Model evaluation and optimisation of nutrient removal potential for sequencing batch reactors

Performance of sequencing batch reactors for simultaneous nitrogen and phosphorus removal is evaluated by means of model simulation, using the activated sludge model, ASM2d, involving anoxic phosphorus uptake, recently proposed by the IAWQ Task group. The evaluation includes all major process configurations with different aerobic, anoxic, anaerobic sequences, and fill conditions. Basic relationships between modelling and design based on overall process stoichiometry are established for the interpretation of nutrient profiles associated with different operation modes. A similar approach is also used for the assessment of the effect of major operating parameters on system performance. WaterSA Vol.28(4) 2002: 423-43

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Challenges in microbial ecology: building predictive understanding of community function and dynamics.

The importance of microbial communities (MCs) cannot be overstated. MCs underpin the biogeochemical cycles of the earth's soil, oceans and the atmosphere, and perform ecosystem functions that impact plants, animals and humans. Yet our ability to predict and manage the function of these highly complex, dynamically changing communities is limited. Building predictive models that link MC composition to function is a key emerging challenge in microbial ecology. Here, we argue that addressing this challenge requires close coordination of experimental data collection and method development with mathematical model building. We discuss specific examples where model-experiment integration has already resulted in important insights into MC function and structure. We also highlight key research questions that still demand better integration of experiments and models. We argue that such integration is needed to achieve significant progress in our understanding of MC dynamics and function, and we make specific practical suggestions as to how this could be achieved

A NONCOMMUTATIVE-THEORY OF BADE FUNCTIONALS

Since the pioneering work of W. G. Bade [3, 4] a great deal of work has been done on bounded Boolean algebras of projections on a Banach space ([11, XVII.3.XVIII.3], [21, V.3], [16], [6], [12], [13], [14], ]17], [18], [23], [24]). Via the Stone representation space of the Boolean algebra, the theory can be studied through Banach modules over C(K), where K is a compact Hausdorff space. One of the key concepts in the theory is the notion of Bade functionals. If X is a Banach C(K)-module and x ε X, then a Bade functional of x with respect to C(K) is a continuous linear functional α on X such that, for each a in C(K) with a ≥ 0, we have (i) α (ax) ≥0, (ii) if α (ax) = 0, then ax = 0

COD fractionation based biological treatability assessment of segregated & recovered wastewater streams from denim processing plant

This study evaluated the biological treatability of overall wastewater generated from a denim processing plant located in Corlu, Tekirdag, in the NW of Turkey, before and after sub-stream wastewaters' recovery alternatives were performed in the plant processes. It was determined that there are 5 segregated sub-streams composed of 11 segregated single sub-stream wastewaters contribute overall wastewater of the plant. The plant overall wastewater reflects the typical character of denim processing effluents which have a high level of chemical oxygen demand (CODTot) 1200-1577 mg.l(-1) (1390 mg.l(-1)), CODSol 700-1210 mg.l(-1) (955 mg.l(-1)), TSS 2840-6800 mg.l(-1) (4820 mg.l(-1)) and quite acidic/neutral pH value such as 5.8-6.5 (6.2). According to the segregated stream characters, 6 segregated single sub-stream wastewaters fitted to be suitable for recovery. After recovery, it was observed that the remaining wastewater character changed as COD Tot 1750-2295 mg.l(-1)(2020 mg.l(-1)), CODSol 1080-1695 mg.l(-1) (1390 mg.l(-1)), TSS 3330-9735 mg.l(-1) (6535 mg.l(-1)) and pH value 5.3-6.5 (5.9). According to the biodegradability experimental data, the soluble inert fraction (S-I) of the initial total (COD) (S-I/C-T) remain steady by the ratio 3% before and after recovery while particulate inert fraction (X-I/C-T) increased from 1.6% to 3.4% ratio, as it can be considered as a specific case of COD fractions for recovery. But when the all biological treatability characteristics were evaluated as a private for this plant, it was observed that there is no any difficulty for biological treatability of remaining wastewater after recovery.Scientific Research Council of Namik Kemal UniversityNamik Kemal University [NKU BAP 00.17.YL.09.06]This paper was produced by associated with a Msc. Thesis titled as Determination of Inert COD Value of Denim Washing Wastewater After In plant Recovery & Reuse Alternative, funded by the Scientific Research Council of Namik Kemal University via Grant No. NKU BAP 00.17.YL.09.06. Thanks are due for the additional support provided by Environmental Engineering Department laboratory of Istanbul Technical University for the respirometric experimental work. We also thank the technical and administrative staff of the denim processing plant for their cooperation and assistance for this study