Performance of the new automated Abbott RealTime MTB assay for rapid detection of Mycobacterium tuberculosis complex in respiratory specimens

The automated high-throughput Abbott RealTime MTB real-time PCR assay has been recently launched for Mycobacterium tuberculosis complex (MTBC) clinical diagnosis. This study would like to evaluate its performance. We first compared its diagnostic performance with the Roche Cobas TaqMan MTB assay on 214 clinical respiratory specimens. Prospective analysis of a total 520 specimens was then performed to further evaluate the Abbott assay. The Abbott assay showed a lower limit of detection at 22.5 AFB/ml, which was more sensitive than the Cobas assay (167.5 AFB/ml). The two assays demonstrated a significant difference in diagnostic performance (McNemar’s test; P = 0.0034), in which the Abbott assay presented significantly higher area under curve (AUC) than the Cobas assay (1.000 vs 0.880; P = 0.0002). The Abbott assay demonstrated extremely low PCR inhibition on clinical respiratory specimens. The automated Abbott assay required only very short manual handling time (0.5 h), which could help to improve the laboratory management. In the prospective analysis, the overall estimates for sensitivity and specificity of the Abbott assay were both 100 % among smear-positive specimens, whereas the smear-negative specimens were 96.7 and 96.1 %, respectively. No cross-reactivity with non-tuberculosis mycobacterial species was observed. The superiority in sensitivity of the Abbott assay for detecting MTBC in smear-negative specimens could further minimize the risk in MTBC false-negative detection. The new Abbott RealTime MTB assay has good diagnostic performance which can be a useful diagnostic tool for rapid MTBC detection in clinical laboratories. © 2015, Springer-Verlag Berlin Heidelberg.postprin

Open-air oxidative mizoroki-heck reaction of arylsulfonyl hydrazides with alkenes

2015-2016 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Phosphine ligand for indole skeleton as well as preparation method and application of phosphine ligand

Inventor name used in this publication: 邝福儿Inventor name used in this publication: 苏秋铭Inventor name used in this publication: 周永健Inventor name used in this publication: 原安莹Title in Traditional Chinese: 一種吲哚骨架的膦配體及其制備方法和應用China202212 bcchVersion of Recor

Palladium-catalyzed cross-coupling of (hetero)aryl or alkenyl sulfonates with aryl titanium as the multi-functional reagent

202308 bckwAccepted ManuscriptRGCOthersNational Natural Science Foundation of China; Shenzhen Strategic New Industry Development Research Fund of Shenzhen Science, Technology and Innovation Commission (SZSTI)Publishe

Palladium-catalyzed direct arylation of polyfluoroarenes for accessing tetra-ortho-substituted biaryls : Buchwald-type ligand having complementary −PPh₂ moiety exhibits better efficiency

202308 bckwAccepted ManuscriptRGCOthersCUHK Direct Grant; The Hong Kong Polytechnic University Start-up Fund; Guangdong Province Zhu Jiang Talents PlanPublishe

Palladium-catalyzed Buchwald-Hartwig amination and Suzuki-Miyaura cross-coupling reaction of aryl mesylates

202308 bckwVersion of RecordRGCPublishe

Synthesis of flavone derivatives through versatile palladium-catalyzed cross-coupling reactions of tosyloxy- and mesyloxyflavones

202308 bckwAccepted ManuscriptOthersHong Kong Polytechnic University Start-up FundPublishe

Reduction of self-quenching effect in organic electrophosphorescence emitting devices via the use of sterically hindered spacers in phosphorescence molecules

The reduction of self-quenching effect by introduction of sterically hindered spacers in phosphorescence molecules was analyzed for a electrophosphorescence emitting device. The iridium complex of tris-ortho-cycloetalated compound exhibits a strong green phosphorescence emission with a photoluminescence (PL) quantum yield of 0.71 in solutions and a short lifetime in solids. Self-quenching is reduced significantly due to sterically hindered pinene spacers which provides minimum bimolecular interaction.link_to_subscribed_fulltex