207 research outputs found
Evaluation of focal cartilage lesions of the knee using MRI T2 mapping and delayed Gadolinium Enhanced MRI of Cartilage (dGEMRIC)
Leukotrienes inhibit early stages of HIV-1 infection in monocyte-derived microglia-like cells
<p>Abstract</p> <p>Background</p> <p>Microglia are one of the main cell types to be productively infected by HIV-1 in the central nervous system (CNS). Leukotriene B<sub>4 </sub>(LTB<sub>4</sub>) and cysteinyl-leukotrienes such as LTC<sub>4 </sub>are some of the proinflammatory molecules produced in infected individuals that contribute to neuroinflammation. We therefore sought to investigate the role of leukotrienes (LTs) in HIV-1 infection of microglial cells.</p> <p>Methods</p> <p>To evaluate the role of LTs on HIV-1 infection in the CNS, monocyte-derived microglial-like cells (MDMis) were utilized in this study. Leukotriene-treated MDMis were infected with either fully replicative brain-derived HIV-1 isolates (YU2) or R5-tropic luciferase-encoding particles in order to assess viral production and expression. The efficacy of various steps of the replication cycle was evaluated by means of p24 quantification by ELISA, luciferase activity determination and quantitative real-time polymerase chain reaction (RT-PCR).</p> <p>Results</p> <p>We report in this study that virus replication is reduced upon treatment of MDMis with LTB<sub>4 </sub>and LTC<sub>4</sub>. Additional experiments indicate that these proinflammatory molecules alter the pH-independent entry and early post-fusion events of the viral life cycle. Indeed, LT treatment induced a diminution in integrated proviral DNA while reverse-transcribed viral products remained unaffected. Furthermore, decreased C-C chemokine receptor type 5 (CCR5) surface expression was observed in LT-treated MDMis. Finally, the effect of LTs on HIV-1 infection in MDMis appears to be mediated partly via a signal transduction pathway involving protein kinase C.</p> <p>Conclusions</p> <p>These data show for the first time that LTs influence microglial cell infection by HIV-1, and may be a factor in the control of viral load in the CNS.</p
Peptidergic Innervation in the Cerebral Blood Vessels of the Guinea Pig: An Immunohistochemical Study
Origins and Pathways of Cerebrovascular Vasoactive Intestinal Polypeptide-Positive Nerves in Rat
Isobutyl 2-cyanoacrylate (bucrylate) in obliteration of gastric coronary vein and esophageal varices
Powerformer™: A giant Step in Power Plant Engineering
Powerformer(R), a new high-voltage generator, was presented by ABB. It offers a direct connection to the power network without the need for a step-up transformer. In a sense, Powerformer incorporates into one unit the functions of both the conventional generator and the step-up transformer. Thanks to its ability to generate electricity at transmission voltage levels, a power plant utilizing Powerformer offers considerable gains with respect to reactive power production and plant efficiency. Thus, Powerformer both facilitates network stability and decreases the exploitation of the natural resource
The effect of 17-B-estradiol on the fine structure of the adrenergic axons of the rabbit myometrium
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