Different gene expression profiles of AD293 and HEK293 cell lines that show contrasting susceptibility to apoptosis induced by overexpression of Bim L

Bim is a pro-apoptotic member of the Bcl-2 protein family. Overexpression of Bim proved to be highly cytotoxic for diverse cells.The AD293 cell line is derived directly from the HEK293 cell line but has been transfected with a gene that can improve cell adherence.We found that there was almost no apoptosis seen in Bim L-transfected AD293 cells, but more than half ofBim L-transfected HEK293 cells underwent apoptosis. Suppression subtractive hybridizationwas used to detect the different gene expression profile between these two cell lines. In 192 sequencedpositive clones, there were 30 clones repeating twice or more. Ten genes were selected for identification by semi-quantitative RT-PCR.Thetranscripts of two adhesion-relatedgenes (actin and parvin)and two apoptosis-related genes (cyclin 2 and protein phosphatase 1G) were up-regulated in AD293 cells. These results suggest that the high expression of cell adhesion-related proteins might be responsible for the different apoptosis status after the transfection of Bim L.Our data provide candidate genes responsible for the different apoptosis sensitivity of these two cell lines. Further investigation on thedifferential expression profile between AD293 and HEK293 might improve our understanding of cell apoptosis mechanism

Eye color predicts disagreeableness in North Europeans: support in favor of Frost (2006)

The current study investigates whether eye color provides a marker of Agreeableness in North Europeans. Extrapolating from Frost’s (2006) research uncovering an unusually diverse range of hair and eye color in northern Europe, we tested the hypothesis that light eyed individuals of North European descent would be less agreeable (a personality marker for competitiveness) when compared to their dark eyed counterparts, whereas there would be no such effect for people of European descent in general. The hypothesis was tested in Australia to provide consistent environmental conditions for both groups of people. Results support the hypothesis. Implications and conclusions are discussed

THE NATURE AND TRANSFORMATION OF CORPORATE HEADQUARTERS: A REVIEW OF RECENT LITERATURE AND A RESEARCH AGENDA

In this paper we suggest that the study of attempts to secure headquarters level change may now be of increasing significance. Evidence of a wave of HQ level change is beginning to accumulate and requires further investigation. The twin purposes of this paper are: (1) in descriptive terms, to gather such evidence as is already available about the nature and extent of HQ level change; to assess the strengths and weaknesses of the extant literature; to suggest where further descriptive work needs to take place; and (2) in explanatory terms, to build some initial and alternative organizational and managerial theories that could inform the study of HQ change; to propose a medium-term research agenda that can be tested in the field