Ternary complex formation between DNA-adenovirus core protein VII and TAF-Iβ/SET, an acidic molecular chaperone

AbstractThe adenovirus (Ad) genome complexed with viral core proteins designated Ad core is the template for transcription of early genes and the first round of replication in Ad-infected cells. A cellular protein designated template-activating factor-I (TAF-I) is found to be involved in remodeling of the Ad core in vitro. Here we found that TAF-I interacts with the Ad DNA through core protein VII in infected cells in early phases of infection. In vitro binding assays using recombinant proteins showed that TAF-I forms ternary complexes with DNA–protein VII complexes

A Multiplex Rupture Sequence Under Complex Fault Network Due To Preceding Earthquake Swarms During the 2024 Mw 7.5 Noto Peninsula, Japan, Earthquake

「令和6年能登半島地震」は長く静かに始まり、向きや傾斜の異なる断層を次々と破壊した.京都大学プレスリリース. 2024-06-13.A devastating earthquake with moment magnitude 7.5 occurred in the Noto Peninsula in central Japan on 1 January 2024. We estimate the rupture evolution of this earthquake from teleseismic P-wave data using the potency-density tensor inversion method, which provides information on the spatiotemporal slip distribution including fault orientations. The results show a long and quiet initial rupture phase that overlaps with regions of preceding earthquake swarms and associated aseismic deformation. The following three major rupture episodes evolve on segmented, differently oriented faults bounded by the initial rupture region. The irregular initial rupture process followed by the multi-scale rupture growth is considered to be controlled by the preceding seismic and aseismic processes and the geometric complexity of the fault system. Such a discrete rupture scenario, including the triggering of an isolated fault rupture, adds critical inputs on the assessment of strong ground motion and associated damages for future earthquakes

Multi‐Scale Rupture Growth With Alternating Directions in a Complex Fault Network During the 2023 South‐Eastern Türkiye and Syria Earthquake Doublet

A devastating doublet of earthquakes with moment magnitude MW 7.9 and MW 7.6 earthquakes contiguously occurred in SE Türkiye near the NW border of Syria. Here we perform a potency-density tensor inversion to simultaneously estimate rupture evolution and fault geometry for the doublet. We find the initial MW 7.9 earthquake involved discrete episodes of supershear rupture and back-rupture propagation, and was triggered by initial rupture along a bifurcated splay of the East Anatolian Fault. The second MW 7.6 event was triggered by the earlier MW 7.9 event, and it involved more extensive supershear rupture along a favorably curved fault, and was likely stopped by geometric barriers at the fault ends. Our results highlight the multi-scale cascading rupture growth across the complex fault network that affects the diverse rupture geometries of the 2023 Türkiye earthquake doublet, contributing to the strong ground shaking and associated devastation

Late-onset benefit in progressive advanced hepatocellular carcinoma with continued sorafenib therapy: a case report

<p>Abstract</p> <p>Introduction</p> <p>In the past, no effective systemic therapy has existed for patients with advanced hepatocellular carcinoma. Sorafenib, an oral multikinase inhibitor, has recently been shown to improve overall survival in patients with advanced hepatocellular carcinoma in two randomized, double-blinded, placebo-controlled trials. This drug has been approved as the first-line therapy for advanced hepatocellular carcinoma patients. We report an intriguing case of advanced hepatocellular carcinoma in which the patient achieved late- onset partial response by prolonged administration of sorafenib in spite of progressive disease.</p> <p>Case presentation</p> <p>A 54-year-old Japanese man was treated with sorafenib for multiple lung metastases after surgical resection for advanced hepatocellular carcinoma accompanied by vascular invasion of the left branch of the portal vein. Although the effective diagnosis was progressive disease, almost all sites began to reduce or disappear eight months after the diagnosis of progressive disease. A dramatic reduction in alpha-fetoprotein and des-gamma-carboxy prothrombin levels was observed. The patient finally achieved partial response and his status remains unchanged.</p> <p>Conclusions</p> <p>If tolerated, prolonged sorafenib treatment may be beneficial.</p

Consecutive ruptures on a complex conjugate fault system during the 2018 Gulf of Alaska earthquake

2018年アラスカ湾地震の複雑な破壊過程を解析 --間欠的に加速・減速する奇妙な巨大地震--. 京都大学プレスリリース. 2021-03-22.We developed a flexible finite-fault inversion method for teleseismic P waveforms to obtain a detailed rupture process of a complex multiple-fault earthquake. We estimate the distribution of potency-rate density tensors on an assumed model plane to clarify rupture evolution processes, including variations of fault geometry. We applied our method to the 23 January 2018 Gulf of Alaska earthquake by representing slip on a projected horizontal model plane at a depth of 33.6 km to fit the distribution of aftershocks occurring within one week of the mainshock. The obtained source model, which successfully explained the complex teleseismic P waveforms, shows that the 2018 earthquake ruptured a conjugate system of N-S and E-W faults. The spatiotemporal rupture evolution indicates irregular rupture behavior involving a multiple-shock sequence, which is likely associated with discontinuities in the fault geometry that originated from E-W sea-floor fracture zones and N-S plate-bending faults

Development and application of a tsunami fragility curve of the 2015 tsunami in Coquimbo, Chile

The last earthquake that affected the city of Coquimbo took place in September 2015 and had a magnitude of Mw = 8.3, resulting in localized damage in low-lying areas of the city. In addition, another seismic gap north of the 2015 earthquake rupture area has been identified; therefore, a significant earthquake (Mw = 8.2 to 8.5) and tsunami could occur in the near future. The present paper develops a tsunami fragility curve for the city of Coquimbo based on field survey data and tsunami numerical simulations. The inundation depth of the 2015 Chile tsunami in Coquimbo was estimated by means of numerical simulation with the Non-hydrostatic Evolution of Ocean WAVEs (NEOWAVE) model and five nested grids with a maximum grid resolution of 10m. The fragility curve exhibited behavior similar to that of other curves in flat areas in Japan, where little damage was observed at relatively high inundation depths. In addition, it was observed that Coquimbo experienced less damage than Dichato (Chile); in fact, at an inundation depth of 2m, Dichato had a  ∼ 75% probability of damage, while Coquimbo proved to have only a 20% probability. The new fragility curve was used to estimate the damage by possible future tsunamis in the area. The damage assessment showed that  ∼ 50% of the structures in the low-lying area of Coquimbo have a high probability of damage in the case of a tsunami generated off the coast of the study area if the city is rebuilt with the same types of structures

高周波励起源と断層すべりに基づく巨大地震の不規則な発展プロセスの解明

この博士論文は全文公表に適さないやむを得ない事由があり要約のみを公表していましたが、解消したため、令和3(2021)年1月25日に全文を公表しました。筑波大学 (University of Tsukuba)201

高周波励起源と断層すべりに基づく巨大地震の不規則な発展プロセスの解明

筑波大学 (University of Tsukuba)201

Application of TensorFlow to recognition of visualized results of fragment molecular orbital (FMO) calculations

We have applied Google's TensorFlow deep learning toolkit to recognize the visualized results of the fragment molecular orbital (FMO) calculations. Typical protein structures of alpha-helix and beta-sheet provide some characteristic patterns in the two-dimensional map of inter-fragment interaction energy termed as IFIE-map (Kurisaki et al., Biophys. Chem. 130 (2007) 1). A thousand of IFIE-map images with labels depending on the existences of alpha-helix and beta-sheet were prepared by employing 18 proteins and 3 non-protein systems and were subjected to training by TensorFlow. Finally, TensorFlow was fed with new data to test its ability to recognize the structural patterns. We found that the characteristic structures in test IFIE-map images were judged successfully. Thus the ability of pattern recognition of IFIE-map by TensorFlow was proven.Comment: 26 pages, 3 figures, 4 table