Isolation of Biosynthetically Blocked Mutants from Anthracycline. Producing Streptomyces Violaceus A262 and Streptomyces sp. D788 and an Attempt on the Interspecific Recombination between Them by Protoplast fusion

ロドマイシン群抗生物質生産菌 Streptomyces violaceus 及びダウノマイシン生産菌Streptomyces sp. D788 の変異処理により、新規生合成中間体を蓄積する生合成ブロック変異株12種を単離した。生成物の薄層クロマグラフィーの結果から、7株は生合成初期段階がブロックされ前駆体アグリコン類を蓄積する変異株、5株はグリコシル化以降の段階でブロックされグリコシド中間体を蓄積する変異株であった。また、両菌種間で生合成ブロック変異の組換えをおこなう目的でプロトプラスト融合による異種間遺伝子組換えを試みたが起こらなかった。Tweleve mutants which produced novel biosynthetic anthracycline metabolites were obtained from two anthracycline producers, rhodomycin-producing Streptomyces violaceus A262 and daunomycin-producing Streptomyces sp. D788. by mutagenic treatment. The seven mutants were precursor aglycone-accumulating ones and the five mutants were glycosidic intermediate-accumulating ones. Five anthracycline-nonproducing mutants were also isolated. Interspecific recombination between these two Streptomyces strains is other important means for obtaining a diserbale biosynthetic mutant if it is possible. However, it was found that no recombination occurred between them