2 research outputs found
Candida dubliniensis in Japanese Oral Microbiota: A Cross-Sectional Study of Six Geographic Regions in Japan
Introduction: Candida dubliniensis was reclassified from the C. albicans genotype D, and
reports show its frequent detection in HIV-positive individuals and easy acquisition of antifungal
drug resistance. However, the oral carriage rate in healthy people and contribution to candidiasis
in Japan is unclear. Methods: We conducted a cross-sectional survey of the C. dubliniensis carriage
rate, performed genotyping and tested antifungal drug susceptibility and protease productivity.
Specimens from 2432 Japanese subjects in six regions (1902 healthy individuals, 423 with candidiasis
individuals, 107 HIV-positive individuals) were cultured using CHROMagarTMCandida, and the
species was confirmed via 25S rDNA amplification and ITS sequences analyzed for genotyping.
Results: The C. dubliniensis carriage rate in healthy Japanese was low in the central mainland (0–15%)
but high in the most northerly and southerly areas (30–40%). The distribution of these frequencies
did not differ depending on age or disease (HIV-infection, candidiasis). Genotype I, previously
identified in other countries, was most frequent in Japan, but novel genotypes were also observed.
Six antifungal drugs showed higher susceptibility against C. albicans, but protease productivity was
low. Conclusions: Oral C. dubliniensis has low pathogenicity with distribution properties attributed
to geography and not dependent on age or disease status