Development of a customised design flood estimation tool to estimate floods in gauged and ungauged catchments

Published ArticleThe estimation of design flood events, i.e., floods characterised by a specific magnitude-frequency relationship, at a particular site in a specific region is necessary for the planning, design and operation of hydraulic structures. Both the occurrence and frequency of flood events, along with the uncertainty involved in the estimation thereof, contribute to the practising engineers’ dilemma to make a single, justifiable decision based on the results obtained from the plethora of ‘outdated’ design flood estimation methods available in South Africa. The objectives of this study were: (i) to review the methods currently used for design flood estimation in South Africa for single-site analysis, (ii) to develop a customised, user-friendly Design Flood Estimation Tool (DFET) containing the latest design rainfall information and recognised estimation methods used in South African flood hydrology, and (iii) to demonstrate the use and functionality of the developed DFET by comparing and assessing the performance of the various design flood estimation methods in gauged catchments with areas ranging from 100 km² to 10 000 km² in the C5 secondary drainage region, South Africa. The results showed that the developed DFET will provide designers with an easy-to-use software tool for the rapid estimation and evaluation of alternative design flood estimation methods currently available in South Africa for applications at a site-specific scale in both gauged/ungauged and small/large catchments. In applying the developed DFET to gauged catchments, the simplified ‘small catchment’ (A ≤ 15 km²) deterministic flood estimation methods provided acceptable results when compared to the probabilistic analyses applicable to all of the catchment sizes and return periods, except for the 2-year return period. Less acceptable results were demonstrated by the ‘medium catchment’ (15 km² 5 000 km²) empirical flood estimation methods. It can be concluded that there is no single design flood estimation method that is superior to all other methods used to address the wide variety of flood magnitude-frequency problems that are encountered in practice. Practising engineers’ still have to apply their own experience and knowledge to these particular problems until the gap between flood research and practice in South Africa is narrowed by improving existing (outdated) design flood estimation methods and/or evaluating methods used internationally and developing new methods for application in South Africa

A new pathway of glucocorticoid action for asthma treatment through the regulation of PTEN expression

<p>Abstract</p> <p>Background</p> <p>"Phosphatase and tensin homolog deleted on chromosome 10" (PTEN) is mostly considered to be a cancer-related gene, and has been suggested to be a new pathway of pathogenesis of asthma. The purpose of this study was to investigate the effects of the glucocorticoid, dexamethasone, on PTEN regulation.</p> <p>Methods</p> <p>OVA-challenged mice were used as an asthma model to investigate the effect of dexamethasone on PTEN regulation. Immunohistochemistry was used to detect expression levels of PTEN protein in lung tissues. The human A549 cell line was used to explore the possible mechanism of action of dexamethasone on human PTEN regulation <it>in vitro</it>. A luciferase reporter construct under the control of PTEN promoter was used to confirm transcriptional regulation in response to dexamethasone.</p> <p>Results</p> <p>PTEN protein was found to be expressed at low levels in lung tissues in asthmatic mice; but the expression was restored after treatment with dexamethasone. In A549 cells, human PTEN was up-regulated by dexamethasone treatment. The promoter-reporter construct confirmed that dexamethasone could regulate human PTEN transcription. Treatment with the histone deacetylase inhibitor, TSA, could increase PTEN expression in A549 cells, while inhibition of histone acetylase (HAT) by anacardic acid attenuated dexamethasone-induced PTEN expression.</p> <p>Conclusions</p> <p>Based on the data a new mechanism is proposed where glucocorticoids treat asthma partly through up-regulation of PTEN expression. The <it>in vitro </it>studies also suggest that the PTEN pathway may be involved in human asthma.</p

HSPF modelling of the Msunduzi River Catchment

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