12 research outputs found
Identification and characterization of an isolate of apple green crinkle associated virus involved in a severe disease of quince (Cydonia oblonga, Mill.)
A quince tree showing severe symptoms of a
previously undescribed viral disease occurring in northern
Apulia (Italy) was analysed using high-throughput
sequencing of small RNA libraries, leading to the identification
of a new strain of apple green crinkle associated
virus (isolate AGCaV-CYD) showing peculiar traits. RTPCR
with specific primers detected AGCaV-CYD in consistent
association with symptoms in the surveyed orchards.
Molecular characterization of the reconstructed
genome, together with phylogenetic analysis, showed it to
be closely related to an AGCaV strain causing green
crinkle disease in apple (AGCaV-AUR) and divergent from
the type strain of apple stem pitting virus (ASPV-PA66)