Cervical Cancer Screening by Female Workers in South East Nigeria

Cervical cancer is one of the commonest female cancers especially in developing countries. Efforts towards its prevention worldwide have focused on screening women at risk of disease using Pap smears and treating pre-cancerous lesions. A good knowledge and understanding of the level of practice of cervical cancer screening among female workers in south east Nigeria will help in creating population-specific healthcare programs and interventions aimed at improving women’s health. OBJECTIVES: To determine the perception and practice of cervical cancer screening among female workers in south east Nigeria as well as ascertain the influence of age, educational status and marital status on the practice of cervical cancer screening among this group. METHOD: A cross-sectional descriptive study carried out among female workers in Nnewi who were selected using multi-stage approach between December 2007 and January 2008. Tests of statistical significance were done using chi square test at 95% confidence interval. RESULTS A total of 172 females responded. The mean age was 29.7 ± 8.8 years and the ages ranged from 15 to 65 years. One hundred and twenty-three respondents (71.5%) knew about cervical cancer screening. Only 12 (9.8%) of the respondents who were aware of Pap smear had done the test, of which 9 (75.0%) had disease detected. There was no statistically significant association between educational level, age andmarital status respectively and the practice of cervical cancer screening. CONCLUSION: There is avery lowlevel of practice of cervical cancer screening in this group. Educational level, age and marital status were found not to affect the practice of cervical cancer screening. Key Words: Cervical cancer, workers, practice, Nigeri

Illustrated State-of-the-Art Capsules of the ISTH 2023 Congress

This year’s Congress of the International Society of Thrombosis and Haemostasis (ISTH) took place in person in Montréal, Canada, from June 24-28, 2023. The conference, held annually, highlighted cutting-edge advances in basic, translational, population and clinical sciences relevant to the Society. As for all ISTH congresses, we offered a special, congress-specific scientific theme; this year, the special theme was immunothrombosis. Certainly, over the last few years, COVID-19 infection and its related thrombotic and other complications have renewed interest in the concepts of thromboinflammation and immunothrombosis; namely, the relationship between inflammation, infection and clotting. Other main scientific themes of the Congress included Arterial Thromboembolism, Coagulation and Natural Anticoagulants, Diagnostics and Omics, Fibrinolysis and Proteolysis, Hemophilia and Rare Bleeding Disorders, Hemostatic System in Cancer, Inflammation and Immunity, Pediatrics, Platelet Disorders, von Willebrand Disease and Thrombotic Microangiopathies, Platelets and Megakaryocytes, Vascular Biology, Venous Thromboembolism and Women’s Health. Among other sessions, the program included 28 State-of-the-Art (SOA) sessions with a total of 84 talks given by internationally recognized leaders in the field. SOA speakers were invited to prepare brief illustrated reviews of their talks that were peer reviewed and are included in this article. These illustrated capsules highlight the major scientific advances with potential to impact clinical practice. Readers are invited to take advantage of the excellent educational resource provided by these illustrated capsules. They are also encouraged to use the image in social media to draw attention to the high quality and impact of the science presented at the Congress

Circulating Plasma microRNAs can differentiate Human Sepsis and Systemic Inflammatory Response Syndrome (SIRS)

Systemic inflammation in humans may be triggered by infection, termed sepsis, or non-infective processes, termed non-infective systemic inflammatory response syndrome (SIRS). MicroRNAs regulate cellular processes including inflammation and may be detected in blood. We aimed to establish definitive proof-of-principle that circulating microRNAs are differentially affected during sepsis and non-infective SIRS. Critically ill patients with severe (n = 21) or non-severe (n = 8) intra-abdominal sepsis; severe (n = 23) or non-severe (n = 21) non-infective SIRS; or no SIRS (n = 16) were studied. Next-generation sequencing and qRT-PCR were used to measure plasma microRNAs. Detectable blood miRNAs (n = 116) were generally up-regulated in SIRS compared to no-SIRS patients. Levels of these 'circulating inflammation-related microRNAs' (CIR-miRNAs) were 2.64 (IQR: 2.10-3.29) and 1.52 (IQR: 1.15-1.92) fold higher for non-infective SIRS and sepsis respectively (p < 0.0001), hence CIR-miRNAs appeared less abundant in sepsis than in SIRS. Six CIR-miRNAs (miR-30d-5p, miR-30a-5p, miR-192-5p, miR-26a-5p, miR-23a-5p, miR-191-5p) provided good-to-excellent discrimination of severe sepsis from severe SIRS (0.742-0.917 AUC of ROC curves). CIR-miRNA levels inversely correlated with pro-inflammatory cytokines (IL-1, IL-6 and others). Thus, among critically ill patients, sepsis and non-infective SIRS are associated with substantial, differential changes in CIR-miRNAs. CIR-miRNAs may be regulators of inflammation and warrant thorough evaluation as diagnostic and therapeutic targets

IFNγ and IL-12 restrict Th2 responses during Helminth/Plasmodium co-infection and promote IFNγ from Th2 cells

Parasitic helminths establish chronic infections in mammalian hosts. Helminth/Plasmodium co-infections occur frequently in endemic areas. However, it is unclear whether Plasmodium infections compromise anti-helminth immunity, contributing to the chronicity of infection. Immunity to Plasmodium or helminths requires divergent CD4+ T cell-driven responses, dominated by IFNγ or IL-4, respectively. Recent literature has indicated that Th cells, including Th2 cells, have phenotypic plasticity with the ability to produce non-lineage associated cytokines. Whether such plasticity occurs during co-infection is unclear. In this study, we observed reduced anti-helminth Th2 cell responses and compromised anti-helminth immunity during Heligmosomoides polygyrus and Plasmodium chabaudi co-infection. Using newly established triple cytokine reporter mice (Il4gfpIfngyfpIl17aFP635), we demonstrated that Il4gfp+ Th2 cells purified from in vitro cultures or isolated ex vivo from helminth-infected mice up-regulated IFNγ following adoptive transfer into Rag1-/- mice infected with P. chabaudi. Functionally, Th2 cells that up-regulated IFNγ were transcriptionally re-wired and protected recipient mice from high parasitemia. Mechanistically, TCR stimulation and responsiveness to IL-12 and IFNγ, but not type I IFN, was required for optimal IFNγ production by Th2 cells. Finally, blockade of IL-12 and IFNγ during co-infection partially preserved anti-helminth Th2 responses. In summary, this study demonstrates that Th2 cells retain substantial plasticity with the ability to produce IFNγ during Plasmodium infection. Consequently, co-infection with Plasmodium spp. may contribute to the chronicity of helminth infection by reducing anti-helminth Th2 cells and converting them into IFNγ-secreting cells

Perception and Practice of Periodic Medical Checkup by Traders in South East Nigeria

BACKGROUND Periodic medical checkup helps improve life expectancy. Traders are a major economically active population in south eastern Nigeria and are expected to benefit from periodic medical checkup. A good knowledge and understanding of the prevailing health knowledge, attitude and preventive health behaviour of these traders is essential for creating population-specific health programs. OBJECTIVES To determine the knowledge, attitude and practice of periodic medical checkup by traders in South east Nigeria, as well as the influence of age, gender and educational status on its practice in this group. METHODS: A questionnaire- based descriptive study carried out among traders in Nnewi, South east Nigeria, who were selected randomly between September and October 2010. A total of 323 traders responded, 252 males (78.0%) and 71(22.0%)females. Themeanagewas 31.9± 10 years and the ages ranged from15 to 65 years withmajority(49.2%) between 20-29 years. Majority (61.0%) had secondary school education and 1.2% had post secondary school education. About 74.9% were aware of periodic medical checkup; all females and 67.9% of males. Major source of information was through friends (61.2%), then mass media(18.2%). The commonest known type of medical checkup was general examination (60.7%), then blood pressure measurement (55.4%). About 63.8% feel everybody needs medical checkup. Most (85.5%) feel medical checkups can improve their work efficiency. Only 29.4% of males and 39.4% of females practice periodic medical checkup. Age, gender and educational status were found not to affect practice of periodic medical checkup significantly. CONCLUSION: This study revealed a high level of awareness of periodic medical checkup, but a very low level of practice among this group. Effort should be made by health agencies to educate traders on the various types of medical check up, their indications and benefits as well as its practice encouraged. Keywords: Periodic medical checkup, traders,Nigeria

Health risks for a rural community in Bokkos, Plateau State, Nigeria, exposed to potentially toxic elements from an abandoned tin mine

The past mining activities in Bokkos Local Government Area (LGA) were performed in an uncontrolled way and gave rise to many abandoned ponds now serving as domestic and irrigation water sources. Past research focused mainly on the environmental impact, and we show for the first time what the human health risk through consumption of contaminated food crops is in these communities. This study was designed to determine the level of Potentially Toxic Elements (PTEs) contamination in pond water, soil, and food crops and assess the health risk of inhabitants in the abandoned tin mining community in Bokkos LGA. Samples of the mining pond water, soil, and selected food crops from farms irrigated with the pond water: bitter leaf (Vernonia amygdalina), pepper (Piper nigrum), okra (Albelmoschus esculentus), maize (Zea mays), sweet potato (Ipomoea batatas), and Irish potato (Solanum tuberosum) were analyzed for each of the eight PTEs (viz. Cu, Cr, Fe, Mn, Ni, Zn, Cd, and Pb) using inductively coupled plasma optical emission spectrometry (ICP-OES). The results obtained showed that the levels of all the PTEs analyzed in the soil, pond water, and selected food crops except for Fe and Mn in soil and Cd in sweet potato were greater than their corresponding background area values (p -3-10-1 which is several fold higher than the permissible limits (10-6 and -4) indicating the high carcinogenic risk. It can be concluded based on the results and risk assessment provided by this study that human exposure to mining pond water and soil in farms around the mining pond through the food chain suggests the high vulnerability of the local community to PTE toxicity. Long-term preventive measures to safeguard the health of the residents need to be put in place

Women at altitude: carbohydrate utilization during exercise at 4,300 m

To evaluate the hypothesis that exposure to high altitude would reduce blood glucose and total carbohydrate utilization relative to sea level (SL), 16 young women were studied over four 12-day periods: at 50% of peak O2 consumption in different menstrual cycle phases (SL-50), at 65% of peak O2 consumption at SL (SL-65), and at 4,300 m (HA). After 10 days in each condition, blood glucose rate of disappearance (Rd) and respiratory exchange ratio were measured at rest and during 45 min of exercise. Glucose Rd during exercise at HA (4.71 ± 0.30 mg ⋅ kg−1 ⋅ min−1) was not different from SL exercise at the same absolute intensity (SL-50 = 5.03 mg ⋅ kg−1 ⋅ min−1) but was lower at the same relative intensity (SL-65 = 6.22 mg ⋅ kg−1 ⋅ min−1, P &lt; 0.01). There were no differences, however, when glucose Rd was corrected for energy expended (kcal/min) during exercise. Respiratory exchange ratios followed the same pattern, except carbohydrate oxidation remained lower (−23.2%, P &lt; 0.01) at HA than at SL when corrected for energy expended. In women, unlike in men, carbohydrate utilization decreased at HA. Relative abundance of estrogen and progesterone in women may partially explain the sex differences in fuel utilization at HA, but subtle differences between menstrual cycle phases at SL had no physiologically relevant effects. </jats:p