Medindo O Acesso à Justiça Cível No Brasil

In this article, we map out studies and methodologies used to build subjective indicators of access to justice, focusing on experiences with and management of conflict. We propose a blended measure of access to justice, based on critical analysis of key studies, and work with a broad understanding of what access to justice means and what a lack of access implies. This definition includes the right to a just resolution of conflicts by any appropriate means (not necessarily via state justice) and also takes into account individuals' awareness of their rights. This work relies on survey data collected between 2010 and 2014 and covers the procedural and substantive dimensions of access to justice. We have documented state-of-the-art measurement and composition of indicators of access to justice, noting key limitations and ways to overcome them, and emphasizing construct validity and filters used in the definition of access to justice. We conclude from the proposed blended measure that, depending on where emphasis is placed-on conflict or on seeking institutional justice-the phenomenon being measured will be different, as people react in different ways to different types of potentially conflicting situations.22231834

Novel and conserved microRNAs in soybean floral whorls

AbstractMicroRNAs (miRNAs) correspond to a class of endogenous small non-coding RNAs (19–24nt) that regulates the gene expression, through mRNA target cleavage or translation inhibition. In plants, miRNAs have been shown to play pivotal roles in a wide variety of metabolic and biological processes like plant growth, development, and response to biotic and abiotic stress. Soybean is one of the most important crops worldwide, due to the production of oil and its high protein content. The reproductive phase is considered the most important for soybean yield, which is mainly intended to produce the grains. The identification of miRNAs is not yet saturated in soybean, and there are no studies linking them to the different floral organs. In this study, three different mature soybean floral whorls were used in the construction of sRNA libraries. The sequencing of petal, carpel and stamen libraries generated a total of 10,165,661 sequences. Subsequent analyses identified 200 miRNAs sequences, among which, 41 were novel miRNAs, 80 were conserved soybean miRNAs, 31 were new antisense conserved soybean miRNAs and 46 were soybean miRNAs isoforms. We also found a new miRNA conserved in other plant species, and finally one miRNA-sibling of a soybean conserved miRNA. Conserved and novel miRNAs were evaluated by RT-qPCR. We observed a differential expression across the three whorls for six miRNAs. Computational predicted targets for miRNAs analyzed by RT-qPCR were identified and present functions related to reproductive process in plants. In summary, the increased accumulation of specific and novel miRNAs in different whorls indicates that miRNAs are an important part of the regulatory network in soybean flower

Soybean (Glycine max) as a versatile biocatalyst for organic synthesis

A series of aliphatic and aromatic aldehydes and ketones were reduced using plant cell preparations of Glycine max seeds (soybean). The biotransformation of five aromatic aldehydes in water, at room temperature afforded the corresponding alcohols in excellent yields varying from 89 to 100%. Two prochiral aromatic ketones yielded the alcohol in very low conversion, 1% and to 4%; however with good enantiomeric excess (ee) of 99 and 79%, respectively. Additionally, three prochiral and one cyclic aliphatic ketones produced the corresponding alcohols in moderate yields varying from 10 to 58% and ee varying from 73 to 99%. Hydrolysis of two aromatic esters yielded the expected carboxylic acids in 49 and 66%. Most of the obtained alcohols have commercial value as cosmetic fragrances. Although, the enzymes present in soybean (reductase/lipase) has not been defined, the reaction is an important route for the preparation of pure alcohols and carboxylic acid, with low cost and environmental impact.Keywords: Glycine max, biocatalysis, bioreduction, aldehydes and ketones, ester hydrolysi

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic resonance spectroscopy. Structural analysis indicates that this fucan has a central core consisting mainly of 3-ß-D-glucuronic acid 1-> or 4-ß-D-glucuronic acid 1 ->, substituted at C-2 with alpha-L-fucose or ß-D-xylose. Sulfate groups were only detected at C-3 of 4-alpha-L-fucose 1-> units. The anticoagulant activity of the PF1 (only 2.5-fold lesser than low molecular weight heparin) estimated by activated partial thromboplastin time was completely abolished upon desulfation by solvolysis in dimethyl sulfoxide, indicating that 3-O-sulfation at C-3 of 4-alpha-L-fucose 1-> units is responsible for the anticoagulant activity of the polymer.Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de GlicobiologiaUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros NaturaisUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BioquímicaUNIFESP, EPM, Depto. de BioquímicaSciEL

Hypothalamic Endoplasmic Reticulum Stress Of Overtrained Mice After Recovery

knowing the relationship between endoplasmic reticulum (ER) stress and inflammation and based on the fact that downhill running-based overtraining (OT) model increases hypothalamus levels of some pro-inflammatory cytokines, we verified the effects of three OT protocols on the levels of BiP, pIRE-1 (Ser734), pPERK (Thr981), pelF2alpha (Ser52), ATF-6 and GRP-94 proteins in the mouse hypothalamus after two weeks of recovery. Methods: the mice were randomized into control (CT), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up) and overtrained by running without inclination (OTR) groups. After 2-week total recovery period (i.e., week 10), hypothalamus was removed and used for immunoblotting. Results: The OTR/down group exhibited high levels of BiP and ATF6. The other OT protocols showed higher levels of pPERK (Th981) and pelf-2alpha (Ser52) when compared with the CT group. Conclusion: The current results suggest that after a 2-week total recovery period, the overtrained groups increased partially their ER stress protein levels, but without hypothalamic inflammation, which characterizes a physiological condition related to an adaptation mechanism.2

REVISÃO DE LITERATURA: REANIMAÇÃO CARDIOPULMONAR

RESUMO: Parada cardiorrespiratória (PCR) é a cessação súbita, inesperada e catastrófica da circulação sistêmica. A reanimação cardiopulmonar pode ser considerada o conjunto de manobras realizadas logo após uma PCR com o objetivo de manter artificialmente o fluxo arterial ao cérebro e a outros órgãos vitais, até que ocorra o retorno da circulação espontânea .Este trabalho tem como objetivo realizar revisão de literatura sobre a evolução da ressuscitação cardiopulmonar através dos tempos e discutir as mais novas diretrizes em relação às manobras de reanimação.Existem relatos sobre a ressuscitação cardiopulmonar desde abordagens bíblicas, métodos científicos rudimentares e processos manuais até o desenvolvimento de técnicas como respiração boca a boca e mais recentemente as manobras de suporte básico de vida, as quais tem colaborado para salvar vidas. É importante salientar que além de disseminar estas manobras para os profissionais da saúde, a população leiga deve ser orientada. Uma ressuscitação cardiopulmonar de boa qualidade e precoce influencia a evolução do quadro da vítima de parada cardiorrespiratória, sendo de extrema relevância os avanços obtidos através dos tempos em relação a essa técnica. Portanto, para se alcançar sucesso em relação a reanimação é necessário treinamento, o qual deve ser realizado tanto em ambiente hospitalar para profissionais de saúde como também estimulado para leigos.PALAVRAS CHAVE: Parada Cardiorrespiratória. Reanimação. Treinamento. Ambiente hospitalar

Detection of biogenic amines in mead of social bee

Mead is an alcoholic beverage, resulting from the fermentation of honey and water, although it is an old drink, some fermentation products are still unknown. Biogenic amines have driven research on food and beverages due to their toxicity and capacity to cause damage to the human health. This study aimed to evaluate the presence of biogenic amines in mead from honey of two species of social bees, using different fining agents (bentonite, banana peel flour, and passion fruit peel flour) and storage in oak barrel for 120 days. We observed the presence of three biogenic amines for each type of mead. Putrescine was reported only in samples of mead of Apis mellifera. Tryptamine and histamine were not detected, while tyramine was observed in the mead samples of honey from Melipona quadrifasciata anthidioides. Samples clarified with fruit peels increased the number of amines. In the analysis of the histamine, used as standard, obtained limits of detection and quantification of 2.47 mg/L and of 7.48 mg/L, respectively. In this study, the amines observed remained below the limit of detection, and did not pose a risk to consumers. The finings used demonstrated a profile similar to the control mead, and its use was satisfactory.We thank the National Council of Technological and Scientific Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq) under Grant (305885/2017 to C.A.L.C.). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001. We also thank the Fundação de Amparo a Pesquisa do Estado da Bahia - FAPESB, under Grant 8797/2015.info:eu-repo/semantics/publishedVersio

Synthesis and tomographic characterization of the displaced Fock state of light

Displaced Fock states of the electromagnetic field have been synthesized by overlapping the pulsed optical single-photon Fock state |1> with coherent states on a high-reflection beamsplitter and completely characterized by means of quantum homodyne tomography. The reconstruction reveals highly non-classical properties of displaced Fock states, such as negativity of the Wigner function and photon number oscillations. This is the first time complete tomographic reconstruction has been performed on a highly non-classical optical state