20 research outputs found
Patterns of natural selection acting on the mitochondrial genome of a locally adapted fish species
ContribuiçÔes dos registros eletrÎnicos para a segurança do paciente em terapia intensiva: uma revisão integrativa
Quantification of topoisomerase-DNA complexes in leukemia cells from patients undergoing therapy with a topoisomerase-directed agent
Multifactor dimensionality reduction analysis identifies specific nucleotide patterns promoting genetic polymorphisms
Template strand scrunching during DNA gap repair synthesis by human polymerase λ
Family X polymerases such as DNA polymerase (Pol ) are well suited for filling short gaps during DNA repair because they simultaneously bind both the 5' and 3' ends of short gaps. DNA binding and gap filling are well characterized for 1-nucleotide (nt) gaps, but the location of yet-to-be-copied template nucleotides in longer gaps is unknown. Here we present crystal structures revealing that, when bound to a 2-nt gap, Pol scrunches the template strand and binds the additional uncopied template base in an extrahelical position within a binding pocket that comprises three conserved amino acids. Replacing these amino acids with alanine results in less processive gap filling and less efficient NHEJ when 2-nt gaps are involved. Thus, akin to scrunching by RNA polymerase during transcription initiation, scrunching occurs during gap filling DNA synthesis associated with DNA repair