The relevance of a digital system based on nanop particles biosensors in assessing the antioxidant potential of some foods

Image analysis is a highly topical and perspective method in biomedical research and practice that underlies the development of portable tests for the autonomous detection of external energy sources outside the laboratory. The purpose of this paper is to implement a new method of detecting antioxidants in food, based on digital image acquisition and processing, using an experimental device with a paper-based biochemical sensor, marked with silver nanoparticles. The research consisted of evaluating the antioxidant potential of some teas (no = 5) and commercial red wines (no = 5), using the LabVIEW device and the LabVIEW graphical programming environment for data processing in linear 3D regression, referencing the RGB spatial vector. The results obtained in the testing of tea and red wine varieties had the following hierarchy regarding their antioxidant concentration (expressed in μg / mL gallic equivalents): Twinings traditional afternoon black tea (10.945); Lipton Green Tea (20.450); Lipton forest fruit tea (20.450); Fares forest fruit tea (29.956); Green Tea Earl Gray (34.708) or Recaş wine (15.698); Pinot Noir Folonari wine (15.698); Chateau Malpere wine (20.450); Cabernet Souvignon wine and Fetească Neagră wine (25.203). In conclusion, we appreciate the great outlook of this fast and cheap method, which can be accessed through software installed on your laptop or smartphone, allowing you to read the result with one click, including by non-experts

Vasile Goldiş

ABSTRACT. The main objective of this study was to compare two acetylcysteine based products with identical quantitative and qualitative composition (acetylcysteine 2g, sucrose and vanillin) and identical pharmaceutical form (oral powder). For the equivalence of the two items we chose the dilution profiles method of determined through HPLC. The data obtained was statistically analyzed and for interpretation, the f2 similarity factor was calculated. We resorted to using this test procedure in vitro because through HPLC, we could not determine the plasmatic concentrations of the 10 horses tested in vivo, in the initial implementation of a standardized protocol. Average percentages of the samples of tested and reference products, dissolved for test showed more than 85% after 5 minutes in each of the 3 different pH dissolution media, indicating similar dilution profiles, indicating there was no need for calculating the f2 similarity factor. Based on the obtained in vitro dissolution profiles, identical quantitative and qualitative composition (2 g acetylcysteine in 6 g powder) and the identical pharmaceutical formulation (oral powder) in vivo testing of the two investigated products is not necessary

Clinical remission of type II diabetes at a female dog after surgical sterilization

In dogs and cats develops more frequently the type II diabetes, non-insulin-dependent, generated by peripheral resistance to insulin and often associated with hypercholesterolemia and obesity. In this study we analyzed the beneficial influence of sterilization on the evolution of canine diabetes. The studied case was a overweight female dog (German Sheperd, 9 years old, 33 Kg), that has presented for 8 months diabetic symptoms, dominated by hyperglycemia (160-186 mg/dL) inconsistently associated with glucosuria and constantly with polydipsia and polyphagia. In time these events have worsened despite the implementation of a rigorous program for the control of diabetes, based on appropriate measures of diet, exercise and socializing. Moreover, the emergence of severe genital complications (pyometra/polycystic ovaries) resulted in a emergency extirpation of the whole genital apparatus. During the post-operative examinations, within 3 weeks a significant amendment of diabetic symptomatology was noticed, following that, within two months the blood glucose decreased to 110 mg/dL and the main blood-biochemical parameters returned to physiological limits. In another few weeks the blood sugar level stabilized around 75 mg/dL and diabetic manifestations resolved completely. In conclusion we believe that the results of this case study are a relevant information for clinicians regarding the use of which require sterilization, or other pathologies related to therapy in clinical remission to normalize glycemia and some forms of canine diabetes