In-vitro antioxidant activities of the stem bark extract fractions of Bridelia ferruginea

Bridelia ferruginea stem bark generally used in Indigenous folk medicine for diverse uses was evaluated scientifically to elucidate the antioxidant activity of various fractions in-vitro to validate its folkloric usage. In vitro antioxidant properties of the extract fractions were evaluated using the free radical scavenging activities by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) with ascorbic acid as control. The total antioxidant activity results indicated that, the inhibition percent of aqueous extract was significantly higher than the inhibition percent of ethanol and ethyl acetate in the DPPH methods. A higher IC50 (0.85 mg/ml compared to 1.34 mg/ml ethanolic extract and 1.41mg/ml of Ethyl acetate) value was observed. The aqueous extract was better in Fe2+chelating activity and higher in the content of total phenol as compared to ethanolic and ethyl acetate extracts. However, the ethyl acetate extract had signi?cantly higher (P < 0.05) hydroxyl radical scavenging activity with the concentration for 50% inhibition (IC) value of 0.86 mg/mL, 1.24 mg/mL for ethanolic extract and 2.16 mg/mL for aqueous extract. The results indicated that all the extracts showed excellent Nitric oxide scavenging activities with a close range of IC50 values of 0.83, 0.99 and 1.83 mg/mL for aqueous, ethanolic and ethyl acetate extract. The results suggest that Bridelia ferruginea stem bark possesses varied degrees of antioxidant activity in vitro and has the potential to be developed into dietary supplements and synergically modified with synthetic antioxidants. Keywords: Ethanolic extract, Aqueous extract, Ethyl acetate extract, Bridelia ferruginea, and Antioxidant activity

Antioxidant and Inhibitory Effect of Scent Leaf (Ocimum gratissimum) on Fe2+ and Sodium Nitroprusside Induced Lipid Peroxidation in Rat Brain In vitro

Neurodegenerative diseases have been linked to oxidative stress arising from peroxidation of membrane biomolecules and high levels of Fe and Sodium nitroprusside have been reported to play an important role in neurodegenerative diseases and other brain disorder. Therefore, this study sought to investigate the inhibitory effect of aqueous, ethanolic and ethyl acetate extract of Ocimum gratissimum leaves on FeSO4 and Sodium Nitroprusside (SNP) induced lipid peroxidation in rat brain in vitro. Incubation of the brain tissue homogenate in the presence of FeSO4 and SNP showed both pro-oxidants [Fe and sodium 2+ nitroprusside (SNP)] caused a significantly reduction in (p<0.05) the accumulation of lipid peroxides in a concentration dependent manner. However, the ethyl acetate fraction significantly (P < 0.05) inhibited Fe2+ induced oxidative stress in the rat brain tissue homogenates than the aqueous and ethanolic extract respectively. This higher inhibitory effect of Ocimum gratissimum could be attributed to its significantly higher phytochemical content, Fe2+ chelating ability, hydroxyl scavenging ability, total phenolic content and reducing power. However, part of the mechanisms through which the extractable phytochemicals in Ocimum gratissimum protect the brain may be through their antioxidant activity, Fe2+ chelating, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and OH scavenging ability. Therefore, oxidative stress in the brain could be potentially managed/prevented by dietary intake of Ocimum gratissimum

Protective effect of Irvingia gabonensis stem bark extract on cadmiuminduced nephrotoxicity in rats.

Cadmium has been considered a risk factor for humans as it accumulates in body tissues, such as the liver, lungs, kidneys, bones, and reproductive organs. The aim of the present study was to evaluate the effect of Irvingia gabonensis (IG) against cadmium (Cd)-induced nephrotoxicity. The study was performed on twenty (20) male rats divided into four groups: control group, cadmium group (4 mg/kg/day, intraperitoneally), cadmium + extract (200 mg/kg body weight by oral gavage) and cadmium + extract (400 mg/kg body weight by oral gavage). Changes in the kidney biochemical markers, namely glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), aminotransferase (ALT), aspartate aminotransferase (AST) activities and levels of malondialdehyde (MDA), urea, and creatinine were determined in serum. Histological examinations were monitored. Exposure to Cd lowered the activities of kidney antioxidants, while it increased LPO levels. Levels of all disrupted parameters were alleviated by co-administration of IG extract. The malondialdehyde concentration of the rats treated with 200 and 400 mg/kg body weight of the extract significantly decreased (p<0.05) compared with the untreated cadmium rats. Yet the creatinine concentration decreased significantly (p<0.05) when the cadmium animals treated with 200 and 400 mg/kg body weight of the extract were compared with the cadmium control. Furthermore, histological alterations in the kidney were observed in cadmium untreated rats and these were ameliorated in cadmium treated rats by co-administration of IG extract. IG showed apparent protective and curative effect on Cd-induced nephrotoxicity

Protective effect of Irvingia gabonensis stem bark extract on cadmiuminduced nephrotoxicity in rats

Due to a malfunction inside Platt's camera, the majority of his first 8,000 images, dating from 1919 to 1933, have blurred areas where the unexposed film was not laying flat inside the camera. His 35mm photography after 1933 does not show this defect, but the condition of the film is rather poor, having been housed between loose sheets of paper in cardboard boxes for over 50 years. The majority of images he took from 1933-1963 are severely scratched. Therefore, only a small portion of Platt's photography has been chosen for online presentation.8/13 Mill & flume fr.[from] Lake shore.Grayscal

Protective effect of Irvingia gabonensisstem bark extract on cadmium-induced nephrotoxicity in rats

Cadmium has been considered a risk factor for humans as it accumulates in body tissues, such as the liver, lungs, kidneys, bones, and reproductive organs. The aim of the present study was to evaluate the effect of Irvingia gabonensis (IG) against cadmium (Cd)-induced nephrotoxicity. The study was performed on twenty (20) male rats divided into four groups: control group, cadmium group(4 mg/kg/day, intraperitoneally), cadmium + extract (200 mg/kg body weight by oral gavage) and cadmium + extract (400 mg/kg body weight by oral gavage). Changes in the kidney biochemical markers, namely glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), aminotransferase (ALT), aspartate aminotransferase (AST) activities and levels of malondialdehyde (MDA), urea, and creatinine were determined in serum. Histological examinations were monitored. Exposure to Cd lowered the activities of kidney antioxidants, while it increased LPO levels. Levels of all disrupted parameters were alleviated by co-administration of IG extract. The malondialdehyde concentration of the rats treated with 200 and 400 mg/kg body weight of the extract significantly decreased (p<0.05) compared with the untreated cadmium rats. Yet the creatinine concentration decreased significantly (p<0.05) when the cad-mium animals treated with 200 and 400 mg/kg body weight of the extract were compared with the cadmium control. Furthermore, histological alterations in the kidney were observed in cadmium untreated rats and these were ameliorated in cadmium treated rats by co-administration of IG extract. IG showed apparent protective and curative effect on Cd-induced nephrotoxicit