Elevated serum levels of CXCL9/ monokine induced by interferon-gamma and CXCL10/interferongamma-inducible protein-10 in ocular sarcoidosis. Invest Ophthalmol Vis Sci

PURPOSE. Sarcoidosis is a chronic multisystem granulomatous disorder characterized by an accumulation of activated CD4 ϩ T cells and monocytes/macrophages in involved organs. Chemokines are required for the extravasation of leukocytes to the inflammation site. This study was undertaken to determine which chemokines are augmented in the serum of patients with active ocular sarcoidosis. METHODS. Seventeen patients with diagnosed ocular sarcoidosis, 28 with suspected ocular sarcoidosis, 16 with Behçet's disease, 17 with Vogt-Koyanagi-Harada disease, and 18 healthy subjects were studied. Serum levels of CCL2, CCL5, CXCL8, CXCL9, and CXCL10 were simultaneously measured by cytometric bead array using flow cytometer. In addition, serum CXCL9 and CXCL10 levels in the patients with diagnosed or suspected ocular sarcoidosis were compared with respect to ocular disease activity, the presence of bilateral hilar lymphadenopathy (BHL), and laboratory data. RESULTS. Serum levels of both CXCL9 and CXCL10 were markedly elevated in the patients with diagnosed or suspected ocular sarcoidosis compared with patients with other types of uveitis and healthy subjects. Although CCL2 and CXCL8 were detected in the serum of all subjects, the levels were extremely low with no significant differences between groups. Elevation of serum CXCL9 and CXCL10 in ocular sarcoidosis correlated significantly with ocular disease activity and ACE (angiotensin converting enzyme) levels and was unrelated to the presence of BHL, erythrocyte sedimentation rate, white blood cell count, serum IgG, or serum lysozyme. CONCLUSIONS. The results demonstrated that serum levels of CXCL9 and CXCL10 were elevated markedly in the patients with ocular sarcoidosis and correlated with ocular disease activity and ACE level. (Invest Ophthalmol Vis Sci

Detection of autoantigens in experimental autoimmune uveoretinitis (EAU)

Shown is a two-dimensional gel image of murine retinal proteins stained with SYPRO Ruby. Approximately 2,000 spots were observed. Among the SYPRO Ruby stained protein spots, the 2D-WB positive spots either in control or EAU were randomly numbered. The numbers and the positions of the seven candidate autoantigens in EAU on a SYPRO Ruby stained gel are shown in panel . The spot numbers of the candidate autoantigens are common between panel , , and . Extracted murine retinal proteins were separated by two-dimensional electrophoresis, transferred onto nitrocellulose membranes. Western blotting was performed using sera from EAU or control mice. Representative membranes reacted with sera from complete Freund's adjuvant-treated control mice are shown in subpanel , and those reacted with sera from EAU mice are shown in subpanel . Each set of and subpanels shows the corresponding area. Arrowheads indicate the position of each candidate autoantigen on the EAU membranes. membranes.<p><b>Copyright information:</b></p><p>Taken from "Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens"</p><p></p><p>Molecular Vision 2008;14():1094-1104.</p><p>Published online 12 Jun 2008</p><p>PMCID:PMC2426731.</p><p></p

Autoantibodies against the retinal autoantigens detected in experimental autoimmune uveoretinitis were tested using sera from human endogenous uveitis patients

The antibody titer was calculated as binding units according to the formula given in Methods. Statistically significant difference of positive rate between each patient group and healthy control (HC) was detected in Behcet’s disease (BD; p=0.016) and Vogt-Koyanagi-Harada disease (VKH; p=0.015) for anti-EsteD antibody, and in VKH (p=0.015) and sarcoidosis (p=0.036) for anti- brain-type creatine kinase (BB-CK).<p><b>Copyright information:</b></p><p>Taken from "Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens"</p><p></p><p>Molecular Vision 2008;14():1094-1104.</p><p>Published online 12 Jun 2008</p><p>PMCID:PMC2426731.</p><p></p