The effect of surfactant chain length on the morphology of poly(methyl methacrylate) microcapsules for fragrance oil encapsulation

The solvent evaporation method for producing microcapsules relies upon the correct wetting conditions between the three phases involved in the synthesis to allow core-shell morphologies to form. By measuring the interfacial tensions between the oil, polymer and aqueous phases, spreading coefficients can be calculated, allowing the capsule morphology to be predicted. In this work we explore the effect of surfactant chain length on capsule morphology using poly(methyl methacrylate) as the polymer and hexadecane as the core. We compared the predicted morphologies obtained using the polymer as a solid, and the polymer dissolved in dichloromethane to represent the point at which capsule formation begins. We found that using the polymer in its final, solid form gave predictions which were more consistent with our observations. The method was applied to successfully predict the capsule morphologies obtained when commercial fragrance oils were encapsulated

Preparation and characterisation of anisotropic particles and microstructures

EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Validation of inhomogeneous Magnetization Transfer (ihMT) as a myelin biomarker

International audienceThe ihMT (inhomogeneous Magnetization transfer) signal qualitatively demonstrates sensitivity for myelinated structures. However, image intensity and contrast vary with the saturation parameters, further questioning the specificity of ihM for myelin. This study validated ihMT as a myelin biomarker by demonstrating for various ihMT sequences, linear relationships between ihMT signal and intensity of fluorescence microscopy (a quantitative myelin specific histology technique) collected in plp-GFP mouse brain

Validation of inhomogeneous Magnetization Transfer (ihMT) as a myelin biomarker

International audienceThe ihMT (inhomogeneous Magnetization transfer) signal qualitatively demonstrates sensitivity for myelinated structures. However, image intensity and contrast vary with the saturation parameters, further questioning the specificity of ihM for myelin. This study validated ihMT as a myelin biomarker by demonstrating for various ihMT sequences, linear relationships between ihMT signal and intensity of fluorescence microscopy (a quantitative myelin specific histology technique) collected in plp-GFP mouse brain

Inhomogeneous Magnetization Transfer (ihMT) imaging in the acute cuprizone mouse model of demyelination/remyelination

International audienceThis is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain

Epidermal growth factor receptor protein detection in head and neck cancer patients: a many-faceted picture.

International audiencePURPOSE: Epidermal growth factor receptor (EGFR) overexpression is associated with poor prognosis in head and neck squamous cell carcinoma (HNSCC). Despite intensive biomarker studies, a consensual method for assessing EGFR protein expression is still lacking. Here we set out to compare three EGFR detection methods in tumor specimens from HNSCC patients. EXPERIMENTAL DESIGN: Tumors were prospectively excised from a series of 79 high-risk HNSCC patients enrolled in a GORTEC-sponsored clinical trial. EGFR expression was determined using a ligand-binding assay on membranes, Western blotting (WB) on membranes and total homogenates, and immunohistochemistry (IHC) on tissue microarrays. In addition, phosphorylated EGFR (pEGFR) was measured by WB on membranes. RESULTS: Distributions and ranges of tumor EGFR expression were method dependent. Moderate positive correlations (Spearman coefficient r ≈ 0.50) were observed between EGFR expression measured by the binding assay and WB or IHC. pEGFR levels positively and significantly correlated with total EGFR expression measured by WB or ligand binding, but not by IHC. The highest correlation (r = 0.85) was observed between EGFR and pEGFR levels, both measured by WB on membranes. Interestingly, the fraction of phosphorylated receptor (pEGFR/EGFR both measured by WB on membranes) significantly declined with increasing tumor EGFR expression, by all assessment methods used. CONCLUSION: This study shows significant correlations between EGFR detection methods. The observed relationships between EGFR and pEGFR indicate that high-throughput pEGFR/EGFR analyses merit further investigations and consideration for routine use in patient samples