Traditional Fermented Condiments Modulate Biochemical Indices in High Cholesterol Diet-Fed Rats

Hypercholesterolemia is implicated in cardiovascular diseases (CVD) and is identified as a common cause of mortality in this degenerative condition. Hence, CVD management strategy should include control of hypercholesterolemia. This study describes the effect of some legume condiments on biochemical indices in hypercholesterolemia rat model. Adult male Wistar rats were used for this study and hypercholesterolemia was induced by inclusion of 1% cholesterol in the rat feed. The animals were divided into five groups containing six animals each and were fed with diets supplemented with 16% fermented soybean, bambara groundnut and African locust bean. The study lasted for 30 days after which plasma was analyzed for the lipid profile and liver function marker enzymes and the liver tissue analyzed for malondialdehyde (MDA) content. Elevated plasma total cholesterol, triglyceride, LDL-cholesterol, alanine aminotransferase, aspartate transaminase, alkaline phosphatase and MDA content showed significant (p<0.05) reduction in the rats fed with fermented legume condiment-supplemented diets, with a concomitant increase in plasma HDL-cholesterol as compared with the hypercholesterolemia control rats. This study revealed that fermented legume condiment-supplemented diets attenuate hypercholesterolemia and protect the liver of the experimental rats from oxidative damage, with African locust bean condiment displaying the best biological potential

Modulatory effect of protocatechuic acid on cadmium induced nephrotoxicity and hepatoxicity in rats in vivo

Introduction: This study sought to investigate the effect of protocatechuic acid (PCA); a phenolic compound readily available in most plant foods on cadmium-induced nephrotoxicity and hepatoxicity in rats. Case description: Thirty six adult male rats weighing about 150–160 g were acclimatized for 2 weeks and subsequently divided into six groups: Group 1 rats received normal saline (control group), group 2 rats were administered 5 mg Cd/kg body weight in form of solution orally (induced group), groups 3 and 4 received cadmium solution and different doses of PCA (10 and 20 mg/kg body weight) respectively, while groups 5 and 6 were the normal rats administered different doses of PCA (10 and 20 mg/kg) respectively in an experiment that lasted for twenty one days. The animals were sacrificed, the blood was collected and the serum was subsequently prepared. Furthermore, the liver was excised, homogenized and centrifuged to obtain the tissue homogenate used for the analyses. The serum was used for the determination of the total protein, urea, creatinine and uric acid levels while the liver homogenate was used for the estimation of alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP). Discussion and evaluation: The result revealed that total protein level was reduced in cadmium induced toxicity rat group which was elevated upon treatment with PCA. Conversely, the elevated levels of urea, uric acid and creatinine in cadmium induced toxicity kidney rats were significantly (p < 0.05) reduced in PCA treated groups. Similarly, marked elevation in the ALT, AST and ALP activity were observed in cadmium induced toxicity rat group when compared with the control group. However, significant (p < 0.05) decrease in ALT, AST and ALP activity were noticed in groups administered different doses of PCA. Conclusions: The results from this study suggest that PCA may protect against cadmium-induced toxicity in the kidney and liver

Effects of drying on cholinesterases and angiotensin‐I converting enzyme inhibitory potential and phenolic constituents of African mistletoe (Loranthus bengwensis L) leaves from kolanut host tree

This study investigated the most appropriate drying method (sun drying, oven drying, and shade drying) for mistletoe leaves obtained from kolanut tree. The phenolic constituents were characterized using high performance liquid chromatography–diode array detector, while the inhibitory effect of the aqueous extracts of the leaves on cholinesterases and angiotensin‐I converting enzyme (ACE) and antioxidant activities were determined in vitro. The extracts inhibited acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and ACE in dose‐dependent manner. However, extract from sun‐dried sample exhibited the highest AChE, BChE, and ACE inhibitory effect while extract from shade‐dried sample had the least. Likewise, sun‐dried sample exhibited the highest antioxidant properties as exemplified by Fe2+‐chelating, 1,1‐diphenyl‐2 picrylhdrazyl, OH, and nitric oxide radical scavenging abilities. This study also revealed the presence of 20 phenolic compounds with caffeic acid being the most predominant. Conclusively, kolanut host tree mistletoe leaves can be used as therapeutic agent in the management of Alzheimer's disease and hypertension

Inhibition of α-amylase, α-glucosidase and oxidative stress by some common apple varieties

In recent times, the consumption of apples has been encouraged for the management of chronic diseases such as diabetes, but biochemical evidence to support this practice is lacking. Therefore, this study investigated α-amylase and α-glucosidase inhibitory activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability, Fe2+-induced lipid peroxidation potential as well as the total phenol and flavonoid contents of aqueous extracts of the apple varieties Malus sylvestris (green apple), Malus pumila (red apple) and Syzygium samarangense (wax apple). The results showed that all apple varieties inhibited α-amylase (IC50=12.66–16.98 µg/ml) and α-glucosidase (13.55–16.23 µg/ml) in a dosedependent manner, with green apple showing the highest inhibitory activity while wax apple had the least. Similarly, all apple varieties showed dose-dependent DPPH radical scavenging activity (EC50=222.92–278.71 µg/ml) with green apple also showing the highest scavenging activity while wax apple showed the least. Furthermore, the aqueous extracts of the apple varieties dosedependently inhibited Fe2+-induced lipid peroxidation in rat pancreas (38.60–53.57 µg/ml), with wax apple exhibiting the highest inhibitory potential. Also, the total phenol content of the apple varieties ranged from 16.14 to 17.45 mg GAE/100g, while the flavonoid content ranged from 4.17 to 5.56 mg QUE/100 g, with green apple having the highest total phenolic and flavonoid contents. The biological activities exhibited by the apple varieties could be attributed to the presence of biologically active photochemicals. Furthermore, the apple variety (green apple) with the highest phenolic content showed the best overall activity, indicating the potent role of phenolic compounds in the management of diabetes, thereby providing biochemical support for the use of apples as a functional food in diabetes management

Modulatory Effects of Ferulic Acid on Cadmium-Induced Brain Damage

Staff PublicationStudies have shown the pharmacological relevance of phenolics like ferulic acid (FA) in promoting health. This study sought to investigate the modulatory effects of FA on cadmium-induced brain damage in rats. Brain damage was induced in Wistar strain rats by oral administration of cadmium (5 mg/kg body weight) for 21 days. Assays for malondialdehyde (MDA) content, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), monoamine oxidase (MAO), and Naþ/Kþ-ATPase activities were carried out. The study revealed significant (P < .05) increases in the MDA content and all enzymes’ (AChE, BChE, MAO, and Naþ/Kþ- ATPase) activity investigated following cadmium administration. However, rats administered FA (10 and 20 mg/kg body weight) alongside cadmium significantly (P < .05) protected the brain by reversing the level of lipid peroxidation as measured by the MDA content as well as the enzymes’ activity. This study, therefore, substantiates the neuroprotective potentials of FA especially in the management of cadmium-induced toxicity

Comparative effect of quercetin and rutin on α-amylase, α-glucosidase, and some prooxidant-induced lipid peroxidation in rat pancreas

Staff PublicationThis study was designed to investigate the effect of quercetin, its glycosylated conjugate rutin and various combinations of the two flavonoids on carbohydrate-hydrolyzing enzymes associated with type 2 diabetes (α-amylase and α-glucosidase) in a bid to understand the possible mechanisms of action by which quercetin and rutin could be used in the prevention/management of the degenerative condition. The aim of this study is to investigate the effect of quercetin, rutin, and various combinations of the two flavonoids on α-amylase and α-glucosidase activities and the ability of the flavonoids to inhibit some pro-oxidants-induced lipid peroxidation in rat pancreas. The effect of the flavonoids on α-amylase and α-glucosidase activities as well as pro-oxidant (Fe2+ and SNP)-induced lipid peroxidation in rats’ pancreas homogenates was investigated. Rutin (IC50 = 0.048 μΜ) exhibited a significantly (P < 0.05) higher inhibition of Fe2+-induced lipid peroxidation than quercetin (IC50 = 0.075 μΜ). Furthermore, rutin had a stronger inhibition of α-amylase (IC50 = 0.043 μM) and α-glucosidase (IC50 = 0.037 μM) activities than quercetin [α-amylase (IC50 = 0.061 μM); α-glucosidase (IC50 0.038 μM)]. A combination of 25 % quercetin and 75 % rutin showed the strongest inhibition (P < 0.05) of α-amylase activity (IC50 = 313.87 μg/L) while 100 % quercetin showed the least inhibition of α-amylase (IC50 507.61 μg/L) activity and same trend was followed for α-glucosidase activity. This study revealed that glycosylation increased the inhibitory ability of quercetin on key enzymes linked to type 2 diabetes (α-amylase and α-glucosidase), and combination of quercetin and rutin had higher synergistic inhibitory abilities on the enzymes than the individual flavonoids, suggesting that a combination of food sources rich in these flavonoids could be very effective in the management/prevention of type 2 diabetes

In vitro antioxidant activities of African birch (Anogeissus leiocarpus) leaf and its effect on the α-amylase and α-glucosidase inhibitory properties of acarbose

Objective This study sought to determine the antioxidant activities of African birch leaf, to assess its interaction with key enzymes relevant to type 2 diabetes (α-amylase and α-glucosidase) and to evaluate its effect on acarbose in vitro. Methods One milligram per milliliter of aqueous extract of African birch and acarbose were separately prepared. At the same time, both the African extract and acarbose solution (50:50 v/v) were thoroughly mixed until homogeneity was attained. The phenolic phytoconstituents and antioxidant properties of African birch leaf were subsequently determined. Finally, the effects of African birch extract, acarbose solution and a mixture of acarbose and African birch extract on α-amylase and α-glucosidase activities were assessed in vitro. Results The results showed that African birch extract demonstrated a remarkable antioxidant effect, as exemplified by its radical scavenging abilities, Fe2+ chelating ability and prevention of lipid peroxidation. Acarbose had significantly (p < 0.05) higher α-amylase (IC50 = 11.77 μg/ml) and α-glucosidase (IC50 = 9.05 μg/ml) activities compared to African birch extract [α-amylase (IC50 = 242.17 μg/ml); α-glucosidase (IC50 = 196.35 μg/ml)]. However, the combination of acarbose and African birch extract showed an additive effect on α-amylase inhibition, while a resultant synergistic action was observed against α-glucosidase inhibition. Conclusion The additive and synergistic actions of the combination of African birch extract and acarbose solution suggest effective, complementary and alternative strategies towards the management/treatment of hyperglycaemia associated with type 2 diabetes

Anticholinesterase and Antioxidative Properties of Aqueous Extract of Cola acuminata Seed In Vitro

Staff PublicationBackground. Cola acuminata seed, a commonly used stimulant in Nigeria, has been reportedly used for the management of neurodegenerative diseases in folklore without scientific basis. This study sought to investigate the anticholinesterase and antioxidant properties of aqueous extracts from C. acuminata seed in vitro. Methodology. The aqueous extract of C. acuminata seed was prepared (w/v) and its effect on acetylcholinesterase (AChE) and butyrylcholinesterase activities, as well as some prooxidant (FeSO4, sodium nitroprusside (SNP), and quinolinic acid (QA)) induced lipid peroxidation in rat brain in vitro, was investigated. Results. The results revealed that C. acuminata seed extract inhibited AChE (IC50 = 14.6 μg/mL) and BChE (IC50 = 96.2 μg/mL) activities in a dose-dependent manner. Furthermore, incubation of rat’s brain homogenates with some prooxidants caused a significant increase in the brain malondialdehyde (MDA) content and inhibited MDA production dose-dependently and also exhibited further antioxidant properties as typified by their high radicals scavenging and Fe2+ chelating abilities. Conclusion. Inhibition of AChE and BChE activities has been the primary treatment method for mild Alzheimer’s disease (AD). Therefore, one possible mechanism through which the seed exerts its neuroprotective properties is by inhibiting cholinesterase activities as well as preventing oxidative-stress-induced neurodegeneration. However, this is a preliminary study with possible physiological implications

Impact of drying processes on Bryophyllum pinnatum phenolic constituents and its antiinflammatory and antioxidative activities in human erythrocytes

The effect of drying on the phytoconstituents, antioxidative, and anti-inflammatory properties of Bryophyllum pinnatum leaves was investigated. The phenolic constituents were characterized using HPLC-DAD. The aqueous extraction was done and various assays (Inhibition of membrane stabilization, albumin Denaturation and heatinduced hemolysis, malondialdehyde (MDA), and reduced glutathione (GSH) contents, as well as superoxide dismutase (SOD) activity), were carried out on human erythrocytes. The fresh portion (89.12 µg/ml) exhibited the highest potential to inhibit heat-induced hemolysis compared to the standard drug—Diclofenac (91.51 µg/ml). Freeze-dried sample showed the highest inhibitory potential on albumin denaturation ([Freeze-dried-330.72 µg/ml], [Diclofenac-318.63 µg/ml]) and membrane destabilization ([Freeze-dried-331.93 µg/ml], [Diclofenac-289.57 µg/ml]) when compared with Diclofenac. Similarly, the freeze-dried sample showed the highest GSH and SOD level and lowest MDA level when human erythrocytes challenged with tertiary butyl hydroperoxide (tBHP) were treated with the extract. This study confirms the retention of a considerable quantity of bioactive constituents of plants when freeze-dried

Effect of dietary supplementation of Padauk (Pterocarpus soyauxii) leaf on high fat diet/streptozotocin induced diabetes in rats’ brain and platelets

Background This study investigated the effects of Padauk leaf on brain malondialdehyde (MDA) content, acetylcholinesterase (AChE) activities, ectonucleotidases and adenosine deaminase (ADA) activities in the platelet of high fat diet and streptozotocin (STZ)-induced diabetic rats. Methods The animals were divided into six groups (n = 7): normal control rats; diabetic rats + high fat diet (HFD); diabetic rats + HFD + Metformin; diabetic rats + HFD + acarbose; diabetic rats + HFD + 10% Padauk leaf; normal rats + basal diet + 10% Padauk leaf. After 30 days of experiment comprising of acclimatization, dietary manipulation, pre-treatment with STZ and supplementation with Padauk leaf, the animals were sacrificed and the rats’ brain and blood were collected for subsequent analysis. Results The results demonstrated that the elevated MDA content and AChE activity in the diabetic rats were significantly reduced when compared with the control rats. Furthermore, the increased NTPDases, 5′-nucleotidase and ADA activities in the diabetic rats were significantly reduced when compared with the control rats. Conclusion This study demonstrated that Padauk leaf exhibited modulatory effects on purinergic and cholinergic enzymes involved in the prevention of platelet abnormality and consequent vascular complications in diabetic state