The study of greening shotcrete considered the landscape

This study concerns the protection method and the natural maintenance of aging shotcrete. In Japan, in the high economic growth period in 1960’s, a great number of slopes were formed to construct many roads and most slope protection methods were to cover shotcrete on the slope. In recent years, with concerns about the environmental and landscape, the construction of shotcrete is decreasing. However, even now about 7,000,000㎡of shotcrete is laid every year, and it is predicted that the total amount of shotcrete is enormous. Now, about 60 years passes after a great number of shotcrete was laid, and shotcrete have been aging. Therefore, we suggested the method which construct for directly aging shotcrete in the protection method and the greening protection method is considering cost and risk with new construction. We adopt the protection method by rock bolt and rope net. In this study, at first, we establish the vegetation base suitable for growth of plants which can be use together with the protection method. And then, we install many kind of the vegetation base that gave the vegetation on a model shotcrete. Finally, we compared the rates of water retention and vegetation growth

Induction of Robust Immune Responses against Human Immunodeficiency Virus Is Supported by the Inherent Tropism of Adeno-Associated Virus Type 5 for Dendritic Cells

The ability of adeno-associated virus serotype 1 to 8 (AAV1 to AAV8) vectors expressing the human immunodeficiency virus type 1 (HIV-1) Env gp160 (AAV-HIV) to induce an immune response was evaluated in BALB/c mice. The AAV5 vector showed a higher tropism for both mouse and human dendritic cells (DCs) than did the AAV2 vector, whereas other AAV serotype vectors transduced DCs only poorly. AAV1, AAV5, AAV7, and AAV8 were more highly expressed in muscle cells than AAV2. An immunogenicity study of AAV serotypes indicates that AAV1, AAV5, AAV7, and AAV8 vectors expressing the Env gp160 gene induced higher HIV-specific humoral and cell-mediated immune responses than the AAV2 vector did, with the AAV5 vector producing the best responses. Furthermore, mice injected with DCs that had been transduced ex vivo with an AAV5 vector expressing the gp160 gene elicited higher HIV-specific cell-mediated immune responses than did DCs transduced with AAV1 and AAV2 vectors. We also found that AAV vectors produced by HEK293 cells and insect cells elicit similar levels of antigen-specific immune responses. These results demonstrate that the immunogenicity of AAV vectors depends on their tropism for both antigen-presenting cells (such as DCs) and non-antigen-presenting cells (such as muscular cells) and that AAV5 is a better vector than other AAV serotypes. These results may aid in the development of AAV-based vaccine and gene therapy