Plutonium concentration and 240Pu/239Pu atomic ratio in liver from

財団法人 日本分析センター元・金沢大学大学院自然科学研究科金沢大学環日本海域環境研究センター地球環境計測研究部門金沢大学理学部Plutonium isotopes, 239Pu and 240Pu, were measured in liver samples from Surume squid using a sector-field high resolution ICP-MS after radiochemical purification. Surume squid samples were obtained from nine landing ports in Japanese inshore during fishery season from September to December 2002. Concentrations of 239Pu and 240Pu ranged from 1.5 to 28 mBq kg-1 and 1.1 to 24 mBq kg-1, respectively. Plutonium (239,240Pu) concentrations in liver were several thousand times higher than levels found in seawater. The concentration factor (CF) compared to seawater for 239,240Pu and 13 other elements ranged from 100 to 107. The CF values for 239,240Pu, V and Th were 102-104. Pu had an intermediate CF between conservative and scavenged elements. 240Pu/239Pu atomic ratios in the squid liver ranged from 0.177 to 0.237 which were slightly higher than 0.178 ± 0.014 for global fallout. The variations of 240Pu/239Pu atomic ratios in ocean currents with different source functions are important for interpreting high 240Pu/239Pu atomic ratios in Surume squid liver. It seems likely that Pu with high 240Pu/239Pu atomic ratio is continuously transported through the solubilization and seawater transport from the North Equatorial Current to Kuroshio and its branch, Tsushima Current. By assuming that Pu found in Surume squid liver is a mixture of global fallout Pu (0.178) and close-in fallout Pu with high 240Pu/239Pu atomic ratio (0.30-0.36) around Bikini Atoll, Pu contribution from Bikini close-in fallout Pu accounts for close to 35% of the whole plutonium in Surume squid liver. These results highlight that Surume squid is a useful organism for evaluating environmental Pu levels of larger sea area and facilitate the development of models to understand oceanic transport of close-in fallout Pu from Bikini Atoll. © 2007 Elsevier Ltd. All rights reserve

Development of low level plutonium measurement by ICP mass spectrometry and its application to marine environment: Pu isotopes and heavy metal elements in Surume squid

取得学位:博士（理学）,授与番号:自博甲第874号,授与年月日:2007年3月22日,授与大学:金沢大学,論文主査:山本政

Development of low level plutonium measurement by ICP mass spectrometry and its application to marine environment : Pu isotopes and heavy metal elements in surume squid

取得学位：博士(工学)，学位授与番号：博甲第874号，学位授与年月日：平成19年3月22

Nuclear Localization of COX-2 in relation to the Expression of Stemness Markers in Urinary Bladder Cancer

Inflammation may activate stem cells via prostaglandin E2 (PGE2) production mediated by cyclooxygenase-2 (COX-2) expression. We performed an immunohistochemical analysis of the expression of stemness markers (Oct3/4 and CD44v6) and COX-2 in urinary bladder tissues obtained from cystitis and cancer patients with and without Schistosoma haematobium infections. Immunoreactivity to Oct3/4 was significantly higher in S. haematobium-associated cystitis and cancer tissues than in normal tissues. CD44v6 expression was significantly higher in bladder cancer without S. haematobium than in normal tissues. COX-2 was located in the cytoplasmic membrane, cytoplasm, and nucleus of the cancer cells. Interestingly, the nuclear localization of COX-2, which was reported to function as a transcription factor, was significantly associated with the upregulation of Oct3/4 and CD44v6 in bladder cancer tissues with and without S. haematobium infection, respectively. COX-2 activation may be involved in inflammation-mediated stem cell proliferation/differentiation in urinary bladder carcinogenesis

ケッセイ シンキン マーカー ソクテイ セイド ト ユウヨウセイ

当院臨床検査部では,依頼された検査が診療に役立つように精度管理を徹底し,検査方法の改善や測定の迅速化などを検討している.今回,心筋トロポニンIとT(cTnIとcTnT),ミオグロビン(Mb),CK-MB蛋白(CK-MB mass),CK-MB活性測定(CK-MB),心房ナトリウム利尿ペプチド(ANP),脳性ナトリウム利尿ペプチド(BNP)の検査方法,精度,特性について急性及び慢性心疾患患者の依頼検体265試料を用いて検討した.その結果,cTnIはcTnTと良好な相関関係を示し,測定値はcTnTに比べ約8倍の高値を認めた.急性心筋梗塞(AMI)患者検体のcTnI,Mb,CK-MB massおよびCK-MBは良好な変動を示した.cTnTはcTnIに比べると上昇率が小さく変動も少なかったが,Cut off値と比較するとほぼ同様な変化を認めた.AMI以外の慢性心疾患でもcTnI,CK-MB mass,ANP,BNPは基準値を超える測定値を示した.今回の結果から,心疾患の潜在的な進行や悪化が疑われた場合はcTnIとBNPの測定が他の心筋マーカーと同様に有意義であり,今後も検査部では優れた測定精度と迅速な検査法の検討を続ける必要があることが示唆された.Our goals are to maintain the accuracy of various medical tests, to improve test methods, and to reduce the measurement time. In this study we examined the method, accuracy and time required for measurement of cardiac troponins (cTnT and cTnI), myoglobin (Mb), CK- MB protein mass (CK-MB mass), atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) using 265 samples of patients with acute and chronic heart disease. The coefficient of variation (C.V%) was measured of cTnI (1.9%, 2.2%), cTnT (2.4%, 2.5%), Mb (1.2%, 1.7%), CK-MBmass (1.6%, 2.6%), ANP (5.6%, 7.6%) and BNP (7.1%, 9.4%), and CK-MB (2.1%). cTnI had a good correlation with cTnT (r=0.845, P<0.001 ; n=120) and showed approximately 8-fold high concentrations compared with cTnT. In patients with acute myocardial infarction (AMI), changes in cTnI, Mb and CK-MB mass showed good sensitivity in the early stage, whereas cTnT showed little change. cTnI, CK-MB mass, ANP and BNP were also mostly positive in patients with chronic heart disease, excluding AMI. The results of this study considered that measurement of cardiac markers is useful in patients with heart disease. The sensitivity, accuracy and measurement time for such assays should be further improved, and the utility of the assay items should be evaluated