Influence of a Single Dose of Meloxicam Administrated during Canine Estrus on Progesterone Concentration and Fertility—A Clinical Case Study

This case report presents an observation of the influence of meloxicam administration, during the periovulatory phase, on corpora lutea function in the domestic dog (Canis familiaris). A 2 year old female German Shepherd dog, with a level of progesterone suggestive of the periovulatory period (5.97 ng/mL), received a single subcutaneous injection of meloxicam (0.2 mg/kg body weight). In this female, subsequent evaluation to detect the optimal time for mating revealed a strong decrease in P4 (2.77 ng/mL), although it increased to 13.98 ng/mL within the following three days. The same female conceived and delivered a litter of the same size as in a subsequent cycle where meloxicam was not administered. The use of NSAID (nonsteroidal anti-inflammatory drugs), and particularly meloxicam, as a COX-2 inhibitor that influences the production of prostaglandins has been reported as being potentially harmful for ovulation and pregnancy in various species, including humans. In dogs, the secretion of prostaglandin E (PGE) is thought to be important for ovulation and the formation of the corpora lutea, the only source of P4, which is required for pregnancy maintenance. Although previous case reports have indicated an influence of meloxicam administration on CL function in domestic dogs, here, the decreased progesterone level observed directly after a single dose of meloxicam was only temporary, with no negative impact on fertility. Based on our observations, further studies related to the influence of the timing of meloxicam administration on ovarian cycle advancement, as well as the influence of duration of treatment, dosage, and route of administration on progesterone levels, as an indicator of CL function, are required

Dead or Alive? A Review of Perinatal Factors That Determine Canine Neonatal Viability

The perinatal period has a critical impact on viability of the newborns. The variety of factors that can potentially affect the health of a litter during pregnancy, birth, and the first weeks of life requires proper attention from both the breeder and the veterinarian. The health status of puppies can be influenced by various maternal factors, including breed characteristics, anatomy, quality of nutrition, delivery assistance, neonatal care, and environmental or infectious agents encountered during pregnancy. Regular examinations and pregnancy monitoring are key tools for early detection of signals that can indicate disorders even before clinical signs occur. Early detection significantly increases the chances of puppies’ survival and proper development. The purpose of the review was to summarize and discuss the complex interactions between all elements that, throughout pregnancy and the first days of life, have a tangible impact on the subsequent fate of the offspring. Many of these components continue to pose challenges in veterinary neonatology; thus, publications presenting the current state of knowledge in this field are in demand

Supporting the Wound Healing Process—Curcumin, Resveratrol and Baicalin in In Vitro Wound Healing Studies

The purpose of the investigation was to evaluate the effect of the selected bioflavonoids curcumin, resveratrol and baicalin on the wound healing process in an in vitro model. In the study, Balb3t3 and L929 cell lines were used. The first step was the evaluation of the cytotoxicity of the substances tested (MTT assay). Then, using the scratch test (ST), the influence of bioflavonoids on the healing process was evaluated in an in vitro model. The second stage of the work was a mathematical analysis of the results obtained. On the basis of experimental data, the parameters of the Brian and Cousens model were determined in order to determine the maximum value of the cellular and metabolic response that occurs for the examined range of concentrations of selected bioflavonoids. In the MTT assays, no cytotoxic effect of curcumin, resveratrol and baicalin was observed in selected concentrations, while in the ST tests for selected substances, a stimulatory effect was observed on the cell division rate regardless of the cell lines tested. The results obtained encourage further research on the use of substances of natural origin to support the wound healing process