Beam Sensitivity of Globoid Crystals within Seed Protein Bodies and Commercially Prepared Phytates During X-Ray Microanalysis

Magnesium, potassium, calcium and phosphorus are stored in seed tissues in spherical particles called globoid crystals. The main component of globoid crystals is phytin, a salt of myo-inositol hexaphosphoric acid. Based on chemical similarity, commercially available phytates may be suitable standards for quantitative energy dispersive X-ray (EDX) microanalysis of globoid crystals. The stability of globoid crystals and commercial phytates was different when analyzed under identical conditions. Phytates in globoid crystals from Cucurbita maxima cotyledons, were relatively stable in the electron beam during EDX microanalysis at room temperature, but there was a loss of potassium. No loss of potassium occurred during analysis at low temperature, even with repeated analyses on the same spot. Commercial phytates showed considerable beam damage in the form of raised mounds at the sites of analyses. The extent of the damage was much reduced with analysis at low temperature. Although there was some variation in the peak-to-background ratios of potassium and sodium with various analytical conditions, there was no differential loss of potassium or sodium as occurred with EDX analysis of globoid crystals. Reasons for differences between in situ and isolated phytates are unclear. Provided analyses are carried out at low temperature, commercial phytates have potential as standards but further research is required to determine how to control moisture content during sample preparation and analysis

Surviving the Next Generation Console Transition

During the next three years the video game industry will be challenged by the transition to a new generation of game consoles. The increased development costs associated with an order of magnitude leap in console performance is the game developer\u27s main concern. As retail prices of console games will not increase, the implication is a shakeout of smaller game companies. However, during the transition period other gaming platforms will gain market share from the consoles, offering new opportunities for companies positioned to exploit them. The changing landscape will require game developers such as Radical Games to revise their strategies, as Radical\u27s traditional competitive advantages will be eroded by the transition. Radical should diversify from its primary focus on consoles and explore the emerging niche opportunities, using the transition as an opportunity to develop its own Intellectual Property in a low-cost, low-risk manner

The role of point-of-care ultrasound in the assessment of schistosomiasis-induced liver fibrosis: a systematic scoping review

Background Abdominal ultrasound imaging is an important method for hepatic schistosomiasis diagnosis and staging. Several ultrasound staging systems have been proposed, each attempting to standardise schistosomal periportal fibrosis (PPF) diagnosis. This review aims to establish the role of ultrasound in the diagnosis and staging of schistosomal PPF, and to map the evolution of ultrasound staging systems over time, focusing on internal validation and external reproducibility. Methods A systematic search was undertaken on 21st December 2022 considering the following databases: PubMed/MEDLINE (1946-present), Embase (1974-present), Global Health (1973-present), Global Index Medicus (1901-present), and Web of Science Core Collection–Science Citation Index Expanded (1900-present) and the Cochrane Central Register of Controlled Trials (1996-present). Case reports, systematic reviews and meta-analyses, and studies exclusively using transient or shear-wave elastography were excluded. Variables extracted included study design, study population, schistosomal PPF characteristics, and diagnostic methods. The PRISMA-ScR (2018) guidelines were followed to inform the structure of the scoping analysis. Results The initial search yielded 573 unique articles, of which 168 were removed after screening titles and abstracts, 43 were not retrieved due to full texts not being available online or through inter-library loans, and 170 were excluded during full text review. There were 192 remaining studies eligible for extraction. Of the extracted studies, 61.8% (76/123) of studies that reported study year were conducted after the year 2000. Over half of all extracted studies (59.4%; 114/192) were conducted in Brazil (26.0%; 50/192), China (18.8%; 36/192) or Egypt (14.6%; 28/192). For the species of schistosome considered, 77.6% (149/192) of studies considered S. mansoni and 21.4% (41/192) of studies considered S. japonicum. The ultrasound staging systems used took on three forms: measurement-based, feature-based and image pattern-based. The Niamey protocol, a measurement and image pattern-based system, was the most used among the staging systems (32.8%; 63/192), despite being the most recently proposed in 1996. The second most used was the Cairo protocol (20.8%; 40/192). Of the studies using the Niamey protocol, 77.8% (49/63) only used the image patterns element. Where ultrasound technology was specified, studies after 2000 were more likely to use convex transducers (43.4%; 33/76) than studies conducted before 2000 (32.7%; 16/49). Reporting on ultrasound-based hepatic diagnoses and their association with clinical severity was poor. Just over half of studies (56.2%; 108/192) reported the personnel acquiring the ultrasound images. A small number (9.4%; 18/192) of studies detailed their methods of image quality assurance, and 13.0% (25/192) referenced, discussed or quantified the inter- or intra-observer variation of the staging system that was used. Conclusions The exclusive use of the image patterns in many studies despite lack of specific acquisition guidance, the increasing number of studies over time that conduct ultrasound staging of schistosomal PPF, and the advances in ultrasound technology used since 2000 all indicate a need to consider an update to the Niamey protocol. The protocol update should simplify and prioritise what is to be assessed, advise on who is to conduct the ultrasound examination, and procedures for improved standardisation and external reproducibility

Successfully translating conservation research into practice and policy: concluding thoughts

In the Anthropocene, when our environment is changing rapidly and the windows of opportunity for action to prevent further biodiversity loss are narrow, conservation researchers are increasingly encouraged to think and operate beyond the traditional approaches of producing peer-reviewed papers and presenting results to other members of the research community. Indeed, the perception that researchers belong in their ivory tower, from which they deliver evidence for others to interpret, disseminate and use in decision-making, is thankfully now widely recognised as outdated. The rise of fake news, a deliberate lack of consideration for scientific evidence, and changes to the ways of assessing the value of researchers’ work probably all play a role in supporting this shift in perception. Moreover, for many researchers, the prospect of their work ‘making a difference’ and having an impact on wider society is at least as great a motivation for doing research as generating new knowledge, however interesting that may be

Exploring new ways of thinking about and developing staff practice: the role of modes of thinking

Purpose: Nobel Prize winner Daniel Kahneman’s work on modes of “thinking” provides a comprehensive text which is little explored in respect of work with people who have an intellectual or developmental disability. This paper aims to explore the potential of this work to change staff development and practice. Design/Methodology: Key themes from Thinking Fast, and Slow (Kahneman, 2011) are described and applied to current staff practice. Findings: Modes of thinking are relevant and important to understanding and improving manager and staff practice. Originality: To our knowledge this is the first attempt to describe and understand staff thinking and practice using Kahneman’s ideas