6 research outputs found

    Genetic differences between Tunisian camel and sheep strains of the cestode Echinococcus granulosus revealed by SSCP

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    Ovine and dromedary Echinococcus granulosus isolates from Tunisia were identified as G1 and G6 strains based on polymorphism of the mitochondrial cytochrome C oxydase CO1. Single strand conformation polymorphism (SSCP) was used in order to examine the genetic variation within and between Tunisian G1 and G6 strains and to estimate the extent of selfing. The dromedary isolates are genetically distinct from sheep isolates (high value of genetic variation between populations: Fst = 0.46). No significant deficiency in heterozygotes was found in sheep isolates, whereas heterozygote deficiency (suggesting selfing) was found in a limited number of camel isolates.Les Echinococcus granulosus de Tunisie ont Ă©tĂ© identifiĂ©s comme appartenant aux lignĂ©es G1 et G6 en se fondant sur le polymorphisme de la cytochrome oxydase CO1. Le polymorphisme de conformation simple brin (SSCP) a Ă©tĂ© utilisĂ© afin d’évaluer la variabilitĂ© gĂ©nĂ©tique intra et inter-isolats des lignĂ©es G1 et G6, et pour estimer le taux d’autofĂ©condation. Les isolats issus de dromadaires sont gĂ©nĂ©tiquement distincts des isolats ovins (valeur de Fst : 0,46). Il n’y a pas de dĂ©ficit significatif en hĂ©tĂ©rozygotes chez les isolats ovins. Un dĂ©ficit en hĂ©tĂ©rozygotes est prĂ©sent dans les isolats du dromadaire, ce qui suggĂšre l’autofĂ©condation comme l’un des modes de reproduction
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