New positive, tetrazolium-linked, staining method for use with electrophoresis of phosphoglycerate kinase

Eine Methode zur positiven Anfärbung der Phosphoglycerat-Kinase (EC 2.7.2.3) nach Stärkegelektrophorese wird beschrieben. Das in der Vorwärtsreaktion des Enzyms gebildete Adenosin-Triphosphat wird mit einem Indikatorsystem (Hexokinase, Glukose-6-phosphat-Dehydrogenase, Tetrazoliumsalz) erfasst.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42513/1/18_2005_Article_BF01928301.pd

The effects of zinc on the morphology of sickle red blood cell ghosts as observed by scanning electron microscopy

The objective of these studies is to define the role of zinc in RBC ghost morphology specifically in comparison to the echinocytogenic effects of calcium. Erythrocyte ghosts were prepared from patients with sickle cell anemia, treated with zinc and/or calcium and the resulting cell morphology studied by scanning electron microscopy. Our results showed that zinc consistently and significantly antagonized the echinocytogenic effect of calcium. A simple log linear statistical model was used to evaluate the data and supported this conclusion. We discuss the potential significance of these findings to the effect of zinc treatment on patients with sickle cell anemia.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47537/1/433_2005_Article_BF01851386.pd

Studies of enzyme polymorphisms in the Kamuela population of Drosophila mercatorum . II. Evaluation of glycolytic intermediates

A simple and effective cryogenic procedure for the extraction of glycolytic intermediates from whole Drosophila has been developed. This procedure gives consistent results when a measure (µM/liter/OD 260 ) is adopted which corrects for differences in extraction efficiency. Using this measure and a homozygous strain of D. mercatorum , there are no significant differences among extracts for the levels of any of the 15 glycolytic intermediate or energy molecules considered. The profile of means is consistent across experimental designs and instrument types. Coefficients of variation are well below 50% for most variables. The methodology presented has the statistical power to detect a mean change of 10 to 50% using an experimental design which requires as few as 32 observations. The estimated energy charge for resting Drosophila from these studies is the expected value of 0.86.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44142/1/10528_2004_Article_BF00498934.pd

IN VIVO RED CELL GLYCOLYTIC CONTROL AND DPG-ATP LEVELS *

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73406/1/j.1749-6632.1974.tb21907.x.pd