Effect of endodontic access simulation on the fracture strength of lithium-disilicate and resin-matrix ceramic CAD-CAM crowns

OBJECTIVES The purpose of this in vitro study was to compare the effect of simulated endodontic access preparation on the failure loads of lithium disilicate crowns and resin-matrix ceramic (RMC) crowns. MATERIALS AND METHODS Eighty maxillary first premolar crowns were manufactured by using four different CAD/CAM blocks (n = 20): lithium disilicate (LD; IPS e.max CAD), resin nanoceramic (RNC; Lava Ultimate), flexible nanaoceramic (FNC; GC Cerasmart), and polymer-infiltrated ceramic (PIC; VITA Enamic). Half of each group was accessed and repaired to simulate endodontic treatment. After cyclic loading, all specimens were loaded to failure. Data were analyzed with two-way ANOVA followed by Tukey-HSD test (α = .05). RESULTS The load to failure results showed significant differences for material types (P < .001), but not for endodontic access simulation (P = .09). The highest and lowest mean failure loads were obtained for LD (1546 N) and PIC (843 N), respectively. CONCLUSION The endodontic access preparation was not found to affect the fracture strength of LD and RMC crowns. The LD showed higher fracture strength than RMC crowns. Even though significant differences were noted for failure loads regarding different crown materials, all could reasonably withstand masticatory forces. CLINICAL SIGNIFICANCE The endodontic access preparation through a restoration is known to be a common challenge in clinical practice. Maintaining a repaired LD or RMC crown is feasible and replacement may not be necessary

Effect of various polymerization protocols on the cytotoxicity of conventional and self-adhesive resin-based luting cements

OBJECTIVES This study evaluated the cytotoxicity of resin-based luting cements on fibroblast cells using different polymerization protocols. MATERIALS AND METHODS Two conventional dual-polymerized (RelyX ARC, VariolinkN) and two self-adhesive resin cements (RelyX Unicem, Multilink Speed) specimens were polymerized using four different polymerization protocols: (a) photo-polymerization with direct light application, (b) photo-polymerization over ceramic and (c) resin nano-ceramic discs and (d) auto-polymerization. The specimens were then assigned to four groups to test cytotoxicity at 0, 1, 2 and 7 preincubation days (n = 5). MTT test was performed using NIH/3T3 fibroblast cells. Data were analysed using three- and one-way ANOVA. Multiple comparisons were made using Bonferroni post hoc test (p < 0.05). RESULTS The highest cytotoxic values were recorded at day 2 for conventional resin cements and at day 0 for self-adhesive resin cements. Self-adhesive resin cements showed the most cytotoxic effect at the second day, while conventional resin cements presented immediate cytotoxicity. Auto-polymerized resin specimens and especially Multilink Speed demonstrated the most cytotoxic effect regardless of the preincubation time. Cytotoxicity of cements tested reached the lowest level at day 7. Interposition of ceramic or nano-ceramic restorative material did not significantly affect the cytotoxicity of tested luting cements (p > 0.05). CONCLUSIONS Cytotoxicity of dual-polymerized resin cements was material-dependent and decreased gradually up to 7 days. Photo-polymerization plays an important role in reducing the cytotoxic effects. CLINICAL RELEVANCE When luting ceramic or resin nano-ceramic restorations of which thickness does not exceed 2 mm, the level of cytotoxicity with the tested materials is not significant. Luting of restorative materials that do not allow for light transmission such as metal-fused porcelain, clinicians should be cautious in the use of dual-polymerized conventional resin cements as only auto-polymerization of resin cements takes place under such materials