Myosin 1E localizes to actin polymerization sites in lamellipodia, affecting actin dynamics and adhesion formation

Because the actin network in active lamellipodia is continuously assembling at the edge, moving inward and disassembling, there is a question as to how actin-binding proteins and other components are transported to the leading edge and how nascent adhesions are stabilized. Active transport could play a significant role in these functions but the components involved are unknown. We show here that Myosin 1E (a long tailed Myosin 1 isoform) rapidly moves to the tips of active lamellipodia and to actin-rich early adhesions, unlike Myosin 1G, 1B or 1C (short tailed isoforms). Myosin 1E co-localizes with CARMIL, FHOD1, Arp3 and β3-integrin in those early adhesions. But these structures precede stable paxillin-rich adhesions. Myosin 1E movement depends upon actin-binding domains and the presence of an SH3 oligomerization domain. Overexpression of a Myosin 1E deletion mutant without the extreme C-terminal interacting (SH3) domain (Myosin 1EΔSH3) increases edge fluctuations and decreases stable adhesion lifetimes. In contrast, overexpression of Myosin 1E full tail domain (TH1+TH2+TH3/SH3) decreases edge fluctuation. In Myosin 1E knockdown cells, and more prominently in cells treated with Myosin 1 inhibitor, cell-matrix adhesions are also short-lived and fail to mature. We suggest that, by moving to actin polymerization sites and early adhesion sites in active lamellipodia, Myosin 1E might play important roles in transporting not only important polymerizing proteins but also proteins involved in adhesion stabilization.link_to_OA_fulltex

Chemische Nutzung heimischer Pflanzenoele: Bereitstellung neuartiger Fettstoffe Abschlussbericht

Available from TIB Hannover: DtF QN1(72,29) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Ernaehrung, Landwirtschaft und Forsten, Bonn (Germany)DEGerman

Florida 4-H Senior Horse Record Book

The purpose of the 4-H Horse Program is to provide young people an opportunity to participate in a series of activities designed to improve citizenship, sportsmanship, horsemanship, character, competitive spirit, discipline, and responsibility, while creating an atmosphere for learning and awareness of the life around us. This new 24-page publication of the UF/IFAS 4-H Youth Development Program was written by Wendy DeVito, Saundra TenBroeck, Shane Michael, Megan Brew, and Alyssa Ohmstede. https://edis.ifas.ufl.edu/4h41

Florida 4-H Senior Horse Record Book

The purpose of the 4-H Horse Program is to provide young people an opportunity to participate in a series of activities designed to improve citizenship, sportsmanship, horsemanship, character, competitive spirit, discipline, and responsibility, while creating an atmosphere for learning and awareness of the life around us. This new 24-page publication of the UF/IFAS 4-H Youth Development Program was written by Wendy DeVito, Saundra TenBroeck, Shane Michael, Megan Brew, and Alyssa Ohmstede.https://edis.ifas.ufl.edu/4h418</jats:p