of their staining and ultrastructural characteristics. The ovine oviduct samples of Akkaraman breed were collected from the slaughterhouse and they are categorized as luteal and oestral phases. They were fixed either with 10% formalin or IFAA and stained with Toluidine blue and Alcian blue and Safranin O (Ab/SO). Mast cells were located near blood vessels and basal membrane. Compared to 10% formalin fixed tissues, the number of mast cells were higher in IFAA fixed tissues (P=0.003). Importantly all mast cells Ab(+) and SO(-) so that they were categorized as mucosal type. The number of mast cells did not differ between luteal and oestral phases (P>0.05). However, there were significant differences among different regions of the oviduct with a less count in the isthmus regions (P=0.006). Transmission electron microscopy revealed that the oviduct mast cells contained two types of granules: an electron lucent, electron dense. Some electron lucent granules contained an eccentrically located crystal-like structure. The significance of less mast cell counts in the isthmus and the eccentrically located single crystal-like structure should be further investigated in future studies

Highly personalized information delivery to mobile clients

The inherent limitations of mobile devices necessitate information to be delivered to mobile clients to be highly personalized according to their profiles. This information may be coming from a variety of resources like Web servers, company intranets, email servers. A critical issue for such systems is scalability, that is, the performance of the system should be in acceptable limits when the number of users increases dramatically. Another important issue is being able to express highly personalized information in the user profiles, which requires a querying power as that of SQL on relational databases. Finally, the results should be customized according to user needs, preferences and the mark up language of their mobile device. Since the queries will be executed on the documents fetched over the Internet, it is atural to expect the documents to be XML documents

Studies on the morphology, histochemistry and ultrastructure of Paneth cells in the small intestine of the Angora rabbit

WOS: 000261921500005The present study was undertaken to determine the morphology, histochemical properties, localization and quantitative distribution of Paneth cells in the small intestine of the Angora rabbit. Tissue samples taken from the duodenum. jejunum and ileum of 10 healthy Angora rabbits, obtained from private breeders, constituted the material of the study. The Paneth cells, which were determined to be located within the crypts of Lieberkuhn, were identified on their basally located nucleus and apically located acidophilic granules. These granules gave positive reactions with Mallory's triple staining, technique as well as with the application of Phloxine-tartrazine, Alcian blue-performic acid and Mallory's phosphotungstic acid-hematoxylin method. Staining with periodic acid-Schiff (PAS). Alcian blue (pH 2.5), and PAS-Alcian blue gave negative reactions. Paneth cells were determined not to display a uniform distribution throughout the small intestine and cell numbers were ascertained to show a gradual increase from the duodenum towards the ileum. The difference between the three regions of the small intestine was determined to he statistically significant (p<0.01). Electron microscopic examination revealed the presence of electron-dense and homogenous granules in the apical cytoplasm of some Paneth cells, whereas homogenous granules of different electron density existed in some other cells

Morphological studies on ovarian mast cells in the cow

WOS: 000247802900008This study was conducted on cows in the estrual and luteal phases of the cycle to determine the staining properties, light and electron microscopic appearance and numerical distribution of mast cells from various areas of the ovary. The material for the study comprised ovarian specimen taken from 14 cows, 7 in the luteal phase and 7 in the estrual phase of the sexual cycle. Specimens were taken from 3 different areas of the ovaries, including those surrounding the corpus luteum, the graafian follicle and the medulla. From all 3 areas, mast cells were demonstrated as metachromatic staining after treatment of paraffin- embedded sections with toluidine blue and as Ab(+)/ SO(-) by the combined alcian blue/ safranine O (Ab/ SO) stain. In the electron microscopic studies, the mast cells were observed to have 2 types of granules, namely homogeneously dense granules and tiny particulate granules. All 3 regions of the ovary were observed to have higher average mast cell counts per mm 2 during the estrual phase than during the luteal phase (P < 0.001). In the estrual phase, while mast cell counts demonstrated a marked increase in the periphery of the graafian follicle, a low number of mast cells was determined in the periphery of the corpus luteum. In comparison, the medulla was demonstrated to have a much higher concentration of mast cells in the medulla than the other 2 regions of the ovarium

Ultrastructure Of Rat Umbilical Cord Stroma-Derived Mesenchymal Stem Cells

Being multipotent progenitor cells, mesenchymal stem cells continue to attract attention as a promising tool for cell-based therapy and tissue engineering. However, research on the ultrastructure of these cells is rather scarce. This study was aimed at the investigation of the transmission electron microscopic ultrastructure and morphology of mesenchymal stem cells isolated from the stroma of rat umbilical cord using the explant culture method. At the end of the third passage, some of the cells isolated from the stroma of the umbilical cord were harvested for electron microscopic examination. The remaining cells were induced for osteogenic, chondrogenic, and adipogenic differentiation. On the basis of the results obtained, it was concluded that mesenchymal stem cells derived from the stroma of the umbilical cord display morphological features similar to those of other types of mesenchymal stem cells and can be used as an alternative cell source in cell-based therapy owing to the advantages they offer.WoSScopu