Macular sensitivity and fixation patterns in normal eyes and eyes with uveitis with and without macular edema

PURPOSE: This study aims to investigate the relationship between macular sensitivity and thickness in eyes with uveitic macular edema (UME). DESIGN: This study is a prospective observational case series. METHODS: The setting for this study was clinical practice. The study included 59 (28 with UME, 31 without UME) eyes of 26 patients with uveitis and 19 eyes of 10 normal subjects. The procedure followed was fundus-related perimetry and retinal thickness map with an automated fundus perimetry/tomography system. Main outcome measures included quantification of macular sensitivity, fixation pattern, and relationship between macular sensitivity and thickness. RESULTS: Fixation stability revealed that 56 eyes (93.44%) had stable fixation (\u3e75% within the central 2° of point of fixation); three eyes (6.56%) were relatively unstable (75% located within 4°); and no eye had unstable fixation (50% of fixation point within 0.5 mm of foveal center); seven eyes (11.86%) had peri-central fixation location (25% \u3c 50% within 0.5 mm); and seven eyes (11.86%) had eccentric (280 μm. CONCLUSIONS: Perimetry quantification of macular sensitivity and retinal thickness, in association with other factors, may offer novel information regarding the impact of UME on retinal function

Farmers’ Perception of Climate Change and Coping Strategies across Gender in Two Agro-Ecological Zones of Nigeria

This study examined farmers’ perception of climate change and coping strategies across gender in two agro-ecological zones of Nigeria. Two agro-ecological zones namely north-central and north-west were purposively selected and one State Agricultural Development Project each namely Niger and Kaduna were selected repectively. Structured questionnaire was used to elicit information from 140 respondents. Data were analysed using both descriptive and inferential statistics. Personal experience was the most prominent means through which females (35.7%) and males (38.6%) became aware of climate change. Majority of the females (85.7%) and males (74.3%) in the north-central zone , started to notice the effect of climate change only about 1-5years ago. About 89% of males and 82% of females opined that yearly rainfull begins late. About 97% of the female and 99% of the males were of the opinion that there had been gradual reduction of vegetation cover. General perception by both females (mean=3.2) and males (mean=3.2) was that climate change reduces the yield of crops, increase the cost of crop production and reduces the productivity of livestock and also time spent by the females to look for water for domestic use had increased. Chi-square analysis indicated positive and significant relationship between perception of climate change and farm size (X2 = 4.1; p<0.05) in north- central and farming experience (X2 = 7.3; p<0.05) in north west zone. The results of t-test analysis showed significant difference between how the females and males perceived the effect of climate change with regards to rainfall ending early (t(138)=2.5; p<0.05) and time spent to look for water for cooking food has increased(t(138)=2.2;p<0.05). A multi-media enlightenment campagn of the effects and posssible coping strategies of climate change should be adopted by all tiers of government and NGOs to reach the farmers using available extension structure on ground. Also, farming communities can run local disaster risk committees to encourage local adaptation measures as survival tactics for the purpose of ensuring food security.Key Words: Gender, Farmers Perception, Coping strategies, Climate Change, Agro-ecological Zon

Toll-like receptor 2, 3, and 4 expression and function in human airway smooth muscle

Background: Host defense against microbial pathogens is elicited through the innate immune system by means of Toll-like receptors (TLRs). Airway smooth muscle cells (ASMCs) display proinflammatory and immunomodulatory functions. ASMCs might participate in airway inflammatory responses associated with innate immune activation. Objectives: We determined the effects of cytokines, TLR ligands, and corticosteroids on TLR expression and function in human ASMCs. Methods: Real-time PCR and flow cytometry were used to assess TLR mRNA and protein expression, respectively. ASMCs were stimulated with TLR ligands, and chemokine release was measured by means of ELISA. Results: ASMCs expressed TLR1 to TLR10 mRNA, and TLR2 and TLR3 protein expression was demonstrated. TNF-α and double-stranded RNA (dsRNA; TLR3 ligand) were potent inducers of TLR2 and TLR3 mRNA expression, and both stimuli had additive or synergistic effects with IFN-γ on TLR2 and TLR4, but not TLR3, mRNA expression. Peptidoglycan (TLR2 ligand) and LPS (TLR4 ligand) weakly enhanced TLR2 mRNA expression. Peptidoglycan, dsRNA, and LPS induced IL-8 and eotaxin release, with dsRNA being most potent. dsRNA also modulated cytokine-induced chemokine release in a differential manner. Dexamethasone inhibited cytokine- and ligand-induced TLR2, TLR3, and TLR4 expression and chemokine release. However, dexamethasone potentiated TLR2 expression induced by combined IFN-γ and TNF-α stimulation. Conclusion: Expression of TLR2, TLR3, and TLR4 is regulated by cytokines and TLR ligands, and their activation mediates chemokine release in ASMCs. Clinical implications: Proinflammatory responses mediated by activation of pathogen-recognition receptors in ASMCs might contribute to infectious exacerbations of airway inflammatory conditions, such as asthma and chronic obstructive pulmonary disease. © 2006 American Academy of Allergy, Asthma and Immunology

Effects of Climate Change on Fluted Pumpkin Production and Adaptaton Measures Used Among Farmers in Rivers State

The importance of fluted pumpkin crop in rural household economy cannot be over emphasized. Farmers in the South South Nigeria depend greatly on fluted pumpkin for its many uses. The production however is beset with a myriad of constraints of which climate change is one of the most outstanding. The study assessed the effects of climate change and adaptation measures used by fluted pumpkin farmers in Ikwerre Local Government Area(LGA) of Rivers State, Southsouth Nigeria. Multi-stage sampling technique was used to select respondents for the study. Out of the twelve communities that make up Ikwerre LGA, six communities were randomly chosen from which one hundred and eighty fluted pumpkin farmers were proportionately selected. Interview schedule and focus group discussion were used to elicit in depth information from respondents. Data were analyzed using percentage, frequency count and mean. Tables and charts were used in presentation of data. The result of the study showed that 51 percent of the respondents were female, majority were married (77 percent) and were full time farmers (79 percent). Relatively large proportion had no formal education (42 percent) and indicated both consumption and sale (79 percent) as the major aim of production. The respondents perceived that unpredictable climate condition, changes in rainfall pattern, changes in rainfall distribution, reduced yield of fluted pumpkin and reduction of family income were the major effects of climate change on fluted pumpkin production. A combination of fluted pumpkin production with other income generating activities was shown to be the most widely used adaptation strategy by respondents. Based on the results of the study it was recommended that improved extension services that can provide the needed farming inputs (fluted pumpkin seedlings, fertilizers e.t.c) as well as useful and relevant information on climate change and adaptation strategies should be made available to the people