Immunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice

Cataloged from PDF version of article.Pasteurella multocida serotype A:3 is a Gram-negative bacterial pathogen, one of the causative agents of shipping fever of cattle. In this study, outer membrane protein H (ompH) and Pasteurella lipoprotein E (plpE) genes were cloned and plpEC-ompH fusion was constructed and expressed in Escherichia coli. Recombinant PlpE, OmpH and PlpEC-OmpH fusion proteins were purified and formulated with oil-based and oil-based CpG ODN adjuvants. Antibody responses in mice vaccinated with recombinant PlpE and PlpEC-OmpH proteins formulated with both adjuvants were significantly (p<0.05) increased. However, a significant (p<0.05) increment in serum IFN-γ level was only observed upon immunization with oil-based CpG formulations. Protectivity of the vaccines were evaluated via intraperitoneal challenge of mice with 10 LD50 of P. multocida A:3. The recombinant proteins PlpE and PlpEC-OmpH fusion conferred 100% protection when formulated with oil-based CpG ODN while the protectivity was found to be 80% and 60%, respectively when only oil-based adjuvant was used in respective formulations. These findings indicated that the recombinant PlpE or PlpEC-OmpH fusion proteins formulated with oil-based CpG ODN adjuvant are possible acellular vaccine candidates against shipping fever. © 2012 Elsevier Ltd

Modelling the induced polarization of bentonite-sand mixtures

International audienceSpectral induced polarization (SIP) has become an increasingly popular geophysical method for hydrogeological and environmental applications. These applications include for instance the non-intrusive characterization of the textural and interfacial physicochemical properties of bentonites used as permeability barriers in landfills or to store various types of contaminants including radioactive wastes. Bentonites are mainly constituted of smectites, which have very high specific surface areas (SSA) and cation exchange capacities (CEC). Therefore, these minerals have very high electromigration and polarization current densities responsible for very high in phase and quadrature conductivities, respectively. In addition, in diluted water, the diffuse layer of smectites occupies a large fraction of the pore space and may be therefore considered as part of the pore space. In our approach [1], complex electrical conductivities of saturated unconsolidated bentonite and bentonite-sand mixtures are modeled at different salinities (NaCl) of the bulk pore water using a Donnan equilibrium model coupled to the revisited SIP model of Leroy and Revil [2]. Our complex surface conductivity model considers the DC contribution of the diffuse and Stern layers as well as the electrochemical polarization of the Stern layer coating the grains with different sizes. The macroscopic SIP model is based on the differential effective medium theory and considers the complex surface conductivity of the sand and smectite grains and the complex conductivity of the pore space. In our model, the diffuse layer of quartz sands occupies a small fraction of the pore space and is considered therefore as part of the surface of the grains. Our SIP model predicts very well the low frequency (0.1 Hz - kHz) complex electrical conductivities of bentonite and bentonite-sand mixtures, except for very low frequencies (< 0.1 Hz) where membrane polarization may occur (Figure 1). The in phase conductivity of the sample with a high clay content (20 % in volume) increases slowly with salinity because of the very high DC surface conductivity of smectite. The observed large increase of the in phase and quadrature conductivity of the samples with the clay content (1, 20 and 100% in volume) is also predicted by our model. The quadrature conductivity of the samples with a high clay content is fairly independent on the pore fluid salinity because it is strongly connected with the SSA, CEC and Stern layer of smectite (Figure 1). The in phase conductivity of the sample with a low clay content (1% in volume) increases quickly with the salinity because of its low DC surface conductivity. Its quadrature conductivity also increases quickly with salinity because of the formation of the Stern layer at the surface of quartz sand. Nevertheless, our SIP model can't predict the quadrature conductivity spectra observed at very low frequencies (< 10-1 Hz). The missing polarization mechanism may correspond to membrane polarization and there is an effort to be done to incorporate this contribution in a unified model

Induced polarization of clay-sand mixtures: experiments and modelling

Frequency-domain induced polarization (IP) measurements consist of imposing an alternative sinusoidal electrical current (AC) at a given frequency and measuring the resulting electrical potential difference between two other non-polarizing electrodes. The magnitude of the conductivity and the phase lag between the current and the difference of potential can be expressed into a complex conductivity with the in-phase representing electromigration and a quadrature conductivity representing the reversible storage of electrical charges (capacitive effect) of the porous material. Induced polarization has become an increasingly popular geophysical method for hydrogeological and environmental applications [1]. These applications include for instance the characterization of clay materials used as permeability barriers in landfills or to contain various types of contaminants including radioactive wastes [2]. The goal of our study is to get a better understanding of the influence of the clay content, clay mineralogy, and pore water salinity upon complex conductivity measurements of saturated clay-sand mixtures in the frequency range ~ 1 mHz-12 kHz. The complex conductivity of saturated unconsolidated sand-clay mixtures was experimentally investigated using two types of clay minerals, kaolinite and smectite in the frequency range 1.4 mHz - 12 kHz. Four different types of sample were used, two containing mainly kaolinite (80% of the mass, the remaining containing 15% of smectite and 5% of illite/muscovite; 95% of kaolinite, 5% of illite/muscovite), and the two others containing mainly Na-smectite or Na-Ca-smectite (95% of the mass; bentonite). The experiments were performed with various clay contents (1, 5, 20, and 100 % in volume of the sand-clay mixture) and salinities (distilled water, 0.1 g/L, 1 g/L, and 10 g/L NaCl solution). In total, 44 saturated clay or clay-sand mixtures were prepared. Induced polarization measurements were performed with a cylindrical four-electrode sample-holder (cylinder made of PVC with 30 cm in length and 19 cm in diameter) associated with a SIP-Fuchs II impedance meter and non-polarizing Cu/CuSO4 electrodes (Figure 1). These electrodes were installed at 10 cm from the base of the sample holder and regularly spaced (each 90 degree). The results illustrate the strong impact of the Cationic Exchange Capacity (CEC) of the clay minerals upon the complex conductivity. The amplitude of the in-phase conductivity of the kaolinite-clay samples is strongly dependent to saturating fluid salinity (Figure 2) for all volumetric clay fractions, whereas the in-phase conductivity of the smectite-clay samples is quite independent on the salinity, except at the low clay content (5% and 1% of clay in volume). This is due to the strong and constant surface conductivity of smectite associated with its very high CEC. The quadrature conductivity increases steadily with the CEC and the clay content. We observe that the dependence on frequency of the quadrature conductivity of sand-kaolinite mixtures is more important than for sand-bentonite mixtures. For both types of clay, the quadrature conductivity seems to be fairly independent on the pore fluid salinity (Figure 2) except at very low clay contents (1% kaolinite-clay in volume). This is due to the constant surface site density of Na counter-ions in the Stern layer of clay materials [3]. At the lowest clay content (1%), the magnitude of the quadrature conductivity increases with the salinity, as expected for silica sands. In this case, the surface site density of Na counter-ions in the Stern layer increases with salinity [4]. The experimental data show good agreement with predicted values given by our Spectral Induced Polarization (SIP) model [4]. This complex conductivity model considers the electrochemical polarization of the Stern layer coating the clay particles and the Maxwell-Wagner polarization. We use the differential effective medium theory to calculate the complex conductivity of the porous medium constituted of the grains and the electrolyte. The SIP model includes also the effect of the grain size distribution upon the complex conductivity spectra. Interfacial parameters are estimated using the TLM of Sverjensky [5] for silica and the Donnan model of Tournassat and Appelo [6] for smectite

The zygomatic implant perforated (ZIP) flap: a new technique for combined surgical reconstruction and rapid fixed dental rehabilitation following low-level maxillectomy.

Abstract This aim of this report is to describe the development and evolution of a new surgical technique for the immediate surgical reconstruction and rapid post-operative prosthodontic rehabilitation with a fixed dental prosthesis following low-level maxillectomy for malignant disease. The technique involves the use of a zygomatic oncology implant perforated micro-vascular soft tissue flap (ZIP flap) for the primary management of maxillary malignancy with surgical closure of the resultant maxillary defect and the installation of osseointegrated support for a zygomatic implant-supported maxillary fixed dental prosthesis. The use of this technique facilitates extremely rapid oral and dental rehabilitation within a few weeks of resective surgery, providing rapid return to function and restoring appearance following low-level maxillary resection, even in cases where radiotherapy is required as an adjuvant treatment post-operatively. The ZIP flap technique has been adopted as a standard procedure in the unit for the management of low-level maxillary malignancy, and this report provides a detailed step-by-step approach to treatment and discusses modifications developed over the treatment of an initial cohort of patients

Paraganglioma of the tongue with SDHB gene mutation in a patient with Graves' disease

The authors want to thank Mr. Jose Eduardo Matos (photog-rapher) for his technical assistance.We report a case of an apparently sporadic paraganglioma of the tongue with a germ-line mutation in a female patient with asymptomatic Graves' disease. The tongue is an unusual primary location. Genetic testing is mandatory in all cases. Thyroid gland dysfunction and autoimmune phenomena could be associated with some paragangliomas.S

Steady late quaternary slip rate on the Cinarcik section of the North Anatolian fault near Istanbul, Turkey

The distribution of plate motion between multiple fault strands and how this distribution may evolve remain poorly understood, despite the key implications for seismic hazards. The North Anatolian Fault in northwest Turkey is a prime example of a multistranded continental transform. Here we present the first constraints on late Quaternary slip rates on its northern branch across the Cinarcik Basin in the eastern Marmara Sea. We use both deep penetration and high‐resolution multichannel seismic reflection data with a stratigraphic age model to show that a depocenter has persisted near the fault bend responsible for that transform basin. Successively older depocenters have been transported westward by fault motion relative to Eurasia, indicating a uniform right‐lateral slip rate of 18.5 mm/yr over the last 500,000 years, compared to overall GPS rates (23–24 mm/yr). Thus, the northern branch has slipped at a nearly constant rate and has accounted for most of the relative plate motion between Eurasia and Anatolia since ~0.5 Ma

Mutations in LAMB1 Cause Cobblestone Brain Malformation without Muscular or Ocular Abnormalities

Cobblestone brain malformation (COB) is a neuronal migration disorder characterized by protrusions of neurons beyond the first cortical layer at the pial surface of the brain. It is usually seen in association with dystroglycanopathy types of congenital muscular dystrophies (CMDs) and ocular abnormalities termed muscle-eye-brain disease. Here we report homozygous deleterious mutations in LAMB1, encoding laminin subunit beta-1, in two families with autosomal-recessive COB. Affected individuals displayed a constellation of brain malformations including cortical gyral and white-matter signal abnormalities, severe cerebellar dysplasia, brainstem hypoplasia, and occipital encephalocele, but they had less apparent ocular or muscular abnormalities than are typically observed in COB. LAMB1 is localized to the pial basement membrane, suggesting that defective connection between radial glial cells and the pial surface mediated by LAMB1 leads to this malformation