EDB オ リヨウ シタ ガクシュウ ケイロ タンサク オ シエン スル e シラバス システム ノ コウチク

徳島大学では、教育・研究者情報データベース（EDB)が稼働しており、シラバスの作成やWeb閲覧に利用してきた。現在、教育を学習の基盤（教学インフラ）開発の１つとして、授業科目間の関連性を動的にグラフ表示する。eシラバスシステムの構築を行っている。eシラバスの構築にあたり、各授業科目のシラバスに、キーワード、先行科目、関連科目の３つの項目を新たに設けた。EDBのデータ保存形式であるXMLは汎用性が高く、１つのテキストファイルで構造化データを表現できるため、配布されるCD-ROMにそのまま格納することで、Webを介さずに科目間の関連性をグラフ表示可能である。eシラバスの実現により、ネットワークを介して学習計画や学習経路探索が支援され、シラバスの使い勝手が向上する。In Tokushima University, the syllabus has been published and opened on the web using an Educator and Researcher Information Database (EDB). We are now developing an e-Syllabus system that represents graphically and dynamically the relationships between lectures. This development represents one of the improvements of infrastructure for education and learning. In order to construct the e-Syllabus, three new columns have been added to each lecture of the syllabus: keywords, preceding lectures and relational lectures. XML is used as a storage format for EDB; it allows us to represent the structured data within on a text file that can be stored on a CD-ROM. Thus, without accessing the web, it is possible to represent graphically and discover the relations between the lectures. This representation of the syllabus supports the students in the discovery of learning-paths and the definition of learning plan. Thus, the e-Syllabus system improves the convenience of using the syllabus

Cell jamming, stratification and p63 expression in cultivated human corneal epithelial cell sheets

Corneal limbal epithelial stem cell transplantation using cultivated human corneal epithelial cell sheets has been used successfully to treat limbal stem cell deficiencies. Here we report an investigation into the quality of cultivated human corneal epithelial cell sheets using time-lapse imaging of the cell culture process every 20 minutes over 14 days to ascertain the level of cell jamming, a phenomenon in which cells become smaller, more rounded and less actively expansive. In parallel, we also assessed the expression of p63, an important corneal epithelial stem cell marker. The occurrence of cell jamming was variable and transient, but was invariably associated with a thickening and stratification of the cell sheet. p63 was present in all expanding cell sheets in the first 9 days of culture, but it’s presence did not always correlate with stratification of the cell sheet. Nor did p63 expression necessarily persist in stratified cell sheets. An assessment of cell jamming, therefore, can shed significant light on the quality and regenerative potential of cultivated human corneal epithelial cell sheets

Clinical trial of autologous cultivated limbal epithelial cell sheet transplantation for patients with limbal stem cell deficiency

Objective or Purpose To confirm the efficacy and safety of Good Manufacturing Practice (GMP)-compliant autologous cultivated limbal epithelial cell sheets in government-controlled clinical trials that adhered to Good Clinical Practice stipulations for patients with unilateral limbal stem cell deficiency (LSCD). Design A prospective, multi-center, open-label, uncontrolled, single-arm clinical trial. Subjects, Participants or Controls Ten consecutive eyes of 10 patients with unilateral LSCD were followed for two years after surgery. Preoperative LSCD stage was IIB in four eyes and III in six eyes. Methods A limbal tissue biopsy was obtained from the healthy eye, after which limbal stem cells were dissociated and cultivated on temperature-responsive culture surfaces. All cell sheets were fabricated in a GMP-grade facility under established standard operating procedures. Cell sheets were evaluated using defined shipment criteria before transplantation, and only those that met the criteria were used. The cell sheet was transplanted onto each of the patients' diseased eye after removing the conjunctival scar tissue that covered the corneal surface. The severity of LSCD was determined according to a staging method agreed upon by global consensus, with eyes evaluated as being in stages IA–C representing successful corneal epithelial reconstruction. LSCD diagnosis and staging were determined by the trial's Eligibility Judgment Committee and Effect Assessment Committee using slit-lamp photographs including fluorescein staining. Both committees comprised two or three third-party cornea specialists, who were provided with information anonymously and randomly. Main Outcome Measure Corneal epithelial reconstruction rate was the primary endpoint. Results Corneal epithelial reconstruction was successful in six of 10 eyes (60%) one year postoperatively and was significantly higher than the 15% clinically significant efficacy rate achieved by allogeneic limbal transplantation. The reconstruction rate was 70% of eyes two years postoperatively. Additionally, improvements in visual acuity were noted in 50% and 60% of eyes at one and two years, respectively. No clinically significant transplantation-related adverse events were observed. Conclusion The efficacy and safety of cultivated limbal epithelial cell sheet transplantation were thus confirmed, and the cell sheet, named Nepic, is now approved as a Cellular and Tissue-Based Product in Japan