Signal amplification in electrochemical detection of buckwheat allergenic protein using field effect transistor biosensor by introduction of anionic surfactant

AbstractFood allergens, especially buckwheat proteins, sometimes induce anaphylactic shock in patients after ingestion. Development of a simple and rapid screening method based on a field effect transistor (FET) biosensor for food allergens in food facilities or products is in demand. In this study, we achieved the FET detection of a buckwheat allergenic protein (BWp16), which is not charged enough to be electrically detected by FET biosensors, by introducing additional negative charges from anionic surfactants to the target proteins. A change in the FET characteristics reflecting surface potential caused by the adsorption of target charged proteins was observed when the target sample was coupled with the anionic surfactant (sodium dodecyl sulfate; SDS), while no significant response was detected without any surfactant treatment. It was suggested that the surfactant conjugated with the protein could be useful for the charge amplification of the target proteins. The surface plasmon resonance analysis revealed that the SDS-coupled proteins were successfully captured by the receptors immobilized on the sensing surface. Additionally, we obtained the FET responses at various concentrations of BWp16 ranging from 1ng/mL to 10μg/mL. These results suggest that a signal amplification method for FET biosensing is useful for allergen detection in the food industry

ASK1-dependent recruitment and activation of macrophages induce hair growth in skin wounds

Apoptosis signal-regulating kinase 1 (ASK1) is a member of the mitogen-activated protein 3-kinase family that activates both c-Jun NH2-terminal kinase and p38 pathways in response to inflammatory cytokines and physicochemical stress. We report that ASK1 deficiency in mice results in dramatic retardation of wounding-induced hair regrowth in skin. Oligonucleotide microarray analysis revealed that expression of several chemotactic and activating factors for macrophages, as well as several macrophage-specific marker genes, was reduced in the skin wound area of ASK1-deficient mice. Intracutaneous transplantation of cytokine-activated bone marrow-derived macrophages strongly induced hair growth in both wild-type and ASK1-deficient mice. These findings indicate that ASK1 is required for wounding-induced infiltration and activation of macrophages, which play central roles in inflammation-dependent hair regrowth in skin

The Development and Assessment on the Social Studies Handbook for Supporting Teacher’s Lesson Planning and Improvement : A Content Structure of Handbook which can be Applied to Pre-service and In-service Teacher Education

The purposes of this paper are to develop the draft of handbook for planning, teaching and accessing the class of social studies and evaluate effects of the handbook for teacher training and their professional development. The structure of the first draft was designed based on Kolb’s learning theory. The present results suggested that the usefulness of the contents structure was perceived by (1) pre-service teachers and (2) in-service teachers, and the possibility for application was also recognized by the teacher educator as (3) university professor who teach methods courses, (4) senior supervisor who is in charge of designing the professional development programs and (5) younger supervisor who is in charge of tutoring the novice teacher, but they illustrated their different types of the significances, limits and utilization according to their purposes and as well as their responsibility. The authors implicated the alterative design of the handbook based on Korthagen’s reflective learning model for meeting their purposes and solving the structural problems inherit in the handbook

Origin of chiral discrimination by a two-dimensionally chiral self-assembled monolayer : A quantum chemical study

Structural and energetic investigations based on semiempirical PM5 and ab initio HF and MP2 calculations suggested that the self-assembled monolayer of an atropisomeric compound, (R)- or (S)-1,1′-binaphthalene-2,2′-dithiol (BNSH), on a gold (111) surface selectively adsorbs one enantiomer of phenylalanine (Phe), resulting in chiral discrimination, through hydrophobic, cation(–NH3+)-π and NH(Phe)-π(BNSH) interactions in a nanometer-sized screw-hole-like pocket composed of three BNSH molecules in the monolayer