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Taha Toros Arşivi, Dosya No: 220-Kaptanzade Ali Rıza Be

<i>An</i>. <i>gambiae</i> Giles abdomen crushing in paper filter Whatman to performed the blind tests according blood meal source.

<p><i>An</i>. <i>gambiae</i> Giles abdomen crushing in paper filter Whatman to performed the blind tests according blood meal source.</p

Patients enrollment and treatments underwent.

<p>Patients enrollment and treatments underwent.</p

MALDI-TOF MS spectra from <i>An</i>. <i>gambiae</i> Giles (<i>Ang</i>) abdomen protein extracts engorged on vertebrate host bloods and then crushed on Whatman filter papers (WFPs).

<p>The MS spectra were generated according to two protocols (P1, P2). Intact <i>Ang</i> match the MS profiles from <i>Anopheles gambiae</i> Giles abdomens crushed on WFPs. The blood-free WFP corresponds to the MS profiles of WFPs where no mosquito blood meal was released. The vertebrate host bloods used for <i>Anopheles gambiae</i> Giles bloody Whatman filter papers (bloody WFPs) were human and sheep. All mosquitoes were collected 12 hours after feeding. a.u. arbitrary units; m/z mass-to-charge ratio.</p

Whatman filter storage method for mosquito blood meal identification.

<p>Comparison of LSVs obtained following a reference TP database query with MS spectra of <i>An</i>. <i>gambiae Giles</i> fed on cow and goat blood. All specimens were collected 12 hours after feeding and stored up to 90 days (D). The mosquito abdominal proteins crushed on WFPs were stored under three different conditions: -20°C, + 4°C and room temperature. The dashed line represents the threshold value for relevant identification (LSVs >1.8). LSV, log score value.</p

Factors influencing patients withdrawal: univariate and multivariable analysis of the 596 treated people with epilepsy.

<p>Factors influencing patients withdrawal: univariate and multivariable analysis of the 596 treated people with epilepsy.</p

Blind tests of the <i>An</i>. <i>gambiae</i> Giles used to set up the protocol optimal for blood meals identification by MALDI TOF from BWFPs.

<p>Blind tests of the <i>An</i>. <i>gambiae</i> Giles used to set up the protocol optimal for blood meals identification by MALDI TOF from BWFPs.</p

Characteristics of the 596 patients.

<p>Characteristics of the 596 patients.</p

Interethnic and intraethnic differences in the frequency and activation markers of blood DCs.

<p>PBMCs from the study participants were stained with monoclonal antibodies for subsequent flow cytometric analysis using a FACSCalibur. The samples were categorised as uninfected Dogon (n = 20), infected Dogon (n = 20), uninfected Fulani (n = 13) and infected Fulani (n = 10). An increase was observed of BDCA2⁺ and BDCA3⁺ cells in the circulation of infected Dogon whereas the opposite was seen for the Fulani (Figure 1A). The expression of activation marker HLA-DR (Figure 1B) and CD86 (Figure 1C) was lower in BDCA2⁺ and BDCA3⁺ cells of the Dogon when undergoing infection while it was increased in the Fulani. It is known that DCs travel to the lymph nodes upon activation. Therefore, it is possible that the lower numbers seen in the infected Fulani is a consequence of activation. The boxplots illustrate the medians and the 25^th and 75^th quartile and the whiskers represent the 10% and 90% percentiles. Data were analyzed by Mann-Whitney rank sum test. *; p≤0.05. **;p<0.01. ***; p<0.001.</p

Comparison of MALDI-TOF MS spectra from <i>An</i>. <i>gambiae</i> Giles abdomen protein extracts engorged on vertebrate host bloods and then crushed on Whatman filters.

<p>The MS spectrum alignment was performed by Flex analysis 3.3 software. Intact <i>Ang</i> match the MS profiles from <i>Anopheles gambiae</i> Giles abdomens crushed on WFPs. The vertebrate host bloods used for mosquito blood meals were rabbit, dog, chicken and rat. All mosquitoes were collected 12 hours after feeding. a.u. arbitrary units; m/z mass-to-charge ratio.</p