Inhibition of Plasma Membrane Calcium Pump Influences Intracellular Calcium Signaling Pathways in Breast Cancer

The plasma membrane calcium pump (PMCA) is an important transporter that maintains intracellular calcium concentration ([Ca2+](i)). It allows the calcium (Ca2+) from inside the cell to go out of the cell through the plasma membrane. For this, it cooperates with the proteins in the cell. The aim of this study is to demonstrate the effect of PMCA on intracellular calcium signaling in breast cancer cells. In this study, PMCA was inhibited by orthovanadate (OV), and changes in Calmodulin (CaM), Calcineurin (CaN) and cMyc proteins were demonstrated. Intracellular calcium accumulation was measured when PMCA was inhibited in MDA-MB-231 cells. At the same time, it was observed that the cell movement decreased with time. Over time, CaN and CaM were slightly suppressed, and cMyc protein was not expressed. As a result, when PMCA protein is targeted correctly in breast cancer cells, it has an indirect effect on cancer-promoting proteins

The effect of bee bread (Perga) with chemotherapy on MDA-MB-231 cells

Bee bread (BB) is a bee product like propolis and honey. It is the main food for larvae and bees producing royal jelly in the hive. It also known as Perga. As with other bee products, it is increasingly popular due to its antioxidant properties. The aim of this study was to examine the effects of BB on MDA-MB-231 breast cancer cells and the effects on these cells when administered together with Doxorubicin (DOX) and Cisplatin (CDDP), used in cancer treatment. The proliferation of the cells was determined by applying 5 mg/mL BB together with different concentrations of DOX and CDDP. In addition to these studies, the effect of DOX+BB and CDDP+BB combinations on the migration of MDA-MB-231 cells was determined by the wound healing method. The expression levels of Bid and Bcl-2 were determined by RtqPCR. According to these studies, as expected, BB did not show a significant toxic effect on MDA-MB-231 cells at different concentrations. BB significantly suppressed the effect of DOX and CDDP on the proliferation of MDA-MB-231 cells. BB with DOX and CDDP suppressed the proapoptotic Bid gene while overexpressing the anti-apoptotic Bcl-2 gene, separately. Interestingly, BB blocked the migration of MDA-MB-231 cells by 50% even after 72 h. As a result, BB significantly reduced the toxicity of DOX and CDDP on MDA-MB-231 cells. The most interesting result of the study is that BB prevented the migration of cancer cells

Role of the norepinephrine transporter polymorphisms in atomoxetine treatment: From response to side effects in children with ADHD

Objective

The analyze of azospermia factor and cystic fibrozis gene mutations in male infertile individuals with congenital unilateral or bilateral vas deferens agenesis. 8th Balkan Meeting On Human Genetics, Dubrovnik Croatia

Collaborative tagging systems have emerged in recent years to become popular tools for organising information on the Web. While collaborative tagging offers many advantages, they also suffer from several limitations, with a major one being the existence of ambiguous tags. To understand what an ambiguous tag is intended to mean, we need to know the contexts in which it is used. Instead of using common large scale clustering techniques on folksonomies, we believe tags can be better contextualised by the social contexts in which they are used. We propose a method to reveal semantics of ambiguous tags by studying the collective user behaviour in a tagging system. In this paper we describe our proposal and some results of our preliminary experiments. We also discuss the significance of the work and how it can be evaluated

Erythropoietin improves brain development in short-term hypoxia in rat embryo cultures

Background: Hypoxic ischemic encephalopathy continues to be a significant cause of death and disability worldwide. Erythropoietin (EPO) has the potential to lessen neurologic sequelae due to hypoxia ischemia. Methods: The in vitro effects of EPO on total embryonic development and brain VEGF receptor (VEGFR) expressions were investigated in 50 rat embryos at 9.5 days of gestation that were cultured in whole rat serum (WRS). According to the study protocol, the embryos were divided into two groups. The first group is comprised hypoxia, 100 and 50 U/ml EPO after hypoxia groups. Group 2 comprised control (WRS) and WRS EPO. After 48-h culture, the embryos from each group were harvested to be analyzed according to a morphological scoring system and also genetically to measure brain VEGFR expression. Results: The mean morphological scores for the embryos grown in control, WRS + EPO, hypoxia, and in the presence of 100 and 50 U/ml EPO in hypoxic medium were 55.30 +/- 7.22, 52.10 +/- 5.27, 23.0 +/- 4.60, 36.20 +/- 5.07, and 19.70 +/- 5.07, respectively. Expressions of VEGFR-1, -2, -3 were significantly elevated in the 100 Wail EPO and WRS + EPO groups compared to the hypoxia group (p < 0.05). Conclusions: These results support the conclusion that (1) VEGFR-1, -2, -3 may increase with EPO treatment in hypoxic conditions, (2) VEGF and EPO may be part of a selfregulated physiological protection mechanism to prevent neuronal injury including in utero neural tube defects. (C) 2014 The Japanese Society of Child Neurology. Published by Elsevier B.V. All rights reserved