836 research outputs found

    Proteolytic processing of human cytomegalovirus glycoprotein B (gpUL55) is mediatedby the human endoprotease furin

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    AbstractInhibition of endoproteolytic cleavage of glycoprotein B (gB; gpUL55) of human cytomegalovirus was achieved by treatmentof infected fibroblasts with decanoyl peptidyl chloromethyl ketone (decRVKR-CMK), which inhibits the action of cellular subtilisin-like endoproteases with the amino acid recognition motif R × K/R R. Uncleaved gB precusor molecules of 160 kDa that were accumulated were endoglycosidase H resistant, suggesting that correct cellular transport occurred in the presence of the drug. The inhibitor also prevented endoproteolytic gB processing in CV-1 cells infected with a recombinant vaccinia virus-gB construct (VVgB). Evidence for direct involvement of the ubiquitous subtilisin-like endoprotease furin in gB cleavage was obtained from the observation that coinfection of CV-1 cells with WgB and a recombinant vaccinia-human furin construct reestablished endoproteolytic activity which was normally absent late after infection with WgB alone

    Design Study of a Superconducting Insertion Quadrupole Magnet for the Large Hadron Collider

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    The conceptual design study of a high gradient super conducting insertion quadrupole magnet has been carried out in collaboration between KEK and CERN for the Large Hadron Collider (LHC) to be built at CERN. A model magnet design has been optimized to provide a nominal design field gradient of 240 T/m with a bore aperture of 70 mm and an operational field gradient of 225 T/m at 1.9 K under radiation environment with a beam energy deposit of several watts per meter in the superconducting coils. The design and its optimization process are discussed

    Test Results of a Variant-Design LHC Twin-Aperture Dipole Magnet

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    Since 1989, KEK and CERN carried out jointly an experimental program in the frame of the R&D work for the LHC main dipole. The mechanical structure of this design is based on a separate coil/collar and "horizontally split iron" concept. A total of four single aperture and two twin-aperture 1 m long dipole magnets were built. The last twin-aperture magnet was tested at CERN, reaching a maximum field of 9.55 T at 1.9 K. This paper reports the magnet training performance and quench localization at 1.9 K and 4.5 K. The performance as a function of current ramp rate and measurements of the field quality are also reported
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