2 research outputs found
Kinetics of plasma cellâfree DNA and creatine kinase in a canine model of tissue injury
Background:
Cellâfree DNA (cfDNA) comprises short, doubleâstranded circulating DNA sequences released from damaged cells. In people, cfDNA concentrations correlate well with disease severity and tissue damage. No reports are available regarding cfDNA kinetics in dogs.
Objectives/Hypothesis:
Cellâfree DNA will have a short biological halfâlife and would be able to stratify mild, moderate, and severe tissue injury. Our study aims were to determine the kinetics and biological halfâlife of cfDNA and to contrast them with those of creatine kinase (CK).
Animals:
Three groups of 10 dogs undergoing open ovariohysterectomy, surgery for cranial cruciate ligament rupture (CCLR), or hemilaminectomy.
Methods:
Plasma for cfDNA and CK analysis was collected at admission, at induction of anesthesia, postsurgery (time 0) and at 6, 12, 24, 36, 48, 60, and 72 hours after surgery.
Results:
The biological halfâlife of plasma cfDNA and CK were 5.64 hours (95% confidence interval [CI 95], 4.36â7.98 hours) and 28.7 hours (CI95, 25.3â33.3 hours), respectively. In the hemilaminectomy group, cfDNA concentrations differed significantly from admission at 6â12 hours after surgery. Creatine kinase activity differed among the surgical groups and reached a peak 6 hours after surgery. In the ovariohysterectomy and CCLR groups, plasma CK activity 72 hours after surgery did not differ from admission activity of the ovariohysterectomy group. In contrast, in the hemilaminectomy group, plasma CK activity after 72 hours did not return to the ovariohysterectomy group admission activity.
Conclusions and Clinical Importance:
Plasma CK activity has a longer biological halfâlife than previously thought. In contrast to plasma CK activity, cfDNA has a short halfâlife and could be a useful marker for peracute severe tissue injury