Identification of joint parameters using FRF based decoupling

Structural and mechanical systems are assembled from smaller components using mechanical joints. The mechanical properties of the joints must be modeled in order to perform subsequent design and analysis of the structure or mechanical system. This study provides an analytical method of determining the parameters that describe the behavior of the joints using frequency response function (FRF) data that is measured at joint nodes. The variation in FRFs is derived by utilizing the consistent response conditions at the same joint nodes of the assembly system and the portioned subsystems. The variation reflects the mechanical properties of the joint and is utilized to extract the joint parameters. The validity of the proposed method is illustrated in two numerical applications

Influence of Calcium Sulfate Type on Evolution of Reaction Products and Strength in NaOH- and CaO-Activated Ground Granulated Blast-Furnace Slag

This study investigated the influences of CaSO4 type (i.e., anhydrite vs. gypsum) on strength development and reaction products in the activation of ground granulated blast-furnace slag (GGBFS) when different activators (i.e., CaO vs. NaOH) and sources of GGBFS were used. In the CaO-activation, the addition of calcium sulfates greatly enhanced 28-day strengths, regardless of the choice of CaSO4 or GGBFS source, through increasing the quantities of reaction products and reducing pore volume and size. However, in the NaOH-activation, the use of calcium sulfates showed the complex dependency of strength on the choice of CaSO4 type and GGBFS source, and it barely produced beneficial effects on the quantity of reaction products and reduction of pore volume and size. Thus, the results in this study indicate that the combination of CaO-activation and calcium sulfates is a more effective means of activating GGBFS to gain enhanced strength and significant quality control than the use of gypsum with NaOH-activation

Ethanol Extract of the Flower Chrysanthemum morifolium Augments Pentobarbital-Induced Sleep Behaviors: Involvement of Cl− Channel Activation

Dried Chrysanthemum morifolium flowers have traditionally been used in Korea for the treatment of insomnia. This study was performed to investigate whether the ethanol extract of Chrysanthemum morifolium flowers (EFC) enhances pentobarbital-induced sleep behaviors. EFC prolonged sleep time induced by pentobarbital similar to muscimol, a GABAA receptors agonist. EFC also increased sleep rate and sleep time when administrated with pentobarbital at a subhypnotic dosage. Both EFC and pentobarbital increased chloride (Cl−) influx in primary cultured cerebellar granule cells. EFC increased glutamic acid decarboxylase (GAD) expression levels, but had no effect on the expression of α1-, β2-, and γ2-subunits of the GABAA receptor in the hippocampus of a mouse brain. This is in contrast to treatment with pentobarbital, which showed decreased α1-subunit expression and no change in GAD expression. In conclusion, EFC augments pentobarbital-induced sleep behaviors; these effects may result from Cl− channel activation

Effect of Silica Fume on the Volume Expansion of Metakaolin-Based Geopolymer Considering the Silicon-to-Aluminum Molar Ratio

This paper investigates the effect of silica fume on the mechanical properties of metakaolin-based geopolymers with different silicon-to-aluminum molar (S/A) ratios. Geopolymer has been extensively studied as an alternative to traditional cementitious material because of its low CO2 emissions. Previous studies revealed that the application of silica fume can improve the compressive strength of geopolymer, however, the optimum dosages are different. To examine the reason for the different optimum dosages of silica fume, this study prepares geopolymer specimens of which variables are the S/A ratio and silica fume dosage, and conducts compressive strength and initial setting time tests. To examine whether the strength degradation is caused by the expansion due to the added silica fume in geopolymer, the volume and dynamic modulus are also measured. The results show that a part of silica fume dissolves and changes the S/A ratio of geopolymer, and that a part of silica fume remains in the geopolymer matrix. These combined effects of silica fume result in an irregular compressive strength trend, and, thus, an optimum dosage of silica fume can vary depending on the S/A ratio. Furthermore, the volume expansion of geopolymer with silica fume is observed, however, no sign of damage on the compressive strength is found

Bilateral tension pneumothorax caused by an abrupt increase in airway pressure during cervical spine surgery in the prone position -A case report-

Elevated peak inspiratory airway pressure (PIP) can occur during general anesthesia and is usually easily rectified. In rare circumstances it can lead to potentially fatal conditions such as tension pneumothorax. We report on a 77-year-old male patient admitted for a cervical laminoplasty. The preoperative chest radiograph showed normal findings and there was no medical history of allergy or underlying airway inflammation. Anesthesia induction and maintenance progressed uneventfully. However, 5 minutes after prophylactic antibiotic administration, PIP suddenly increased and blood pressure dropped. The operation was abandoned and the patient was moved to a supine position to perform chest radiography. Cardiac arrest occurred, and cardiopulmonary resuscitation was performed. The radiograph showed bilateral tension pneumothorax. Needle aspiration was immediately performed, and chest tubes were inserted. Ventilation rapidly improved and the vital signs normalized. The patient was discharged without sequelae on postoperative day 36

Transfer Path Analysis of Output Noise Using Multi- dimensional Spectral Analysis Method For Vacuum Cleaner

ABSTRACT Noise reduction of vacuum cleaner is important, according as estimating quality of product. To reduce noise of vacuum cleaner, we need analysis of correct noise source and contribution grasping about Identified noise sources' output noise. Because noise sources' correlation exists in vacuum cleaner that is small and complicated system, and analysis is not easy. In this case, we need to apply Multi-dimensional spectral analysis (MDSA) method that can remove correlation among noise sources and grasp pure contribution degree of noise sources. In this study, we analyze transfer path analysis between output noise and input noise that measured in inside/outside of vacuum cleaner

Development of selective blockers for Ca2+-activated Cl- channel using Xenopus laevis oocytes with an improved drug screening strategy

<p>Abstract</p> <p>Background</p> <p>Ca²⁺-activated Cl^-channels (CaCCs) participate in many important physiological processes. However, the lack of effective and selective blockers has hindered the study of these channels, mostly due to the lack of good assay system. Here, we have developed a reliable drug screening method for better blockers of CaCCs, using the endogeneous CaCCs in <it>Xenopus laevis </it>oocytes and two-electrode voltage-clamp (TEVC) technique.</p> <p>Results</p> <p>Oocytes were prepared with a treatment of Ca²⁺ionophore, which was followed by a treatment of thapsigargin which depletes Ca²⁺stores to eliminate any contribution of Ca²⁺release. TEVC was performed with micropipette containing chelerythrine to prevent PKC dependent run-up or run-down. Under these conditions, Ca²⁺-activated Cl^-currents induced by bath application of Ca²⁺to oocytes showed stable peak amplitude when repetitively activated, allowing us to test several concentrations of a test compound from one oocyte. Inhibitory activities of commercially available blockers and synthesized anthranilic acid derivatives were tested using this method. As a result, newly synthesized <it>N</it>-(4-trifluoromethylphenyl)anthranilic acid with trifluoromethyl group (-CF₃) at <it>para </it>position on the benzene ring showed the lowest IC₅₀.</p> <p>Conclusion</p> <p>Our results provide an optimal drug screening strategy suitable for high throughput screening, and propose <it>N</it>-(4-trifluoromethylphenyl)anthranilic acid as an improved CaCC blocker.</p

Cell-free synthesis of functional phospholipase A1 from Serratia sp.

Additional file 1: Figure S1 Gas chromatography analysis of sesame oil incubated with cell-free synthesized PLA1