Dust release from cold ring particles as a mechanism of spoke formation in Saturn's rings

Spokes in Saturn's rings are radially-extended structures consisting of dust grains. Although spacecraft and space telescope observations have revealed various detailed features of the spokes and their time variation, their formation mechanism is still under debate. Previous models examined charging mechanisms to attempt at explaining dust release from cm-sized ring particles; however, the attempt has been unsuccessful, because the electrostatic force caused by such charging mechanisms is much weaker than the cohesive force acting on dust grains at ordinary conditions in the ring environment. Here we propose a novel model for the formation of the spokes, where the temperature dependence of cohesion plays an essential role. Ring particles with a temperature below 60K adsorb an O2 ring atmosphere, which facilitates release of dust grains from them by a reduction in the cohesive force between the grains and the particles on the morning ansa. Then, intense electrostatic forces sufficient to overcome the cohesive force are generated on the surface of ring particles and the released dust grains form the structure of spokes. Our model explains observational features of the spokes including their longitudinal location, lifetime, radial expansion velocity, and seasonality.Comment: 37 pages, 7 figure

Enhancing the cellular uptake of Py–Im polyamides through next-generation aryl turns

Pyrrole–imidazole (Py–Im) hairpin polyamides are a class of programmable, sequence-specific DNA binding oligomers capable of disrupting protein–DNA interactions and modulating gene expression in living cells. Methods to control the cellular uptake and nuclear localization of these compounds are essential to their application as molecular probes or therapeutic agents. Here, we explore modifications of the hairpin γ-aminobutyric acid turn unit as a means to enhance cellular uptake and biological activity. Remarkably, introduction of a simple aryl group at the turn potentiates the biological effects of a polyamide targeting the sequence 5′-WGWWCW-3′ (W = A/T) by up to two orders of magnitude. Confocal microscopy and quantitative flow cytometry analysis suggest this enhanced potency is due to increased nuclear uptake. Finally, we explore the generality of this approach and find that aryl-turn modifications enhance the uptake of all polyamides tested, while having a variable effect on the upper limit of polyamide nuclear accumulation. Overall this provides a step forward for controlling the intracellular concentration of Py–Im polyamides that will prove valuable for future applications in which biological potency is essential