Evaluation of Zerofly® Hermetic Storage Bags for Protection of Maize Against Insect Pests in Ghana

High post-harvest loss of maize due to stored product insect pests remains a food security challenge in Ghana. This field study evaluated the effectiveness of a novel technology, ZeroFly® Hermetic storage bags with different inner liners, to protect maize against infestation by Sitophilus zeamais Motschulsky and Prostephanus truncatus Horn. The study was carried out in the Department of Crop and Soil Sciences, Kwame Nkrumah University of Science and Technology, Ghana, during the period September 2017 to March 2018. Maize pre-fumigated with Phostoxin® was stored in 50-kg ZeroFly® Hermetic storage bags. Experimental treatments were 20-μm High Density Polyethylene (HDPE) inner liner with oxygen absorber, 20-μm HDPE inner liner without oxygen absorber, 80-μm Polyester and Ethylene Vinyl Alcohol (EVOH) inner liner with oxygen absorber, 80-μm EVOH inner liner without oxygen absorber, 50-μm Charter NEX high barrier inner liner with oxygen absorber, 50-μm Charter NEX medium barrier inner liner with oxygen absorber, and untreated maize-filled 50-kg polypropylene bags without oxygen absorber (control). Maize-filled 50-kg polypropylene bags without oxygen absorber were used as Control. The percent insect damaged kernels on number basis (%IDKnb) recorded in the 20-μm HDPE liners and polypropylene bags were \u3e 15% compared to \u3c 1.8% recorded in the other treatments. Weight losses in the 80-μm EVOH and 50 -μm Charter NEX high and medium barrier liners were \u3c 0.35%. Aflatoxin levels were below the 15 ppb threshold. The results of the study showed that, ZeroFly® Hermetic storage bags with 80-μm EVOH inner liner and 50-μm Charter NEX high and medium barrier inner liners protected stored maize against S. zeamais and P. truncatus over the 6-month storage period

Collection of edible wild fruits in Eastern Region of Ghana

Edible wild fruits were collected in the Eastern Region of Ghana. Interviews and group discussions were the methods used to know the locations and subsequent collecting of the fruits. Twelve accessions of fruits were collected of which most were from trees. Cash crop (cocoa) farms were the habitats for most fruit trees in the region. Chrysophyllum albidum and some trees were deliberately planted in farms for income and sale. Some fruit trees were being domesticated.La collection des fruits sauvages comestibles était entreprise dans la région de l'Est du Ghana. Les entrevues et les discussions en groupe étaient les méthodes adoptées pour connaître les emplacements et par la suite, la collecte des fruits. Douze accessions des fruits étaient ramassées dont la plupart étaient cueillies des arbres. Les champs de culture commerciale (cacao) étaient les habitats pour la plus grandes parties des arbres fruitiers dans la région. Chrysophyllum albidum et quelques arbres étaient délibérément plantés aux champs pour le revenu et le commerce. Certains des arbres fruitiers étaient en train d'être domestiqués. Ghana Journal of Agricultural Science Vol. 40 (2) 2008: pp. 165-16

Insecticide Use Practices in Cocoa Production in Four Regions in Ghana

Chemical control of insect pests of cocoa started in 1950, and insecticides from the various classes have been recommended and used by farmers since then. Presently, Imidacloprid (Confidor®), Bifenthrin (Akatemaster®) and Thiamethoxam (Actara®) are recommended by Ghana Cocoa Board (COCOBOD) for insect pest management. A survey was conducted in the Ashanti, Eastern, Volta and Western regions of Ghana using questionnaires and farm visits of 147 cocoa farmers’ fields to gather information on insecticide use practices by farmers. The survey showed that the farmers used mostly Imidacloprid and Bifenthrin insecticides and the frequency of application was more than that recommended by COCOBOD. Among the three recommended insecticides, 43% each of the farmers across the three regions used either Confidor® or Akatemaster® whilst the remaining 14% used Actara®. The number of years farmers had consistently used a particular insecticide ranged between 5 and 16 years. Whilst some cocoa farmers do not apply insecticides to their farms, others, however, do as many as 11 applications in a year. Most of the insecticides used are classified as class II under WHO Hazard category, and the farmers used very minimal protective clothing during pesticides application. The results of this study show that there is the need to intensify education on safe handling and use of pesticides to reduce pesticide abuse, especially by cocoa farmers, in order to sustain effective management of pests and protect farmers, consumers and the environment

Management of Insect Pests using Chlorpyrifos Applications at Different Growth Stages of Tomato, Solanum lycopersicum L.

Field studies were conducted in Kumasi, Ghana in the major and minor planting seasons of 2013 to evaluate the effect of chlorpyrifos applications at different growth stages of tomato (Solanum lycopersicum L) for the management of insect pests. The treatments were: Chlorpyrifos at 1.5 ml / 0.5 litre of water applied (i) at the vegetative growth stage only (sprayed vegetative), (ii) at the reproductive growth stage only (starting at 50 % flowering (sprayed reproductive) and (iii) throughout the crop’s growth period (sprayed throughout). A control plot that received only water was also maintained. In both seasons, significantly (P < 0.05) less number of Bemisia tabaci Gennadius and Thrips tabaci Linderman were collected from the insecticide-treated tomato plots than the untreated, control plots, but no significant differences were observed among the treatments in the aggregations of Aphis gossypii Glover and Helicoverpa armigera (Hubner). Chlorpyrifos application in the sprayed throughout plots significantly (P < 0.05) reduced damage to tomato fruits in the major season but not in the minor season. Tomato fruits from the sprayed vegetative and sprayed reproductive plots had comparable damage as the fruits from untreated control plots. Chlorpyrifos application also significantly (P < 0.05) increased yield of tomato from the sprayed throughout plots in both seasons. The best protection to the crop against the insect pests was obtained from weekly applications of chlorpyrifos throughout the growth period of the crop (sprayed throughout plots). Key words: Tomato, Chlorpyrifos, insect pests, management, population dynamic

Evaluation of Insecticides for the Management of Insect Pests of Tomato, Solanum Lycopersicon L.

Field studies were undertaken in Kumasi, Ghana in 2012 and 2013 to evaluate the efficacy of two insecticides i.e. Lambda Super 2.5 EC (a.i- lambda cyhalothrion) and Cymethoate Super EC (a. i.-cypermethrin and cymethoate) for the management of insect pests on tomato, Solanum lycopersicum L. Lambda Super was applied at 1.5 ml/0.5 L of water whilst Cymethoate Super was applied at 0.25 ml/0.5 L of water. A control plot (water application only) was also maintained. Whiteflies, Bemisia tabaci (Gennadius), thrips, Thrips tabaci Lindeman, aphids, Aphis gossypii (Glover), leaf miners, Liriomyza sp. and the tomato fruit worm, Helicoverpa armigera (Hubner) were the most important insect pests collected on tomato in the area. In 2012, there were no significant differences among the insecticide-treated plots and the control with respect to the densities of B. tabaci, A. gossypii, Liriomyza sp. and H. armigera. Cymethoate Super treated plots recorded significantly lower number of T. tabaci than the control plots. However, in 2013, the control plots recorded significantly more aggregations of B. tabaci, H. armigera and A. gossypii than the Lambda Super and Cymethoate Super treated plots. No significant differences were obtained in the numbers of Liriomyza sp. and T. tabaci among the treatments. In 2012, the insecticide-treated plots recorded significantly higher fruit yield than the control but there were no significant differences among the treatments with respect to percent damaged fruits and mean shoot dry weight in 2013. Lambda Super and Cymethoate Super can be used to manage insect pests on tomato for increased yield. Keywords: Insecticides, efficacy, tomato pests, population densities, yiel

INSECTICIDE HANDLING IN COCOA PRODUCTION IN FOUR REGIONS IN GHANA

Management of insect pests of cocoa (Theobroma cacao L.) using insecticides began in 1950 and has since gone through various programmes with concomitant challenges and successes. Presently Imidacloprid (Confidor®), Bifenthrin (Akatemaster®) and Thiamethoxam (Actara®)are recommended by Ghana Cocoa Board (COCOBOD) for the management of insect pests. A survey was conducted in the Ashanti, Eastern, Volta and Western Regions of Ghana using questionnaires and farm visits of 147 cocoa farmers’ fields to gather information on the characteristics of the farmers and insecticide handling and use by respondents. The survey showed that males dominated cocoa farming (72.7%) and most of them aged between 50 and 60 years. About 44% have had basic education whilst 37.5% of them belonged to farmer based organizations. About 52.8% of the farmers own motorized mistblower but 47.2% of the farmers use knapsack in the absence of a mistblower. About 44.8% do their own spray application whereas 55.2% hire labour. About 60.9% of the population across the regions read the label on the insecticides before application. A few (31.6 %) of the respondents put on the full personal protective costume during insecticide application and 21.9% do not use any protection. There was a positive correlation between farmers’ membership of farmer-based organisation and the costume-wearing farmers in the Ashanti, Eastern and Volta Regions and it was significant in the Ashanti region. It isrecommended that training and monitoring programmes be organized for farmers on the need to handle pesticides properly for personal and environmental safety and consumer benefit.Keywords: Insecticides; pest management; cocoa farmers; safet

Incipient bifenthrin-resistance in field populations of cocoa mirids, Distantiella theobroma (Dist.) and Sahlbergella singularis Hagl. (Hemiptera: Miridae)

Bifenthrin is one of the insecticides approved by the Ghana Cocoa Board (COCOBOD) for the control of cocoa mirids. Bifenthrin-resistance levels of D. theobroma and S. singularis collected from different locations in the Eastern Region (ER), Volta Region (VR) and Central Region (CR) were determined by calculating their resistance ratios (RRs). The highest RRs for S. singularis were recorded from New Tafo in the ER and Logba Alikpati in the VR, which displayed 6-fold and 6.2- fold bifenthrin-resistance, respectively. Populations of S. singularis from Pankese in the ER and Likpe in the VR also displayed 3.6-fold and 3.9-fold bifenthrin-resistance, respectively. The D. theobroma population that displayed the highest bifenthrin-resistance of 3.7-fold was from New Tafo. The results show incipient bifenthrin-resistance in field populations of D. theobroma and S. singularis, which requires the implementation of effective resistance management strategies to prevent full-blown resistance in the cocoa mirids

Evaluation of Eggplant, Solanum spp. Germplasm against Field Insect Pests’ Infestation at Bunso in the Eastern Region of Ghana

Eggplant (Solanum spp.) germplasm from CSIR – Plant Genetic Resources Research Institute, Bunso, Ghana were evaluated in field experiments against insect pests’ infestation during the major rainy seasons of 2009 and 2010. Twenty-six accessions were evaluated in 2009 and five were subjected to further evaluation in 2010. Insect species recorded on eggplant accessions in both years included Aphis gossypii (Glover), Thrips tabaci (Lindeman) and Leucinodes orbonalis (Gueneé). Camponatus sp. (carpenter ants) was also collected in 2009.  In both 2009 and 2010, leaves of accession GH 5171 harbored significantly lower numbers of A. gossypii.  Leaves of accessions GH 1208 and GH 1113 also harbored the least number of T. tabaci in 2009 and 2010, respectively. T. tabaci preferred flowers to leaves of all eggplant accessions evaluated in 2010. The population dynamics of T. tabaci also fluctuated less on eggplant flowers than on the leaves. The percentage stems attacked by L. orbonalis were not significantly different among accessions in both years.  With respect to fruit infestation by L. orbonalis, although fruits of accessions GH 1208, GH 3944 and GH 3947 were significantly (P < 0.001) less susceptible to infestation in 2009 their yields were relatively low.  Yield obtained ranged from 0 kg/ha in accession GH 1202 (2009) to 837.86 kg/ha in accession GH 5183 (2010). Accessions GH 1113 and GH 5171 combined a relatively good yield with moderate levels of tolerance to all insect pest species identified in this experiment and are consequently recommended for crop improvement programs. Keywords: Accession, Aphis gossypii, eggplant, Leucinodes orbonalis, Thrips tabac

Development and Evaluation of a Low-Cost Probe-Type Instrument to Measure the Equilibrium Moisture Content of Grain

Storage of grain in bags is common in Africa, Asia, and many other less developed countries making a bag probing method well-suited for moisture content (MC) measurement. A low-cost meter was developed under a USAID project to reduce post-harvest loss (PHL). The meter, referred to as the PHL meter, measures the MC of maize and other grains based on relative humidity (RH) and temperature (T) measurements obtained by a small digital sensor located in the tip of a tubular probe that can be inserted into bags of grain or other grain bulks. Measurements are used by equilibrium moisture content (EMC) equations programmed into the meter to predict MC. A handheld reader connected to the probe provides a user interface. The PHL moisture meter was evaluated based on laboratory studies in the United States and field studies in Ghana. Meter readings from field studies were compared to two commercial meters, a John Deere Chek-Plus-SW08120 grain moisture tester and a DICKEY-john GAC® 2100 Agri meter. The John Deere portable moisture meter is a low-cost meter by developed country standards (~US$250, 2016 price); the GAC 2100 bench-top moisture meter is an approved moisturetester by the Grain Inspection, Packers and Stockyards Administration (GIPSA) and has been a highly regarded and used electronic meter. Laboratory studies indicated that the PHL meter may require up to 6 min to take a measurement because of the time required by the probe tip and sensor to equilibrate to grain conditions. Methods to reduce the measurement time by measuring temporal equilibration rates were developed. These can be programmed into the reader to shorten measurement time for many conditions. The accuracy of the PHL moisture meter was comparable to the GAC 2100 moisture meter for maize below 15% MCwb. Average differences showed a positive offset of 0.45% for the PHL meter relative to the GAC 2100. The PHL meter provided an effective tool to probe bulk grain and bags

Evaluation of plastic and steel bins for protection of stored maize against Insect Infestation in Ghana: Poster

Maize is a staple food in Ghana where there is ever increasing demand for its use to also support poultry and livestock production. However, post-harvest loss of maize is high in Ghana. This study evaluated the effectiveness of plastic and steel bins as bulk storage structures to reduce maize post-harvest loss in Ejura, Ghana during the period from February 2016 to January 2017. Maize pre-disinfested with a solar biomass hybrid dryer was stored in the following treatments: i. a white 7-ton plastic bin filled with untreated maize, ii. agreen 7-ton plastic bin filled with untreated maize, iii.a 6-ton Kikapu steel bin filled with untreated maize, iv. six 50-kg polypropylene (PP) bags filled with maize treated with Betallic Super (80 g pirimiphos-methyl and 15 g permethrin per liter as an emulsifiable concentrate (EC)), and v. six 50-kg PP bags filled with untreated maize ascontrol. Moisture content, insect pests, insect damaged kernels (IDK), grain weight loss, aflatoxin and fumonisin levels data were collected monthly. Sitophilus zeamais, Tribolium castaneum, Cathartus quadricollis, and Cryptolestes ferrugineus were the dominant insect species collected from maize samples. At the end of 12 months of storage, % IDK in the control was >17% while IDK values in the other treatments were <3%. Mean grain weight losses of <1% were recorded in the bin treatments. Mycotoxin levels in the control were above the allowable threshold of 15 ppb. Our data suggest that use of plastic and steel bins has potential to reduce post-harvest loss of maize during storage.Maize is a staple food in Ghana where there is ever increasing demand for its use to also support poultry and livestock production. However, post-harvest loss of maize is high in Ghana. This study evaluated the effectiveness of plastic and steel bins as bulk storage structures to reduce maize post-harvest loss in Ejura, Ghana during the period from February 2016 to January 2017. Maize pre-disinfested with a solar biomass hybrid dryer was stored in the following treatments: i. a white 7-ton plastic bin filled with untreated maize, ii. agreen 7-ton plastic bin filled with untreated maize, iii.a 6-ton Kikapu steel bin filled with untreated maize, iv. six 50-kg polypropylene (PP) bags filled with maize treated with Betallic Super (80 g pirimiphos-methyl and 15 g permethrin per liter as an emulsifiable concentrate (EC)), and v. six 50-kg PP bags filled with untreated maize ascontrol. Moisture content, insect pests, insect damaged kernels (IDK), grain weight loss, aflatoxin and fumonisin levels data were collected monthly. Sitophilus zeamais, Tribolium castaneum, Cathartus quadricollis, and Cryptolestes ferrugineus were the dominant insect species collected from maize samples. At the end of 12 months of storage, % IDK in the control was >17% while IDK values in the other treatments were <3%. Mean grain weight losses of <1% were recorded in the bin treatments. Mycotoxin levels in the control were above the allowable threshold of 15 ppb. Our data suggest that use of plastic and steel bins has potential to reduce post-harvest loss of maize during storage