8 research outputs found
Genetic Homogeneity of the Invasive Lionfish Across the Northwestern Atlantic and the Gulf of Mexico Based On Single Nucleotide Polymorphisms
Despite the devastating impact of the lionfish (Pterois volitans) invasion on NW Atlantic ecosystems, little genetic information about the invasion process is available. We applied Genotyping by Sequencing techniques to identify 1,220 single nucleotide polymorphic sites (SNPs) from 162 lionfish samples collected between 2013 and 2015 from two areas chronologically identified as the first and last invaded areas in US waters: the east coast of Florida and the Gulf of Mexico. We used population genomic analyses, including phylogenetic reconstruction, Bayesian clustering, genetic distances, Discriminant Analyses of Principal Components, and coalescence simulations for detection of outlier SNPs, to understand genetic trends relevant to the lionfish’s long-term persistence. We found no significant differences in genetic structure or diversity between the two areas (FST p-values \u3e 0.01, and t-test p-values \u3e 0.05). In fact, our genomic analyses showed genetic homogeneity, with enough gene flow between the east coast of Florida and Gulf of Mexico to erase previous signals of genetic divergence detected between these areas, secondary spreading, and bottlenecks in the Gulf of Mexico. These findings suggest rapid genetic changes over space and time during the invasion, resulting in one panmictic population with no signs of divergence between areas due to local adaptation
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Cloning and partial sequence analysis of multiple P450 genes from the marine fish, Fundulus Heteroclitus
Diversity of cytochrome P450 2 family genes in non-mammalian vertebrates
The diversity and multiplicity of CYP2 genes in early diverging vertebrates were assessed using RT-PCR to identify sequences in an elasmobranch (dogfish:
Squalus acanthias), a teleost (scup:
Stenotomus chrysops), an amphibian (mudpuppy:
Necturus maculosus), a reptile (turtle:
Chrysemys picta) and a bird (chicken:
Gallus gallus). Sixteen novel sequences were amplified using total RNA from liver of dogfish, turtle, mudpuppy and chicken. Two turtle sequences, four mudpuppy sequences and one dogfish sequence clustered with frog CYP2Q and chicken CYP2H genes. One turtle sequence grouped with CYP2E. A new chicken CYP2 clustered with rat CYP2B, CYP2G and CYP2A and trout CYP2M. A dogfish sequence and a turtle sequence grouped with CYP2D and CYP2K. Eight additional sequences were amplified from scup liver, intestine, brain and heart; these all grouped in a CYP2J/2N/2P clade. One sequence,
prov. scup CYP2N3 (but possibly a CYP2N1 orthologue), was isolated from scup heart, gut, liver and brain. In the brain, cDNAs were amplified from the olfactory lobe, medulla, infundibulum and pituitary. One sequence was most highly expressed in the pituitary, where it may function in some aspect of hormonal control. Distinct CYP2P-related sequences were isolated from scup liver (
prov. CYP2P4) and heart (
prov. CYP2P5). Addition-al sequences from scup heart and brain RNA also grouped within this clade but could not be assigned to a subfamily. These results greatly expand the number of non-mammalian CYP2 genes known. Non-mammalian sequences may help to define CYP2 subfamily relationships and to identify conserved groups and functions. [Support: Sea Grant NA46RG0470, R/P60.