1 research outputs found
PPARĪ± contributes to protection against metabolic and inflammatory derangements associated with acute kidney injury in experimental sepsis
Abstract Sepsisāassociated acute kidney injury (AKI) is a significant problem in critically ill children and adults resulting in increased morbidity and mortality. Fundamental mechanisms contributing to sepsisāassociated AKI are poorly understood. Previous research has demonstrated that peroxisome proliferatorāactivated receptor Ī± (PPARĪ±) expression is associated with reduced organ system failure in sepsis. Using an experimental model of polymicrobial sepsis, we demonstrate that mice deficient in PPARĪ± have worse kidney function, which is likely related to reduced fatty acid oxidation and increased inflammation. Ultrastructural evaluation with electron microscopy reveals that the proximal convoluted tubule is specifically injured in septic PPARĪ± deficient mice. In this experimental group, serum metabolomic analysis reveals unanticipated metabolic derangements in tryptophanākynurenineāNAD+ and pantothenate pathways. We also show that a subgroup of children with sepsis whose genomeāwide expression profiles are characterized by repression of the PPARĪ± signaling pathway has increased incidence of severe AKI. These findings point toward interesting associations between sepsisāassociated AKI and PPARĪ±ādriven fatty acid metabolism that merit further investigation