A geographic information system for local public health policy

This book deals with the development and use of a geographic information system for local public health policy. Health differences between populations in different geographical areas, large (countries) or small (city-neighbourhoods) have always been a challenge to epidemiologists and policy makers. To epidemiologists, these differences can give insight into the health effects of certain exposures, for policy makers these differences can give rise to welltargeted interventions. Making epidemiological knowledge available to and suitable for policy makers is a major task of the Municipal Health Service. A description of the health situation by means of a community diagnosis can accommodate the requirements of the local politicians. However, it is necessary that a community diagnosis is provided not only for the total municipality, but also for separate districts and neighbourhoods. For this purpose the Municipal Health Service in Rotterdam started the development of a geographic information system. Three questions form a central part in this study: 1. Is it possible to set up a geographic information system, in order to obtain insight into the health situation in neighbourhoods? 2. Can such a geographic information system be used for epidemiological research? 3. Can such a geographic information system be used for developing and supporting local health policy? In the first part of the study attention is focused on recent developments in health policy, the concept of a community diagnosis and the potentials of geographic information systems for local health policy. In the second part of the study the theory is worked out for the situation in Rotterdam

Characterization of novel components of the baculovirus per os infectivity factor complex

Baculovirus occlusion-derived virus (ODV) infects insect midgut cells under alkaline conditions, a process mediated by highly conserved per os infectivity factors (PIFs), P74 (PIF0), PIF1, PIF2, PIF3, PIF4, and PIF5 (ODV-E56). Previously, a multimolecular complex composed of PIF1, PIF2, PIF3, and P74 was identified which was proposed to play an essential role during ODV entry. Recently, more proteins have been identified that play important roles in ODV oral infectivity, including PIF4, PIF5, and SF58, which might work in concert with previously known PIFs to facilitate ODV infection. In order to understand the ODV entry mechanism, the identification of all components of the PIF complex is crucial. Hence, the aim of this study was to identify additional components of the PIF complex. Coimmunoprecipitation (CoIP) combined with proteomic analysis was used to identify the components of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) PIF complex. PIF4 and P95 (AC83) were identified as components of the PIF complex while PIF5 was not, and this was confirmed with blue native PAGE and a second CoIP. Deletion of the pif4 gene impaired complex formation, but deletion of pif5 did not. Differentially denaturing SDS-PAGE further revealed that PIF4 forms a stable complex with PIF1, PIF2, and PIF3. P95 and P74 are more loosely associated with this complex. Three other proteins, AC5, AC68, and AC108 (homologue of SF58), were also found by the proteomic analysis to be associated with the PIF complex. Finally the functional significance of the PIF protein interactions is discussed

Recent natural selection causes adaptive evolution of an avian polygenic trait

We used extensive data from a long-term study of great tits (Parus major) in the United Kingdom and Netherlands to better understand how genetic signatures of selection translate into variation in fitness and phenotypes. We found that genomic regions under differential selection contained candidate genes for bill morphology and used genetic architecture analyses to confirm that these genes, especially the collagen gene COL4A5, explained variation in bill length. COL4A5 variation was associated with reproductive success, which, combined with spatiotemporal patterns of bill length, suggested ongoing selection for longer bills in the United Kingdom. Last, bill length and COL4A5 variation were associated with usage of feeders, suggesting that longer bills may have evolved in the United Kingdom as a response to supplementary feeding

Parity Violation in Elastic Electron-Nucleon Scattering: Strangeness Content in the Nucleon

Parity violation in elastic electron-nucleon scattering is studied with the basic goal of improving the understanding of electroweak hadronic structure with special emphasis on the strangeness content in the nucleon. Models for the parity-violating (PV) asymmetry are provided and compared with the world data measured at very different kinematics. The effects introduced in the PV asymmetry due to alternative descriptions of the hadronic structure are analyzed in detail. In particular, a wide selection of prescriptions for dealing with the electromagnetic and neutral current weak interaction nucleon form factors, including the most recent ones used in the literature, is considered.Comment: 73 pages, 20 figures. To be published in Physics Report

Proteomic markers with prognostic impact on outcome of chronic lymphocytic leukemia patients under chemo-immunotherapy: results from the HOVON 109 study

Despite recent identification of several prognostic markers, there is still a need for new prognostic parameters able to predict clinical outcome in chronic lymphocytic leukemia (CLL) patients. Here, we aimed to validate the prognostic ability of known (proteomic) markers measured pretreatment and to search for new proteomic markers that might be related to treatment response in CLL. To this end, baseline serum samples of 51 CLL patients treated with chemo-immunotherapy were analyzed for 360 proteomic markers, using Olink technology. Median event-free survival (EFS) was 23 months (range: 1.25–60.9). Patients with high levels of sCD23 (>11.27, p = 0.026), sCD27 (>11.03, p = 0.04), SPINT1 (>1.6, p = 0.001), and LY9 (>8.22, p = 0.0003) had a shorter EFS than those with marker levels below the median. The effect of sCD23 on EFS differed between immunoglobulin heavy chain variable gene-mutated and unmutated patients, with the shortest EFS for unmutated CLL patients with sCD23 levels above the median. Taken together, our results validate the prognostic impact of sCD23 and highlight SPINT1 and LY9 as possible promising markers for treatment response in CLL patients

Q

The Qweak experiment, which took data at Jefferson Lab in the period 2010 - 2012, will precisely determine the weak charge of the proton by measuring the parity-violating asymmetry in elastic e-p scattering at 1.1 GeV using a longitudinally polarized electron beam and a liquid hydrogen target at a low momentum transfer of Q2 = 0.025 (GeV/c)2. The weak charge of the proton is predicted by the Standard Model and any significant deviation would indicate physics beyond the Standard Model. The technical challenges and experimental apparatus for measuring the weak charge of the proton will be discussed, as well as the method of extracting the weak charge of the proton. The results from a small subset of the data, that has been published, will also be presented. Furthermore an update will be given of the current status of the data analysis