Mars simulated exposure and the characteristic Raman biosignatures of amino acids and halophilic microbes

Though Raman bands of α-amino acids (AA) are well documented, often only the strongest intensity bands are quoted as identifiers (e.g. Jenkins et al., 2005; De Gelder et al., 2007; Zhu et al., 2011). Unknown regolith mixtures on Mars-sampling missions could obscure these bands. Here the case is made for determining, via a statistical method, sets of characteristic bands to be used as identifiers, independent of band intensity or number of bands (Rolfe et al., 2016). AA have upwards of 25 potentially identifying bands and this method defines sets of 10–19 bands per AA. Examination of AA-doped Mars-like basalt resulted in a maximum of eight bands being identified, as some characteristic bands were obscured by mineral bands, including the strongest intensity band in some cases. This proved the need for characteristic bands to be defined, enabling successful identification of AA. The ESA ExoMars Rover mission will crush and then pass the sample to the Raman Laser Spectrometer. We crushed a Mars-like basalt to a similar grain size expected to be created by the rover. Our samples were doped with 1 % (by weight) AA samples, resulting in no detection of AA, because of loss of original spatial context and spaces between the grains. We recommend that Raman spectroscopy on future missions should be conducted before the sample is crushed. Halite-entombed halophilic microbes, known to survive being entombed, were exposed to Mars-like surface (including temperature, pressure, atmospheric composition and UV) and freeze-thaw cycle (plus pressure and atmospheric composition) conditions. This test on the survival of the microbes showed that survival rates quickly deteriorated in surface conditions, but freeze-thaw cycle samples had well preserved Raman biosignatures, indicating that similar signatures could be detectable on Mars if similar life persists in evaporitic material or brines today

Critical Behavior of the Meissner Transition in the Lattice London Superconductor

We carry out Monte Carlo simulations of the three dimensional (3D) lattice London superconductor in zero applied magnetic field, making a detailed finite size scaling analysis of the Meissner transition. We find that the magnetic penetration length \lambda, and the correlation length \xi, scale as \lambda ~ \xi ~ |t|^{-\nu}, with \nu = 0.66 \pm 0.03, consistent with ordinary 3D XY universality, \nu_XY ~ 2/3. Our results confirm the anomalous scaling dimension of magnetic field correlations at T_c.Comment: 4 pages, 5 ps figure

Analytic Quantization of the QCD String

We perform an analytic semi-classical quantization of the straight QCD string with one end fixed and a massless quark on the other, in the limits of orbital and radial dominant motion. We compare our results to the exact numerical semi-classical quantization. We observe that the numerical semi-classical quantization agrees well with our exact numerical canonical quantization.Comment: RevTeX, 10 pages, 9 figure

Semileptonic form factors - a model-independent approach

We demonstrate that the B->D(*) l nu form factors can be accurately predicted given the slope parameter rho^2 of the Isgur-Wise function. Only weak assumptions, consistent with lattice results, on the wavefunction for the light degrees of freedom are required to establish this result. We observe that the QCD and 1/m_Q corrections can be systematically represented by an effective Isgur-Wise function of shifted slope. This greatly simplifies the analysis of semileptonic B decay. We also investigate what the available semileptonic data can tell us about lattice QCD and Heavy Quark Effective Theory. A rigorous identity relating the form factor slope difference rho_D^2-rho_A1^2 to a combination of form factor intercepts is found. The identity provides a means of checking theoretically evaluated intercepts with experiment.Comment: 18 pages, Revtex, 4 postscript figures, uses epsfig.st

Semi-leptonic B decays into higher charmed resonances

We apply HQET to semi-leptonic $B$ meson decays into a variety of excited charm states. Using three realistic meson models with fermionic light degrees of freedom, we examine the extent that the sum of exclusive single charmed states account for the inclusive semi-leptonic $B$ decay rate. The consistency of form factors with the Bjorken and Voloshin sum rules is also investigated.Comment: Latex, 27 pages. A few references and errors corrected, to appear in Phys. Rev.

From scalar to string confinement

We outline a connection between scalar quark confinement, a phenomenologically successful concept heretofore lacking fundamental justification, and QCD. Although scalar confinement does not follow from QCD, there is an interesting and close relationship between them. We develop a simple model intermediate between scalar confinement and the QCD string for illustrative purposes. Finally, we find the bound state masses of scalar, time-component vector, and string confinement analytically through semi-classical quantization.Comment: ReVTeX, 9 pages, 5 figure

Polarization observables of the gamma d --> PiNN reaction in the Delta(1232)-resonance region

Polarization observables of the three charge states of the pion for the $\gamma d\to\pi NN$ reaction with polarized photon beam and/or oriented deuteron target are evaluated over the whole $\Delta$(1232)-resonance region adopting a nonrelativistic model based on time-ordered perturbation theory. Results for the $\pi$-meson spectra, linear photon asymmetry, vector and tensor target asymmetries are presented. Particular attention is given, for the first time, to double polarization asymmetries for which we present results for $T_{20}^{\ell}$ and $T_{2\pm 2}^{\ell}$. We found that all other double polarization asymmetries of photon and deuteron target are vanished.Comment: 17 Pages, 8 Figures, accepted for publication in Int. J. Mod. Phys.

The P_33(1232) resonance contribution into the amplitudes M_{1+}^{3/2},E_{1+}^{3/2},S_{1+}^{3/2} from an analysis of the p(e,e'p)\pi^0 data at Q^2 = 2.8, 3.2, and 4 (GeV/c)^2 within dispersion relation approach

Within the fixed-t dispersion relation approach we have analysed the TJNAF and DESY data on the exclusive p(e,e'p)\pi^0 reaction in order to find the P_{33}(1232) resonance contribution into the multipole amplitudes M_{1+}^{3/2},E_{1+}^{3/2},S_{1+}^{3/2}. As an input for the resonance and nonresonance contributions into these amplitudes the earlier obtained solutions of the integral equations which follow from dispersion relations are used. The obtained values of the ratio E2/M1 for the \gamma^* N \to P_{33}(1232) transition are: 0.039\pm 0.029, 0.121\pm 0.032, 0.04\pm 0.031 for Q^2= 2.8, 3.2, and 4 (GeV/c)^2, respectively. The comparison with the data at low Q^2 shows that there is no evidence for the presence of the visible pQCD contribution into the transition \gamma N \to P_{33}(1232) at Q^2=3-4 GeV^2. The ratio S_{1+}^{3/2}/M_{1+}^{3/2} for the resonance parts of multipoles is: -0.049\pm 0.029, -0.099\pm 0.041, -0.085\pm 0.021 for Q^2= 2.8, 3.2, and 4 (GeV/c)^2, respectively. Our results for the transverse form factor G_T(Q^2) of the \gamma^* N \to P_{33}(1232) transition are lower than the values obtained from the inclusive data. With increasing Q^2, Q^4G_T(Q^2) decreases, so there is no evidence for the presence of the pQCD contribution here too

Pion photoproduction on nucleons in a covariant hadron-exchange model

We present a relativistic dynamical model of pion photoproduction on the nucleon in the resonance region. It offers several advances over the existing approaches. The model is obtained by extending our $\pi N$-scattering description to the electromagnetic channels. The resulting photopion amplitude is thus unitary in the $\pi N$, \ga N channel space, Watson's theorem is exactly satisfied. At this stage we have included the pion, nucleon, \De(1232)-resonance degrees of freedom. The $\rho$ and $\omega$ meson exchanges are also included, but play a minor role in the considered energy domain (up to $\sqrt{s}=1.5$ GeV). In this energy range the model provides a good description of all the important multipoles. We have allowed for only two free parameters -- the photocouplings of the $\Delta$-resonance. These couplings are adjusted to reproduce the strength of corresponding resonant-multipoles $M_{1+}$ and $E_{1+}$ at the resonance position.Comment: 17 pages, 4 figs, version to appear in Phys. Rev. C 70 (2004

Defining Multiple Characteristic Raman Bands of α-Amino Acids as Biomarkers for Planetary Missions Using a Statistical Method

Biomarker molecules, such as amino acids, are key to discovering whether life exists elsewhere in the Solar System. Raman spectroscopy, a technique capable of detecting biomarkers, will be on board future planetary missions including the ExoMars rover. Generally, the position of the strongest band in the spectra of amino acids is reported as the identifying band. However, for an unknown sample, it is desirable to define multiple characteristic bands for molecules to avoid any ambiguous identification. To date, there has been no definition of multiple characteristic bands for amino acids of interest to astrobiology. This study examinedL-alanine, L-aspartic acid, L-cysteine, L-glutamine and glycine and defined several Raman bands per molecule for reference as characteristic identifiers. Per amino acid, 240 spectra were recorded and compared using established statistical tests including ANOVA. The number of characteristic bands defined were 10, 12, 12, 14 and 19 for L-alanine (strongest intensity band: 832 cm-1), L-aspartic acid (938 cm-1), L-cysteine (679 cm-1),L-glutamine (1090 cm−1) and glycine (875 cm-1), respectively. The intensity of bands differed by up to six times when several points on the crystal sample were rotated through 360 °; to reduce this effect when defining characteristic bands for other molecules, we find that spectra should be recorded at a statistically significant number of points per sample to remove the effect of sample rotation. It is crucial that sets of characteristic Raman bands are defined for biomarkers that are targets for future planetary missions to ensure a positive identification can be made