11 research outputs found
Effect of protamine sulfate on the isolated mesenteric arteries of normotensive and spontaneously hypertensive rats
We tested the relaxant effect of increased protamine sulfate (PS) amounts (10, 20, 50, 100 and 150 Ī¼g/ml) on the isolated mesenteric arteries of normotensive and spontaneously hypertensive (SH) rats, with or without endothelium. PS caused concentration-dependent relaxation of isolated mesenteric arteries in both types of rats. The relaxation effect of PS was lower in SH rats than in normotensive ones. Our results indicate that the vascular smooth muscles play a significant role in PS-mediated relaxation.Ispitivan je relaksantni efekat rastuÄih koliÄina (10, 20, 50, 100 i 150 Ī¼g/ml) protamin sulfata (PS) na izolovanim mezenteriÄnim arterijama normotenzivnih i spontano hipertenzivnih (SH) pacova sa i bez endotela. PS je uzrokovao koncentracijski zavisnu relaksaciju izolovanih mezenteriÄnih arterija kod oba tipa pacova. Kako je relaksantni efekat bio slabiji kod SH pacova uporeÄenju sa normotenzivnim, naÅ”i rezultati ukazuju da pored vaskularnog endotela, glatki miÅ”iÄi krvnih sudova imaju veoma znaÄajnu ulogu u PS-posredovanoj relaksaciji.Projekat ministarstva br. 143034B: The Role of Redox-Active Substances in the Maintenance of Homeostasi
Effect of protamine sulfate on the isolated mesenteric arteries of normotensive and spontaneously hypertensive rats
We tested the relaxant effect of increased protamine sulfate (PS) amounts (10, 20, 50, 100 and 150 Ī¼g/ml) on the isolated mesenteric arteries of normotensive and spontaneously hypertensive (SH) rats, with or without endothelium. PS caused concentration-dependent relaxation of isolated mesenteric arteries in both types of rats. The relaxation effect of PS was lower in SH rats than in normotensive ones. Our results indicate that the vascular smooth muscles play a significant role in PS-mediated relaxation.Ispitivan je relaksantni efekat rastuÄih koliÄina (10, 20, 50, 100 i 150 Ī¼g/ml) protamin sulfata (PS) na izolovanim mezenteriÄnim arterijama normotenzivnih i spontano hipertenzivnih (SH) pacova sa i bez endotela. PS je uzrokovao koncentracijski zavisnu relaksaciju izolovanih mezenteriÄnih arterija kod oba tipa pacova. Kako je relaksantni efekat bio slabiji kod SH pacova uporeÄenju sa normotenzivnim, naÅ”i rezultati ukazuju da pored vaskularnog endotela, glatki miÅ”iÄi krvnih sudova imaju veoma znaÄajnu ulogu u PS-posredovanoj relaksaciji.Projekat ministarstva br. 143034B: The Role of Redox-Active Substances in the Maintenance of Homeostasi
Influence of Radiation Dose in Computed Tomography on Antioxidant Enzyme Activity in Rabbit Erythrocytes
The objective of this study was to assess the radiation dose in computed tomography examinations of rabbits using different examination protocols and to correlate these values with the activity of antioxidant enzymes in their red blood cells following irradiation. The presented results revealed that a single, routine computed tomography scan exposure led to a different response of the activity of antioxidant enzymes in red blood cells regarding both dose and time. The results indicate that there is a dose threshold that is about 25 mGy. Doses below that level do not produce any significant changes in the level of antioxidant enzymes activity. On the other hand, the level just above that threshold had a significant impact on the antioxidant defence, but in a relatively short time period (2 hours after exposure), compared to the higher dose that requires a longer adaptive period
EFFECT OF PROTAMINE SULFATE ON THE ISOLATED MESENTERIC ARTERIES
the isolated mesenteric arteries of normotensive and spontaneously hypertensive (SH) rats, with or without endothelium. PS caused concentration-dependent relaxation of isolated mesenteric arteries in both types of rats. The relaxation effect of PS was lower in SH rats than in normotensive ones. Our results indicate that the vascular smooth muscles play a significant role in PS-mediated relaxation
Beyond H2S and NO Interplay: Hydrogen Sulfide and Nitroprusside React Directly to Give Nitroxyl (HNO). A New Pharmacological Source of HNO
Hydrogen sulfide (H2S) has been increasingly recognized as an important signaling molecule that regulates both blood pressure and neuronal activity. Attention has been drawn to its interactions with another gasotransmitter, nitric oxide (NO). Here, we provide evidence that the physiological effects observed upon the application of sodium nitroprusside (SNP) and H2S can be ascribed to the generation of nitroxyl (HNO), which is a direct product of the reaction between SNP and H2S, not a consequence of released NO subsequently reacting with H2S. Intracellular HNO formation has been confirmed, and the subsequent release of calcitonin gene related peptide from a mouse heart has been demonstrated. Unlike with other thiols, SNP reacts with H2S in the same way as rhodanese, i.e, the cyanide transforms into a thiocyanate. These findings shed new light on how H2S is understood to interact with nitroprusside. Additionally, they offer a new and convenient pharmacological source of HNO for therapeutic purposes.FAU Erlangen-Nuremberg (Emerging Field Initiative: Medicinal Redox Inorganic Chemistry); DAAD; Serbian Ministry of Education and science [OI 173014
Fullerenol C-60(OH)(24) nanoparticles decrease relaxing effects of dimethyl sulfoxide on rat uterus spontaneous contraction
Dimethyl sulfoxide (DMSO) is a widely used solvent and cryoprotectant that can cause impaired blood flow, reduction in intracranial pressure, tissue edema, inflammatory reactions, inhibition of vascular smooth muscle cell migration and proliferation, processes which can lead to atherosclerosis of the coronary, peripheral and cerebral circulation. Although the adverse effects are rare when DMSO is administered in clinically established concentrations, there is no safe antagonist for an overdose. In this work, we treated isolated spontaneous and calcium-induced contractile active rat uteri (Wistar, virgo intacta), with DMSO and fullerenol C-60(OH)(24) nanoparticle (FNP) in DMSO. FNP is a water-soluble derivative of fullerene C-60. Its size is a 1.1 nm in diameter and is a very promising candidate for a drug carrier in nanomedicine. FNP also displays free radical scavenging activity. DMSO decreased both spontaneous and calcium-induced contractions. In contrast, FNP only decreased spontaneous contraction. FNP decreased copper-zinc superoxide dismutase activity and prevented the DMSO-induced increase in glutathione reductase activity. Atomic force microscopy detected that FNP aggregated with calcium ions. Our results indicate that FNP has properties that make it a good candidate to be a modulator of DMSO activity which could minimize side effects of the latter
Influence of radiation dose in computed tomography on antioxidant enzyme activity in rabbit erythrocytes
The objective of this study was to assess the radiation dose in computed
tomography examinations of rabbits using different examination protocols and
to correlate these values with the activity of antioxidant enzymes in their
red blood cells following irradiation. The presented results revealed that a
single, routine computed tomography scan exposure led to a different response
of the activity of antioxidant enzymes in red blood cells regarding both dose
and time. The results indicate that there is a dose threshold that is about
25 mGy. Doses below that level do not produce any significant changes in the
level of antioxidant enzymes activity. On the other hand, the level just
above that threshold had a significant impact on the antioxidant defence, but
in a relatively short time period (2 hours after exposure), compared to the
higher dose that requires a longer adaptive period. [Project of the Serbian
Ministry of Education, Science and Technological Development, Grant no.
173014: Molecular mechanisms of redox signalling in homeostasis: adaptation
and pathology