Protein encapsulation by electrospinning and electrospraying

Given the increasing interest in the use of peptide- and protein-based agents in therapeutic strategies, it is fundamental to develop delivery systems capable of preserving the biological activity of these molecules upon administration, and which can provide tuneable release profiles. Electrohydrodynamic (EHD) techniques, encompassing electrospinning and electrospraying, allow the generation of fibres and particles with high surface area-to-volume ratios, versatile architectures, and highly controllable release profiles. This review is focused on exploring the potential of different EHD methods (including blend, emulsion, and co−/multi-axial electrospinning and electrospraying) for the development of peptide and protein delivery systems. An overview of the principles of each technique is first presented, followed by a survey of the literature on the encapsulation of enzymes, growth factors, antibodies, hormones, and vaccine antigens using EHD approaches. The possibility for localised delivery using stimuli-responsive systems is also explored. Finally, the advantages and challenges with each EHD method are summarised, and the necessary steps for clinical translation and scaled-up production of electrospun and electrosprayed protein delivery systems are discussed

Farmers’ Adaptation Initiatives to the Impact of Climate Change on Agriculture in Northern Nigeria

The study ascertained the innovative adaptive measures used by farmers in northern Nigeria to cushion the harmful effects of climate change. Quantitative and qualitative approaches (Rapid Rural Appraisal, focus group discussions, and semi – structured interview schedule) were used to elicit information from a total of 500 farmers, which were selected using multistage random sampling technique. Findings revealed that 84% and 79% of the farmers were aware and knowledgeable of climate change issues, respectively, while 81% of them noted that they had at various times experienced climate change incidences. Farmers reported that factors which informed their perception of climate change incidence were: unusual early rains followed by weeks of dryness (M= 2.84), erratic rainfall pattern (M= 2.66), drought (M=2.68), reduction in farm yields (M=2.68) and high rate of disease incidence (M= 2.67). Adaptive measures used by the farmers in northern Nigeria included: changes in planting dates (88.4%) and harvesting dates (85.4%), multiple cropping (81.8%), intensive manure application (69.2%), shift to different sites (56.8%) and use of wetland/river valley (fadama) for farming (52.6%). The study concludes that there is need for government to make concrete efforts to enact appropriate policies on climate change adaptation and assist local farmers in the short and long term to improve their resilience to climate change impact

Farmers’ Adaptation Initiatives to the Impact of Climate Change on Agriculture in Northern Nigeria

The study ascertained the innovative adaptive measures used by farmers in northern Nigeria to cushion the harmful effects of climate change. Quantitative and qualitative approaches (Rapid Rural Appraisal, focus group discussions, and semi – structured interview schedule) were used to elicit information from a total of 500 farmers, which were selected using multistage random sampling technique. Findings revealed that 84% and 79% of the farmers were aware and knowledgeable of climate change issues, respectively, while 81% of them noted that they had at various times experienced climate change incidences. Farmers reported that factors which informed their perception of climate change incidence were: unusual early rains followed by weeks of dryness (M= 2.84), erratic rainfall pattern (M= 2.66), drought (M=2.68), reduction in farm yields (M=2.68) and high rate of disease incidence (M= 2.67). Adaptive measures used by the farmers in northern Nigeria included: changes in planting dates (88.4%) and harvesting dates (85.4%), multiple cropping (81.8%), intensive manure application (69.2%), shift to different sites (56.8%) and use of wetland/river valley (fadama) for farming (52.6%). The study concludes that there is need for government to make concrete efforts to enact appropriate policies on climate change adaptation and assist local farmers in the short and long term to improve their resilience to climate change impact

Injectables and Depots to Prolong Drug Action of Proteins and Peptides

Proteins and peptides have emerged in recent years to treat a wide range of multifaceted diseases such as cancer, diabetes and inflammation. The emergence of polypeptides has yielded advancements in the fields of biopharmaceutical production and formulation. Polypeptides often display poor pharmacokinetics, limited permeability across biological barriers, suboptimal biodistribution, and some proclivity for immunogenicity. Frequent administration of polypeptides is generally required to maintain adequate therapeutic levels, which can limit efficacy and compliance while increasing adverse reactions. Many strategies to increase the duration of action of therapeutic polypeptides have been described with many clinical products having been developed. This review describes approaches to optimise polypeptide delivery organised by the commonly used routes of administration. Future innovations in formulation may hold the key to the continued successful development of proteins and peptides with optimal clinical properties