20 research outputs found
Application of Pulsed Field Gel Electrophoresis to Determine Îł-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules
Get PDFThe frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to Îł-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of the gels were employed: (1) The DNA mass distribution profile obtained by electrophoresis was compared to computed profiles, and the number of DSB per unit length was then derived in terms of a fitting procedure; (2) hybridization of selected chromosomes was performed, and a comparison of the hybridization signals in treated and untreated samples was then used to derive the frequency of dsb
APOPTOSIS DURING PHOTODYNAMIC THERAPY-INDUCED ABLATION OF RIF-1 TUMORS IN C3H MICE: ELECTRON MICROSCOPIC, HISTOPATHOLOGIC AND BIOCHEMICAL EVIDENCE
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Influence of chronic exposure to low doses of Îł-radiation and 90Sr on the level of DNA breaks and cell sensitivity to hydrogen peroxide in the mouse spleen
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Resistance against cycloheximide in cell lines from Chinese hamster and human cells is conferred by the large subunit of cytoplasmic ribosomes
No full textThe repair of double-strand breaks and S1 nuclease-sensitive sites can be monitored chromosome-specifically in Saccharomyces cerevisiae using pulsed-field gel electrophoresis
No full textErythromycin resistant mutations inBacillus subtilis cause temperature sensitive sporulation
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