In vitro Inhibitory Studies and Effect of Selected Plant Extracts and Cations on Elastase (EC 3.4.21.11) Activity produced by Aspergillus niger and Aspergillus flavus

Elastase is a member of the serine protease enzyme family that hydrolytically degrades elastin, a connective tissue component leading to aging and wrinkling of the skin. In this report, we investigated the in-vitro inhibitory effects of some cations and n-hexane extract of Hibiscus cannabinus, Vernonia amygdalina, Murraya koenigii, and Telfairia occidentalis on the activity of Elastase isolated from Aspergillus flavus and Aspergillus niger. Elastase was extracted, isolated and partially purified from Aspergillus flavus and Aspergillus niger. The data obtained in this study demonstrated that the activity of elastase in Aspergillus flavus was higher compared to that of Aspergillus niger after salting out. The elastase inhibition activities of Hibiscus cannabinus, Vernonia amygdalina, Murraya koenigii, and Telfairia occidentalis reveal that the leaf extract from Murraya koenigii, in Aspergillus flavus had more significant inhibition was obtained compared to that of Aspergillus niger, with inhibitory effect from 40µg/ml. While that of Hibiscus cannabinus showed similar inhibition characteristics to Murraya koenigii. Both Hibiscus cannabinus and Vernonia amygdalina had an effect at 80µg/ml; all the extracts appeared to have more effects on the enzyme obtained from Aspergillus flavus than from the Aspergillus niger. The potential of exploring these plant extract as an anti-aging recipe was discussed. Keywords: Inhibitory studies, Elastase, Aspergillus flavus, Aspergillus niger, Hibiscus cannabinus, Vernonia amygdalina, Murraya koenigii, and Telfairia occidentali

Anti-diabetic Properties and Phytochemical Studies of Ethanolic Leaf Extracts of Murraya Koenigii and Telfairia Occidentalis on Alloxan-Induced Diabetic Albino Rats

The antidiabetic properties and phytochemical studies of fractions of ethanolic leaf extract (400mg/Kgb.w) of Murraya koenigii (M. koenigii) and Telfairia occidentalis (T. occidentalis) was carried out on alloxan induced diabetic albino rats. Four and three fractions were obtained from M. koenigii and T. occidentalis extract respectively using column chromatography. Phytochemical screening of each fraction, indicate the presence of saponins, alkaloids, flavonoids, tannins and cardiac glycosides. The alloxan induced diabetic rats were treated with fractions of the extract, and fraction 1 and 2 of M. koenigii and T. occidentalis respectively which decreased blood glucose level significantly (p<0.05) by 72% and 78% respectively when compared within the group and showed no significant different when compared to normal control group. All treated groups showed no significant changes (p<0.05) in their body weight with the exception of groups treated with 3rd fractions of M. koenigii and T. occidentalis. Spectroscopic studies indicated the presence of  biological active compound in the 1st, 2nd and 3rd fractions of M. koenigii that absorbed maximally at 200-350nm and the 4th fraction showed absorption maximally at 270nm and 290nm; while the 1st fraction of T. occidentalis absorbed maximally at 320nm, 2nd fraction at 290nm and 340nm and 3rd fraction at 320nm and 350nm.  The 1st fraction of M. koenigii and 2nd fraction of T. occidentalis showed high antidiabetic properties at  λ max 320nm and 340nm respectively. Our findings certainly suggest among others the use of plants as a source of  potentially useful antidiabetic therapy for diabetics. Keywords: Diabetes mellitus, Antidiabetic components, Telfairia occidentalis and Murraya koenigi