The First Case of Foot-and-Mouth Disease Caused by O/ME-SA/IND-2001 Virus in Kazakhstan

In Kazakhstan, foot-and-mouth disease (FMD) outbreaks were recorded in 2011–2013 in the southern and eastern regions where FMD vaccination is in place. In January 2022, in Kiikty village of the Shetsky district, Karaganda Region, Republic of Kazakhstan, cases among cattle clinically similar to foot-and-mouth disease were reported. The aim of the research was to establish the cause of the disease and to perform the typing of the pathogen. Materials and methods. Samples from sick animals were tested using PCR, sequenced and typed in compliance with the protocol developed by the International Office for Epizootic Diseases (OIE). Results and discussion. A cytopathogenic agent has been isolated from the BHK-21 cell culture, identified through real-time RT-PCR and electron microscopically as FMD virus. Molecular genetic studies have revealed that the infection of cattle in the Karaganda Region was caused by the FMD virus circulating in the territory of the countries bordering Kazakhstan (China, Russia, Mongolia) and belonging to type O, topotype ME-SA, genetic line Ind-2001. FMD virus isolated in Kazakhstan is genetically related to the FMD line from Asia and is a new FMDV genotype for Kazakhstan, assigned to the ME-SA topotype of the Ind-2001 genetic line. This study once again proves the need for continuous genetic typing of FMD viruses to improve the effectiveness of preventive measures

Сопоставление рентгенологической и патоморфологической картины легких у пациентов с COVID-19

Aim. Compare radiological patterns of COVID-19 pneumonia with pulmonary histology in deceased patients.Materials and methods. The analysis of recent lifetime CT studies of deceased patients was performed with the identification of all existing and leading CT symptoms, including “ground glass”, “crazy paving”, consolidation, as well as the symptom complex (pattern) of organizing pneumonia. Based on the CT symptoms, we selected the target points for taking the specimens by 3-D reconstructions. At the autopsy the lungs were entirely fixed into the front and then marked on CT sections cut from 1 to 3 pieces that were placed in paraffin and processed according to the standard technique, stained with hematoxylin and eosin and fuchsin-facelina. The specimens were analyzed by identifying all available histology changes and selecting the leading one.Results. 45 targeted pieces of lung tissue were obtained from 14 deceased COVID-19 patients (7 men/ 7 women), with an average age of 77.1 ± 12.9 (49–90 years). In deceased patients with the presence of the "ground glass" symptom, in most cases (57.1%) revealed an increase in intra-alveolar cellularity, hyaline membranes, desquamation of the alveolar epithelium and infiltration of the interalveolar septum by lymphocytes, which corresponds to the exudative phase of diffuse alveolar damage (DAP). Mosaic histological changes with alternation of filled alveoli (intraalveolar edema, clusters of red blood cells, macrophages, lymphocytes) and air alveoli were detected from the areas of “crazy paving” zones. Several cases demonstrated interstitial edema and lymphoid infiltration of interalveolar partitions of different severity without their thickening. Areas of consolidation were histologically represented by extensive intraalveolar hemorrhages and / or typical zones of hemorrhagic infarcts in 45.5% of cases. Perilobular consolidation, subpleural cords, symptoms of “halo” and “reverse halo”, which we considered as part of the symptom complex of organizing pneumonia in 43% of cases, morphologically corresponded to organizing pneumonia (the proliferative phase of DAP), as well as to distelectases.Conclusion. Comparison of CT patters and post-mortem pulmonary histology in COVID-19 deceased patients demonstrated that CT symptoms and patterns correspond to certain morphological changes of different phases of DAP.Цель исследования: сопоставить рентгенологические паттерны COVID-19 с гистологическими изменениями у умерших.Материал и методы. Проведен анализ последних прижизненных КТ-исследований умерших пациентов с выделением всех имеющихся и ведущего КТ-симптомов, включая “матовое стекло”, “булыжная мостовая”, консолидация, а также симптомокомплекс (паттерн) организующейся пневмонии. На основании выделенных КТ-симптомов были выбраны прицельные точки взятия материала при помощи построения трехмерных реконструкций. На аутопсии фиксированные целиком легкие разрезались фронтально, далее из обозначенных на компьютерной томограмме участков вырезали от 1 до 3 кусочков, которые заливались в парафин и обрабатывались по общепринятой методике с последующей окраской срезов толщи- ной 3–5 мкм гематоксилином и эозином, пикрофуксин-фукселином. Анализ материала проводили путем выявления всех имеющихся патоморфологических изменений с выделением ведущего из них.Результаты. Были получены 45 прицельно взятых кусочков ткани легкого от 14 умерших (7 мужчин/ 7 женщин), средний возраст 77,1 ± 12,9 (49–90) года. У умерших пациентов с наличием симптома “матово- го стекла” при КТ в большинстве случаев (57,1%) были выявлены увеличение числа клеток в просветах альвеол (внутриальвеолярная клеточность), гиалиновые мембраны, десквамация альвеолярного эпителия и инфильтрация лимфоцитами межальвеолярных перегородок, что может соответствовать признакам экссудативной фазы диффузного альвеолярного повреждения (ДАП). Из участков, обозначенных как зоны “булыжной мостовой”, были выявлены мозаичные гистологические изменения с чередованием заполненных альвеол (внутриальвеолярный отек, скопления эритроцитов, макрофагов, лимфоцитов) и воздушных альвеол, местами при наличии интерстициального отека и лимфоидной инфильтрации межальвеолярных перегородок разной степени выраженности без их утолщения. Участки консолидации гистологически были представлены обширными внутриальвеолярными кровоизлияниями и/или типичными зонами геморрагических инфарктов в 45,5% случаев. Перилобулярная консолидация, субплевральные тяжи, симптомы “ободка” и “обратного ободка”, которые мы расценивали в рамках симптомокомплекса организующейся пневмонии, на компьютерной томограмме в 43% случаев морфологически соответствовали организующейся пневмонии (пролиферативная фаза ДАП), а также дистелектазам.Заключение. При попытке рентгенопатоморфологического сопоставления у пациентов с COVID-19 с поражением легких нами было показано, что различные симптомы и паттерны при КТ соответствуют определенным морфологическим изменениям в различные фазы ДАП

Clinical features of post-COVID-19 period. Results of the international register “Dynamic analysis of comorbidities in SARS-CoV-2 survivors (AKTIV SARS-CoV-2)”. Data from 6-month follow-up

Aim. To study the clinical course specifics of coronavirus disease 2019 (COVID-19) and comorbid conditions in COVID-19 survivors 3, 6, 12 months after recovery in the Eurasian region according to the AKTIV register. Material and methods.The AKTIV register was created at the initiative of the Eurasian Association of Therapists. The AKTIV register is divided into 2 parts: AKTIV 1 and AKTIV 2. The AKTIV 1 register currently includes 6300 patients, while in AKTIV 2 — 2770. Patients diagnosed with COVID-19 receiving in- and outpatient treatment have been anonymously included on the registry. The following 7 countries participated in the register: Russian Federation, Republic of Armenia, Republic of Belarus, Republic of Kazakhstan, Kyrgyz Republic, Republic of Moldova, Republic of Uzbekistan. This closed multicenter register with two nonoverlapping branches (in- and outpatient branch) provides 6 visits: 3 in-person visits during the acute period and 3 telephone calls after 3, 6, 12 months. Subject recruitment lasted from June 29, 2020 to October 29, 2020. Register will end on October 29, 2022. A total of 9 fragmentary analyzes of the registry data are planned. This fragment of the study presents the results of the post-hospitalization period in COVID-19 survivors after 3 and 6 months. Results. According to the AKTIV register, patients after COVID-19 are characterized by long-term persistent symptoms and frequent seeking for unscheduled medical care, including rehospitalizations. The most common causes of unplanned medical care are uncontrolled hypertension (HTN) and chronic coronary artery disease (CAD) and/or decompensated type 2 diabetes (T2D). During 3- and 6-month follow-up after hospitalization, 5,6% and 6,4% of patients were diagnosed with other diseases, which were more often presented by HTN, T2D, and CAD. The mortality rate of patients in the post-hospitalization period was 1,9% in the first 3 months and 0,2% for 4-6 months. The highest mortality rate was observed in the first 3 months in the group of patients with class II-IV heart failure, as well as in patients with cardiovascular diseases and cancer. In the pattern of death causes in the post-hospitalization period, following cardiovascular causes prevailed (31,8%): acute coronary syndrome, stroke, acute heart failure. Conclusion. According to the AKTIV register, the health status of patients after COVID-19 in a serious challenge for healthcare system, which requires planning adequate health system capacity to provide care to patients with COVID-19 in both acute and post-hospitalization period

Biological features of common persimmon (Diospyros virginiana L.)

The article presents the results of long-term observations on the biology of common persimmon. Both positive and negative properties of this specie are given. Phenological indicators are provided: bud swelling, appearance of the first leaves, budding, flowering, fruit ripening. Diospyros virginiana L. is a hexaploid (2n = 90), however, it should be noted that along with the hexaploid race there is also a tetraploid one (2n = 60). Plants of this species are mainly dioecious, the flowers are bisexual. The flowering phase takes place in the first decade of June, which is one to two weeks later than that of the Eastern and Caucasian persimmons. The percentage of useful ovary is quite high and makes 27-29%. The fruit is smaller in size than the eastern persimmon, but three to four times larger than the Caucasian one. They have a spherical or conical shape, ranging in size from two to five cm in diameter. In a solid state, the fruits are very tart, but ripe fruits are very sweet, the sugar content reaches 20%. The ripening period is one and a half months. The number of seeds in the fruits is three to five pieces, they are larger in comparison with the seeds of the Caucasian persimmon. Diospyros virginiana L. is mainly found in the wild in the USA, where it is used as a rootstock on heavy, swampy soils for cultivated varieties of eastern persimmon, as it easily tolerates the proximity of groundwater, temporary flooding and river floods. In the Russian Federation, it is practically not used, since it is inferior in yield to plants grafted on Caucasian persimmon and forms a lot of growth. A great advantage of the common persimmon is its winter hardiness, it is much higher than that of cultivated varieties. It tolerates a drop in temperature to minus 25–280 С without any damage. In breeding, it is used to obtain varieties with high frost resistance to expand the cultivation area and move to more northern regions

Architectonics of the root system of Japanese persimmon depending on rootstock and relief

The article presents the long-term results of excavations of the root system of 10-year-old Japanese persimmon plants (‘Hiakume’ variety), grafted on two rootstocks (Oriental persimmon and common persimmon). It has been established that biological characteristics of rootstocks, topography, and tillage in the aisles affect significantly the total mass of the roots, as well as its distribution in the soil layers. Plants grafted on Oriental persimmon have a more developed and deeply penetrating root system. It is noted that on the slopes the development of the root system is uneven. The bulk of the roots is directed up the slope (72%), so a larger amount of mineral fertilizers must be applied to the upper terrace. On a flat site, the development of the root system occurs evenly around the crown of the plant, the roots are more powerful than those growing on the slope. Differences in remoteness from the stem have not been noted; in both cases, the roots have been placed at a distance of up to 250 cm in the aisle. As a result of the research, a direct relationship between the growth of the aerial part and the development of the root system has been determined, the stronger the aerial, the more powerful the root system

Selection results on subtropic fruit crops in the Russian Federation

The article presents the data of breeding studies on some subtropical fruit crops (Diospyros kaki L., Feijoa sellowiana Berg) cultivated in the Black Sea coast area of the Russian Federation. The first domestic ‘Khostinsky’ persimmon variety has been obtained, which has high productivity and quality of fruits, and is also resistant to pests and diseases. As a result of interspecific crossbreeding, a promising interspecific hybrid No. 99 (Omarov’s ISH) has been obtained (Djiro ’x common persimmon), which is highly frost-resistant can withstand temperature drops to minus 230.  Creation of such hybrids will expand the distribution area of the culture in more northern regions. The amount of sugars in fruits reaches 25% compared with 14-19 % in the control. As a result of multi-year research on feijoa plants cultivated in the humid subtropics of the Russian Federation, many promising forms have been identified among seed populations, three of them («Dagomysskaya», «Dachnaya», «Sentyabrskaya») were cultivated and included in the State Register of protected breeding achievements (plant varieties) of the RF in 2016. Selection of high-yield and especially early ripe forms of feijoa with their further vegetative propagation will increase significantly the area under the culture in the Russian Federation, and will increase the yield of plantations and improve the quality of fruits

Characteristics of japanese persimmon varieties cultivated in wet subtropics according to the ecological plasticity and stability parameters

The combination of high potential productivity and resistance to abiotic stressors is considered to be the main priority and criterion for evaluating Japanese Persimmon varieties. All varieties of Japanese persimmon grown in humid subtropics can be divided into intensive, plastic, low ecological plasticity and homeostatic ones - not responsive to environmental changes on the basis of the analysis conducted to assess the parameters of environmental plasticity and stability, depending on the conditions of a year

The Comparison Analysis of Orthophosphoric and Maleic Acids in the Smear Layer Removal Techniques

Different solutions have been used to remove the smear layer during endodontic treatment. Their efficacy, biological response and side effects differ from each other. The purpose of this review article is to assess systematically the available scientific evidence about the effectiveness of orthophosphoric and maleic acids in smear layer removing. The study of publications was produced in the electronic databases such as Google Scholar, PubMed during a systematic review of the literature. Included articles contain information about using orthophosphoric and maleic acids in smear layer removing and their difference. The publication date criterion was selected from January 2011 to September 2021. 55 articles were viewed during the review. After analyzing the literature for inclusion criteria, the total number of publications has become 10. According to literature data, orthophosphoric and maleic acids are both effective in removing the smear layer, however, they have some differences © 2022. Journal of International Dental and Medical Research.All Rights Reserved