Occurrence of Localized Intense Cation Uptake Sites in the Vascular Rings of Red Beet Storage Tissue 2

Development and decline of cation uptake capacity in discs taken from the vascular and parenchyma rings of storage tissue of red table beet (Beta vulgaris L.) were observed during 12 d of ageing. Uptake capacity for Na+ and Rb+ showed a steady rise reaching maximums by the fourth to fifth days of ageing. Thereafter, there was a steady decline in the uptake rates. Vascular ring tissues were able to develop a greater uptake capacity for both Na+ and Rb+ than the tissues of parenchyma rings. This difference, which was more pronounced for Rb+ than for Na+ uptake, is attributed to a combination of variations in cell density and differences in the acquisition and retention of the cation uptake capacity. Respiration of tissue discs showed no significant rise during ageing, nor were there significant differences in the respiration of vascular and parenchyma tissues. Vascular tissues contained significantly more betacyanin than parenchyma tissues; and they retained their pigment, as well as their acquired cation uptake capacity, for a longer period during the ageing proces

The prediction of macrophyte species occurrence in Swiss ponds

The study attempted to model the abundance of aquatic plant species recorded in a range of ponds in Switzerland. A stratified sample of 80 ponds, distributed all over the country, provided input data for model development. Of the 154 species recorded, 45 were selected for modelling. A total of 14 environmental parameters were preselected as candidate explanatory variables. Two types of statistical tools were used to explore the data and to develop the predictive models: linear regression (LR) and generalized additive models (GAMs). Six LR species models had a reasonable predictive ability (30-50% of variance explained by the selected predictors). There was a gradient in the quality of the 45 GAM models. Ten species models exhibited both a good fit and statistical robustness: Lemnaminor, Phragmitesaustralis, Lysimachiavulgaris, Galiumpalustre, Lysimachianummularia, Irispseudacorus, Lythrumsalicaria, Lycopuseuropaeus, Phalarisarundinacea, Alismaplantago-aquatica, Schoenoplectuslacustris, Carexnigra. Altitude appeared to be a key explanatory variable in most of the species models. In some cases, the degree to which the shore was shaded, connectivity between water bodies, pond area, mineral nitrogen levels, pond age, pond depth, and the extent of agriculture or pasture in the catchment were selected as additional explanatory variables. The species models demonstrated that it is possible to predict species abundance of aquatic macrophytes and that each species responded individually to distinct environmental variable

Eutrophication: are mayflies (Ephemeroptera) good bioindicators for ponds?

Ephemeroptera larvae are recognized worldwide for their sensitivity to oxygen depletion in running waters, and are therefore commonly used as bioindicators in many monitoring programmes. Mayflies inhabiting lentic waters, like lakes and ponds, in contrary have been poorly prospected in biomonitoring. For this purpose, a better understanding of their distribution in lentic habitats and of the relations of species presence with environmental conditions are needed. Within this framework, 104 ponds were sampled in Switzerland. The Ephemeroptera are found to be an insect order particularly well represented in the ponds studied here (93% of the lowland ponds). Nevertheless, in terms of diversity, they are relatively poorly represented (mean species number=1.9). Two species dominated: Cloeon dipterum (Baetidae) and Caenis horaria (Caenidae). The investigations contributed to the updating of the geographical distribution of the species in Switzerland, as many of the observations appear to be from new localities. The trophic state of ponds appears here to be important for Ephemeroptera communities. First, there is a negative relationship between total phosphorus (TP) concentrations and species richness. Second, the presence of Caenis horaria or Cloeon dipterum is dependent on the trophic state. Caenis horaria is most closely associated with low levels of TP concentrations, while Cloeon dipterum appears to be less sensitive, and is most frequently found in hypertrophic conditions. A probable consequence of these relations, is that Baetidae are always present when Caenidae are also present. Contrastingly, Baetidae is observed as the only mayflies family present in several pond

Long-term outcomes of breast cancer patients after endoscopic axillary lymph node dissection: a prospective analysis of 52 patients

Summary: Background. Reports on long-term outcomes after endoscopic axillary lymph node dissection (ALND) of breast cancer patients are still lacking in the medical literature. The objective of this prospective study was to assess the oncological and functional outcomes in breast cancer patients after endoscopic ALND. Methods. Fifty-five breast cancer patients were prospectively enrolled, of whom 52 were available for follow-up with a median of 71.9 months (range 11-96). The following oncological and functional endpoints were evaluated during follow-up at several time points: occurrence of local, axillary and distant metastases, seroma or infection, shoulder mobility (range of motion), numbness, pain, presence of lymphoedema as well as restriction in activities of daily living. Results. In 52 patients endoscopic ALND of level I and II was successfully performed. Two port-site metastases (2/52, 4%) occurred, one of which in a patient with negative axillary lymph nodes. The same patient suffered from the only axillary recurrence (1/52, 2%). Three patients (3/52, 6%) developed lymphoedema. No other functional adverse events (shoulder mobility, pain, numbness, hypertrophic scar) were noticed at the end of the observation period. Conclusion. The present investigation with long-term follow-up after endoscopic ALND - the first one in the literature - reveals minor morbidity, good functional and cosmetic results. In contrary to conventional surgery, the endoscopic procedure is associated with the occurrence of port-site metastases, not seen in the open approach. Axillary recurrences do not appear more frequently when compared with results after conventional ALND. In the meantime the less invasive sentinel lymph node (SLN) biopsy is the established standard technique in evaluating the axillary lymph node statu

Reclassification of the polyphyletic genus Prosthecomicrobium to form two novel genera, Vasilyevaea gen. nov. and Bauldia gen. nov. with four new combinations: Vasilyevaea enhydra comb. nov., Vasilyevaea mishustinii comb. nov., Bauldia consociata comb. nov. and Bauldia litoralis comb. nov.

Species of the genus Prosthecomicrobium are noted for their numerous cellular appendages or prosthecae that extend from the cells. This investigation confirms that the genus is polyphyletic based on an extensive analysis of the 16S rRNA gene sequences of several named species of the genus. The analyses indicate that some Prosthecomicrobium species are more closely related to non-prosthecate genera, including Devosia, Labrenzia, Blastochloris, Methylosinus, Mesorhizobium and Kaistia, than they are to other species of the genus Prosthecomicrobium. For this reason, two of the Prosthecomicrobium clades which are polyphyletic with the type species, Prosthecomicrobium pneumaticum, are renamed as new genera. The currently named species Prosthecomicrobium enhydrum, Prosthecomicrobium mishustinii, Prosthecomicrobium consociatum and Prosthecomicrobium litoralum are reclassified in two new genera, Vasilyevaea gen. nov. and Bauldia gen. nov. with four new combinations: Vasilyevaea enhydra comb. nov. (the type species) and Vasilyevaea mishustinii comb. nov., and Bauldia consociata comb. nov. and Bauldia litoralis comb. nov. (the type species). The type strain of Vasilyevaea enhydra is strain 9bT (=ATCC 23634T =VKM B-1376T). The type strain of the other species in this genus is Vasilyevaea mishustinii strain 17T (=VKM B-2499T =CCM 7569T). The type strain of Bauldia litoralis is strain 524-16T (= NCIB 2233T =ATCC 35022T). The type strain of the other species in this genus is Bauldia consociata strain 11T (=VKM B-2498T =CCM 7594T)

Challenges and opportunities in the use of ponds and pondscapes as Nature-based Solutions

Ponds and “pondscapes” (networks of ponds) are crucial habitats for biodiversity and for delivering multiple benefits to humans, so-called “Nature’s Contribution to People”, such as climate mitigation and adaptation to climate change, creation, and maintenance of habitat for biodiversity, water purification, flood mitigation and cultural benefits (e.g., recreational possibilities). However, ponds are not often considered as Nature-based Solutions to provide all these benefits. In addition, there is insufficient knowledge on how to manage and restore ponds to maximise their role to increase the resilience of ecosystems and society to climate change. To facilitate improved implementation of ponds as Nature-based Solutions for the delivery of a wide range of Nature Contributions to People, it is important to generate and integrate biodiversity, ecosystems, societal, economic and policy knowledge. Hence, there is a need for evidence-based guidance to support the broader use of ponds. Here, we review the role of ponds and pondscapes in delivering Nature’s Contributions to People and provide an overview of the challenges and opportunities for their broader implementation as Nature-based Solutions. Finally, we propose a conceptual framework that can help the implementation of pond Nature-based Solutions, and that outlines future research needs

Temporal dynamics of aquatic communities and implications for pond conservation

Conservation through the protection of particular habitats is predicated on the assumption that the conservation value of those habitats is stable. We test this assumption for ponds by investigating temporal variation in macroinvertebrate and macrophyte communities over a 10-year period in northwest England. We surveyed 51 ponds in northern England in 1995/6 and again in 2006, identifying all macrophytes (167 species) and all macroinvertebrates (221 species, excluding Diptera) to species. The alpha-diversity, beta-diversity and conservation value of these ponds were compared between surveys. We find that invertebrate species richness increased from an average of 29. 5 species to 39. 8 species between surveys. Invertebrate gamma-diversity also increased between the two surveys from 181 species to 201 species. However, this increase in diversity was accompanied by a decrease in beta-diversity. Plant alpha-, beta and gamma-diversity remained approximately constant between the two periods. However, increased proportions of grass species and a complete loss of charophytes suggests that the communities are undergoing succession. Conservation value was not correlated between sampling periods in either plants or invertebrates. This was confirmed by comparing ponds that had been disturbed with those that had no history of disturbance to demonstrate that levels of correlation between surveys were approximately equal in each group of ponds. This study has three important conservation implications: (i) a pond with high diversity or high conservation value may not remain that way and so it is unwise to base pond conservation measures upon protecting currently-speciose habitats; (ii) maximising pond gamma-diversity requires a combination of late and early succession ponds, especially for invertebrates; and (iii) invertebrate and plant communities in ponds may require different management strategies if succession occurs at varying rates in the two groups

New policy directions for global pond conservation

© 2018 Wiley Periodicals, Inc. Despite the existence of well-established international environmental and nature conservation policies (e.g., the Ramsar Convention and Convention on Biological Diversity) ponds are largely missing from national and international legislation and policy frameworks. Ponds are among the most biodiverse and ecologically important freshwater habitats, and their value lies not only in individual ponds, but more importantly, in networks of ponds (pondscapes). Ponds make an important contribution to society through the ecosystem services they provide, with effective conservation of pondscapes essential to ensuring that these services are maintained. Implementation of current pond conservation through individual site designations does not function at the landscape scale, where ponds contribute most to biodiversity. Conservation and management of pondscapes should complement current national and international nature conservation and water policy/legislation, as pondscapes can provide species protection in landscapes where large-scale traditional conservation areas cannot be established (e.g., urban or agricultural landscapes). We propose practical steps for the effective incorporation or enhancement of ponds within five policy areas: through open water sustainable urban drainage systems in urban planning, increased incentives in agrienvironment schemes, curriculum inclusion in education, emphasis on ecological scale in mitigation measures following anthropogenic developments, and the inclusion of pondscapes in conservation policy