Uticaj dodavanja zeolita na kvalitet mesa pastrmke

U radu je ispitivan uticaj zeolita tipa "Minazel" kao aditiva hrane za pastrmke primenjenog u koncentraciji od 1% i 2% na sledeće proizvodne parametre: osnovni hemijski sastav mesa, mikotoksikološku ispravnost, senzorne osobine, randman i koncentraciju pojedinih makro i mikroelemenata. Primenjene koncentracije zeolita, kao aditiva hrane nisu uticale na hemijski sastav pastrmskog mesa, koncentraciju minerala (Ca, Cu, Zn, Pb i Mn) i njegove senzorne osobine. Izvesni stepen pozitivnog uticaja ispoljen je u pogledu randmana mesa i prosečne telesne mase pastrmki posle ezenteracije

T-Cell Infiltration and Adaptive Treg Resistance in Response to Androgen Deprivation With or Without Vaccination in Localized Prostate Cancer

Purpose: Previous studies suggest that androgen deprivation therapy (ADT) promotes antitumor immunity in prostate cancer. Whether a vaccine-based approach can augment this effect remains unknown. Experimental Design: Therefore, we conducted a neoadjuvant, randomized study to quantify the immunologic effects of a granulocyte-macrophage colony-stimulating factor (GM-CSF)-secreting allogeneic cellular vaccine in combination with low-dose cyclophosphamide (Cy/GVAX) followed by degarelix versus degarelix alone in patients with high-risk localized prostate adenocarcinoma who were planned for radical prostatectomy. Results: Both Cy/GVAX plus degarelix and degarelix alone led to significant increases in intratumoral CD8+ T cell infiltration and PD-L1 expression as compared to a cohort of untreated, matched controls. However, the CD8+ T cell infiltrate was accompanied by a proportional increase in regulatory T cells (Treg), suggesting that adaptive Treg resistance may dampen the immunogenicity of ADT. Although Cy/GVAX followed by degarelix was associated with a modest improvement in time-to-PSA progression and time-to-next treatment as well as an increase in PD-L1, there was no difference in the CD8 T-cell infiltrate as compared to degarelix alone. Gene expression profiling demonstrated that CHIT1, a macrophage marker, was differentially upregulated with Cy/GVAX plus degarelix compared to degarelix alone. Conclusions: Our results highlight that ADT with or without Cy/GVAX induces a complex immune response within the prostate tumor microenvironment. These data have important implications for combining ADT with immunotherapy. In particular, our finding that ADT increases both CD8+ T cells and Tregs, supports the development of regimens combining ADT with Treg-depleting agents in the treatment of prostate cancer

The Distributed Boosting Algorithm

In this paper, we propose a general framework for distributed boosting intended for efficient integrating specialized classifiers learned over very large and distributed homogeneous databases that cannot be merged at a single location. Our distributed boosting algorithm can also be used as a parallel classification technique, where a massive database that cannot fit into main computer memory is partitioned into disjoint subsets for a more efficient analysis. In the proposed method, at each boosting round the classifiers are first learned from disjoint datasets and then exchanged amongst the sites. Finally the classifiers are combined into a weighted voting ensemble on each disjoint data set. The ensemble that is applied to an unseen test set represents an ensemble of ensembles built on all distributed sites. In experiments performed on four large data sets the proposed distributed boosting method achieved classification accuracy comparable or even slightly better than the standard boosting algorithm while requiring less memory and less computational time. In addition, the communication overhead of the distributed boosting algorithm is very small making it a viable alternative to the standard boosting for large-scale databases