Study regarding coffee brew metal content

This paper contains data regarding the concentration of some essential mineral elements in certain coffee brews and their mineral supply in the daily-recommended diet. We determined, through flame atomic absorption spectrometry, the concentration of Na, K, Ca, Mg, Fe, Mn, Zn and Cu in brews made with five assortments of coffee. The mean values of the concentrations determined experimentally – 34.90 mg/L (Ca), 21.49 mg/L (Mg), 1.180 mg/L (Na), 522 mg/L (K), 0.158 mg/L (Fe), 0.206 mg/L (Mn), 0.146 mg/L (Zn), 0.027 mg/L (Cu) – allowed the calculus of their mineral supply. Data show that an intake of 300 mL coffee brew covers in a small measure the daily recommended intake of essential elements; therefore, coffee brews cannot be taken into account as far as their mineral supply is concerned

DNA Polymorphisms and mRNA Levels of Immune Biomarkers as Candidates for Inflammatory Postpartum Disorders Susceptibility in Italian Buffaloes

The immunological genes that may interact with inflammatory postpartum diseases in Italian buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal and 60 with inflammatory reproductive diseases) were employed. Each buffalo’s jugular vein was pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological (IKBKG, LGALS, IL1B, CCL2, RANTES, MASP2, HMGB1, and S-LZ) genes’ nucleotide sequence differences between healthy buffaloes and buffaloes affected by inflammatory reproductive diseases were found by employing PCR-DNA sequencing. According to Fisher’s exact test (p ˂ 0.01), there were noticeably different probabilities of all major nucleotide changes spreading among buffalo groups with and without reproductive problems. Buffaloes were significantly more likely to express the examined genes when they had inflammatory reproductive diseases. The outcomes might support the significance of these markers’ nucleotide variations and gene expression patterns as indicators of the prevalence of inflammatory reproductive disorders and provide a workable buffalo management policy