8 research outputs found
Photoaccelerated Complex Formation of a Novel Langmuir−Blodgett Film with Transition Metal Ions and Its Ion Selectivity
Adsorption Behavior and Structural Characterization of Azo Dyes on a Langmuir−Blodgett Film of Octadecylamine
Contribution of Lateral Force and “Tapping Mode” Microscopies to the Study of Mixed Protein Langmuir−Blodgett Films
Immobilization of Bioactive Protein A from Staphylococcus aureus (SpA) on the Surface of Bacillus subtilis Spores
Oriented immobilization of proteins
Immobilized enzymes have found numerous applications in analytical, clinical, environmental and industrial chemistry. However, in most cases, immobilization leads to partial or total loss of activity. It is widely believed that the loss in activity is due to attachment of proteins on the immobilization support through several amino acid residues. This results in a random orientation of the immobilized protein and in increased structural deformation due to multi-point attachment. Several researchers have explored ways to orient proteins on surfaces, such that orderly organization, single point attachment and accessibility of the active site (or binding site) are possible. This article reviews the various approaches available to achieve oriented immobilization of proteins and its applications in several disciplines